Labshake search
Citations for abcam :
1 - 50 of 1751 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Phosphate kit: Phosphate Assay Kit (Colorimetric) (Abcam, product code: ab65622). Sodium kit ...
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bioRxiv - Microbiology 2022Quote: ... Potassium kit: Potassium Assay Kit (Fluorometric) (Abcam, product code: ab252904). Osmolarity was measured in each SM sample using an osmometer (Osmomat 030 by Gonotec).
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bioRxiv - Immunology 2020Quote: ... Genotyping PCR products were digested with AvaI restriction enzyme (Abcam, Cambridge, Massachusetts) for 1 hour at 37°C and electrophoresed on a 3% agarose gel in 1X Tris-acetate-EDTA (TAE ...
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bioRxiv - Cancer Biology 2022Quote: ... GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749, abcam) from OAM6#93 (PTA-5704 ...
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bioRxiv - Cancer Biology 2022Quote: ... with anti-GPR64 (purified using Mouse TCS Antibody Purification Kit (ab128749, abcam) from OAM6#93 (PTA-5704 ...
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bioRxiv - Cell Biology 2021Quote: ... Advanced oxidative protein products AOPP Assay Kit was obtained from Abcam (ab242295). AOPP is a simple ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-JMJD3 antibodies (Abgent) were affinity purified using EpiMAX Affinity Purification kit (Abcam) and epitope retrieval was performed with 2N HCl ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were first cleaned using the Abcam Antibody Purification Kit (Protein A) (Abcam, ab102784) following manufacturer’s instructions (performing overnight incubation at 4 °C in the spin cartridge on a rotor at 8 rpm) ...
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bioRxiv - Genomics 2022Quote: ... The ligated product was bisulfite converted using Abcam Fast Bisulfite conversion kit (Abcam, ab117127). The bisulfite converted product was amplified using NEBNext Q5U Master Mix (NEB ...
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bioRxiv - Genetics 2023Quote: ... The total triglycerides were quantified with a triglyceride assay kit (product # ab65336) from Abcam by following the standard protocol offered by the company ...
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bioRxiv - Immunology 2022Quote: ... anti-A20 (product #: ab13597; clone: 59A426) (Abcam) or anti-beta-actin (Product # ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GAPDH (Abcam product code ab8245, 1:2000). Results were analyzed using the Compass Software ...
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bioRxiv - Plant Biology 2021Quote: Quantitative measurement of reactive oxygen species was carried out using DCFDA cellular ROS detection assay kit (Abcam, product number AB113851), and the assay was carried out following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Antibody purification and concentration: The anti-Smarca5 (Abcam, ab72499), and rabbit IgG (Merk-Millipore ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.
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bioRxiv - Cell Biology 2023Quote: ... To be included antibodies had to be available in a protein free format (except for GPS2, which we purified using an Abcam Protein G purification kit). All antibodies selected were known to be specific ...
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bioRxiv - Genomics 2023Quote: ... Cells were regularly tested for mycoplasma contamination using the Mycoplasma PCR Detection Kit (abcam ab289834). E14 mouse embryonic stem cells were a gift from W ...
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bioRxiv - Biochemistry 2019Quote: ... and donkey anti-mouse AF568 (Abcam, product number ab175700). Sections incubated in secondary antibody solution for 2 hours at room temperature with gentle shaking and protected from light ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti BrdU (Abcam, product no: ab6326, (1:100)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti DCX (Abcam, product no: ab77450, (1:100)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti mouse Sox2 (Abcam, product no: ab69893, (1:200)) ...
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bioRxiv - Cancer Biology 2021Quote: ... GMPPN (hydrolysis product of GMPPNP, JenaBioscience, (≥ 95 %)) and GMP (Abcam, (>98 %)) ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-protein gene product 9.5 (1:500, Abcam ab10404). No labelling was observed in control sections where primary antibody was excluded ...
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bioRxiv - Cell Biology 2020Quote: ... CHMP1A (rabbit polyclonal, product ab178686; used at 1/500) was from Abcam; PRC1 (mouse mAb IgG2b ...
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bioRxiv - Neuroscience 2023Quote: ... We used as the primary antibody anti-GFP from abcam (Product # ab13970). We used as the secondary an anti-chicken antibody conjugated to Alexa488 from ThermoFisher (Product # A-11039).
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant human cyclophilin A (product ab86219) was purchased from Abcam (Cambridge, United Kingdom). Cyclosporine A purchased from Merck Life Science (Dorset ...
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bioRxiv - Genetics 2020Quote: ... coli OP50 supplemented with 100 nM (25S)-Δ7-dafachronic acid (Abcam, product ab144236) immediately prior to seeding ...
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bioRxiv - Biochemistry 2023Quote: ... Full length recombinant human osteopontin protein was purchased from Abcam (product number ab87460). Heavy-isotope labelled osteopontin tryptic peptides YPDAVATWLNPDPSQK ...
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bioRxiv - Biophysics 2019Quote: ... Immunohistochemistry for advanced glycation end products (AGE) was carried out with AGE antibody (Abcam, UK). Bound primary antibody was detected using Vectastain ABC kit and NovaRed detection kit (Vector Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 1 µg of α-HA-antibody (ChIP Grade, product # ab9110, Abcam, Cambridge, MA) in 3 ml ice-cold ChIP lysis buffer and incubated for 4 h at 4 ᵒC with rocking ...
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bioRxiv - Biochemistry 2022Quote: ... and the quality of the purification was assessed via SDS-PAGE followed by InstantBlue Coomassie protein stain (abcam: ab119211) staining and immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... immuoprecipitations were conducted using Dynabead magnetic beads (Thermo-Fisher product 10002D) conjugated with anti-FLAG (Abcam ab1162) anti-rabbit monoclonal antibody at 1:250 dilution ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... a polyclonal antibody (1/320 dilution) that binds to all forms of α1-antitrypsin (Abcam product 9373), an anti-mouse IgG (1.5nM ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysis contained GFP tag strain was subjected to affinity purification by using Anti–GFP antibody (ab290, Abcam, Cambridgeshire, UK). The process of antibody purification was using GenScript Protein A MagBeads according to the manufacturer’s instructions (Catalog No ...
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bioRxiv - Plant Biology 2023Quote: ... The purification level of each recombinant enzyme was estimated by analyzing an aliquot (20 µl) of each purification fraction on SDS-PAGE (8% acrylamide) stained with InstantblueTM (Abcam).
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bioRxiv - Synthetic Biology 2021Quote: ... Recombinant gene product was either detected with Goat anti-GFP conjugated to Alkaline Phosphatase (ab6661 Abcam, Cambridge, Massachusetts) at 1:5000 dilution or 1:5000 Rabbit Polyclonal anti-SARS-CoV2 RBD (Cat# 40592-T62 Sino Biological ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared products were collected and incubated in fresh Protein A agarose beads and anti-huntingtin EPR5526 (Abcam) for 2 hours rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The stability of the recombinant protein during the purification was monitored by SDS-PAGE and Western blot analysis (WB) using a rabbit anti-CSB primary antibody (ABCAM: ab96089) and a goat anti-rabbit HRP secondary antibody (ABCAM ...
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bioRxiv - Immunology 2021Quote: ... Purified products were separated by SDS-PAGE and after blocking for Western Blot either murine PGRN antibody (abcam#ab169325) followed by anti-mouse/POX 1:3000 (Biorad#170-6516) ...
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bioRxiv - Cancer Biology 2023Quote: ... fragmented and run-on reaction products were immuno-purified two times using anti-Br-UTP antibody (ab-6326, Abcam) bound to 30 μl of magnetic beads (Protein G DynabeadsThermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Mycoplasma contamination was ruled out by PCR (Abcam 289834).
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Microbiology 2024Quote: ... A commercial kit (Immunoprecipitation Kit, Abcam), which provided buffers for protein extraction and solubilization as well as Protein A/G sepharose beads and washing buffers ...
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bioRxiv - Cell Biology 2020Quote: ... with fibronectin-depleted (depleted via column purification using gelatin sepharose) FBS at 10% on glass coverslips coated with 10ug/mL murine fibronectin (native mouse fibronectin, Abcam, Cambridge MA, USA) or laminin (laminin mouse protein ...
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bioRxiv - Cell Biology 2021Quote: ... ∼ 5×1010 EVs collected from the two steps of purification were diluted into 500 μl of PBS and 2 μg of rabbit monoclonal anti-CD9 (Abcam, Cambridge, U.K., ab92726) or rabbit IgG (Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... The amounts of active Rap-1 were detected by immunoblot analysis of the pull-down product using anti-Rap1 goat polyclonal antibody (Abcam, ab212011).
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bioRxiv - Bioengineering 2023Quote: ... 1 mg/ml collagen gels were incubated with 10mM MGO and the clinically relevant MGO-derived advanced glycation end-products (AGE) hydroimidazolone MG-H1 was measured using a competitive ELISA assay (Abcam, UK) according to the manufacturer’s instructions and previously published approaches23.