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Citations for abcam :
551 - 600 of 10000+ citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493). Blots were washed three times with TBST and once with TBS ...
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Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila developmentbioRxiv - Biochemistry 2022Quote: ... and incubated with a 1% nonfat milk/PBST solution containing the primary antibodies anti-AOX (rabbit polyclonal, 1:20,000) (10) and anti-ATP5A (mouse monoclonal, Abcam, UK, 1:10,000) for 3 h at room temperature under constant shaking ...
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bioRxiv - Neuroscience 2021Quote: ... 0.2% BSA in PBS), and resuspended in antibody buffer (0.1% saponin, 1% BSA in PBS) containing rabbit anti-TBR2 (Abcam Cat# ab23345, RRID:AB_778267) antibody and incubated for 1 hr at 4°C with rocking ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed once with 200 μL PBS containing 1% BSA and incubated with PE conjugated Goat F(ab’)2 Anti-Human IgG-(Fab’)2 secondary antibody (abcam, Cambridge, MA) at a final concentration of 2.5 μg/ml in 100 μL PBS containing 1% BSA per well ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing chicken anti-GFP polyclonal antibody (1:1000; Abcam, Cambridge, MA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was washed in 1x PBS containing 0.02% Tween-20 three times and incubated with horseradish peroxidase-labeled goat anti-rabbit antibody (Abcam catalog numbe ab205718) at a 1:5000 dilution for 1 hour (Lisha et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary mouse HRP-conjugated antibody (Abcam Inc., Cambridge, MA). All incubations and wash steps were performed at room temperature unless otherwise stated ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked with PBS containing 0.1% saponin and 1% BSA for 30 min and incubated with primary mouse anti-LAMP1 antibody (Abcam, ab25630, 1:3000) or rabbit anti-CTSD antibody (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was first blocked in PBS 1X 0,02% Tween-20 containing 5% Milk and incubated overnight with the dsDNA-specific antibody (ab27156 Abcam, dilution 1/1000). To quantify G4 formation ...
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bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were blocked in PBTx (1% Triton X-100 in PBS) containing 3% bovine serum albumin and incubated over three nights with chicken anti-GFP antibody (Abcam Cat#ab13790). After several washes in PBTx ...
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bioRxiv - Bioengineering 2023Quote: ... we replaced the blocking buffer with a fresh 250uL of the same blocking buffer containing a 1:1000 dilution of our primary antibody against ACE2 (polyclonal Rabbit-anti-hACE2, Abcam, Cat# Ab15348). Following a 1 hour incubation at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing chicken anti-GFP polyclonal antibody (1:1000; Abcam, Cambridge, MA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam, Cambridge, MA) for 90 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... washed three times with PBS and then 200 µl PBS containing anti-human antibodies conjugated to alkaline phosphatase (Abcam, 1:2000 dilution) was added for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881, 1:50; IgG, Abcam ab46540, 1:100). Primary antibody incubation was done by incubating the beads at 4 °C with gentle nutation overnight ...
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bioRxiv - Biochemistry 2023Quote: ... containing 10 µM DCFDA (Abcam), a fluorescent indicator of ROS ...
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bioRxiv - Cancer Biology 2024Quote: ... Periodic Acid Schiff/Alcian Blue (PAS/AB) staining was performed per the manufacturer’s instructions (Abcam, ab245876). For standard IHC on human tissue sections ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were plated at 200,000 cells/coverslip with the addition of 10 μM Rho-Kinase Inhibitor Y-27632 (10 μM; Abcam, Cambridge, UK; Ab120129). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Plant Biology 2019Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... 1:1000 dilution in blocking buffer) plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919 ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Plant Biology 2019Quote: ... Protein detection was performed using a monoclonal rabbit anti-VEGF primary antibody (1:1000 dilution, ab52917, Abcam plc, Cambridge, UK) and a goat anti-rabbit HRP secondary antibody (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... 2019) using antibodies against cAMP response element-binding protein (CREB; clone LB9, produced in mouse, #ab178322, AbCam, Cambridge, UK; RRID:AB_2827810), phospho-CREB (Ser133 ...