Mouse Glycerol kinase GK ELISA Kit citations for abcam

Quote: Tissue levels of MMP12 in Hi-Myc and healthy FVB/NJ prostate homogenates were measured by ELISA according to manufacturer’s protocol (ab246540, abcam). Prostate homogenates were prepared as previously described ...

Microglia Mediate Early Corticostriatal Synapse Loss and Cognitive Dysfunction in Huntington’s Disease Through Complement-Dependent Mechanisms

D.K. Wilton, et al., bioRxiv - Neuroscience 2021

Quote: 16 μg of protein extracted from frozen human tissue samples was employed in a hemoglobin sandwich ELISA (Abcam Ab157707). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.

Conserved epigenetic programming and enhanced heme metabolism drive memory B cell reactivation

Madeline J. Price, et al., bioRxiv - Immunology 2021

Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...

Sexual dimorphism of the synovial transcriptome underpins greater PTOA disease severity in male mice following joint injury

Rachel F. Bergman, et al., bioRxiv - Physiology 2022

Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...

On Casein Kinase-2 (CK2) deregulation in NSCLC: an enzyme subunit-centered approach

George V. Pérez, et al., bioRxiv - Cancer Biology 2023

Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

P López-Jiménez, et al., bioRxiv - Cell Biology 2022

Quote: ... SYCP1 was detected with a mouse monoclonal antibody against mouse SYCP1 (Abcam, ab15090) at a 1:50 dilution ...

Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosis

Wesley Freppel, et al., bioRxiv - Microbiology 2023

Quote: ... Mouse anti-VDAC1 (ab14734) and mouse anti-GRP75 (ab2799) were obtained from Abcam. Rabbit anti-IP3R1 (NBP2-22458 ...

Complement Regulator Factor H is a Cofactor for Thrombin in both Pro- and Anticoagulant Roles

Genevieve. McCluskey, et al., bioRxiv - Immunology 2021

Quote: ... and harvested into EDTA and used to measure plasma hemoglobin (plasma-Hb) and quantify soluble recombinant thrombomodulin (sTM-ELISA, ab209880, Abcam) and thrombin-antithrombin (TAT-ELISA ...

SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors

Chao Gao, et al., bioRxiv - Biochemistry 2020

Quote: ... HRP-labeled goat anti-human IgG was used at 1:1,000 dilution and the color development was with TMB ELISA Substrate (Abcam, ab171523) The experiment was performed in duplicate and the background subtraction was done by subtracting the corresponding binding signals obtained with BSA-immobilized wells ...

Got Milk? Maternal immune activation during the mid-lactational period affects nutritional milk quality and adolescent offspring sensory processing in male and female rats

Holly DeRosa, Hieu Tran, Amanda C. Kentner, bioRxiv - Neuroscience 2022

Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).

Stable High-Concentration Monoclonal Antibody Formulations Enabled by an Amphiphilic Copolymer Excipient

John H. Klich, et al., bioRxiv - Bioengineering 2022

Quote: ... plates were washed 10 times and then developed by adding 50 μL of TMB ELISA Substrate (High Sensitivity) (Abcam, ab171523). Development was stopped after 2.5 min by adding 1 N hydrochloric acid ...

Microglia Mediate Early Corticostriatal Synapse Loss and Cognitive Dysfunction in Huntington’s Disease Through Complement-Dependent Mechanisms

D.K. Wilton, et al., bioRxiv - Neuroscience 2021

Quote: CSF (diluted 1/2000 and 1/4000 in sample diluent) samples were employed in an albumin sandwich ELISA (Abcam ab108788). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.

APOE4 affects basal and NMDAR mediated protein synthesis in neurons by perturbing calcium homeostasis

Sarayu Ramakrishna, et al., bioRxiv - Neuroscience 2020

Quote: ... the conditioned media was given a short spin at 1000 rcf for 2 minutes to remove the cell debris and subjected to ELISA (ab108813, Abcam) to estimate the amount of APOE secreted by iPSCs.

A long-acting prolactin to combat lactation insufficiency

Kasia Kready, et al., bioRxiv - Synthetic Biology 2023

Quote: The serum concentration of His-tagged PRL N59D was determined using a quantitative ELISA as follows: 50ul of 0.1ug/mL anti-human PRL antibodies (Abcam; ab244037) were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates for 1hr at room temperature ...

Serum iron variation is circadian-regulated and linked to the harmonic circadian oscillations of erythropoiesis and hepatic Tfrc expression in mice

Cavan Bennett, et al., bioRxiv - Molecular Biology 2023

Quote: Serum iron and liver and spleen non-heme iron was measured as previously described.27 Serum sTfR and hepcidin were measured by ELISA (Abcam and Intrinsic Life Sciences ...

Cholecystokinin from the Rhinal Cortex Facilitates Motor Skill Learning

Hao Li, et al., bioRxiv - Neuroscience 2022

Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...

The cytoprotective role of antioxidants in mammalian cells under rapidly varying temperature, pressure and UV conditions during stratospheric balloon campaign

Julita Kulbacka, et al., bioRxiv - Cell Biology 2019

Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

P López-Jiménez, et al., bioRxiv - Cell Biology 2022

Quote: ... SYCP3 was detected with either a mouse monoclonal antibody against mouse SYCP3 (Abcam, ab97672) or a rabbit polyclonal antibody recognizing mouse SYCP3 (Abcam ...

Short-term Pre-operative Methionine Restriction Induces Browning of Perivascular Adipose Tissue and Improves Vein Graft Remodeling in Mice

Peter Kip, et al., bioRxiv - Systems Biology 2023

Quote: ... For VSMC+KI-67 double-staining: ACTA2 (mouse anti-mouse, Abcam, ab7817, 1:800) and Ki-67 (rabbit anti-mouse ...

An Innovative Mitochondrial-targeted Gene Therapy for Cancer Treatment

Kai Chen, et al., bioRxiv - Cancer Biology 2024

Quote: Mouse primary antibodies and HRP-conjugated secondary anti-mouse antibodies were obtained from Abcam. TOMM20 and cytochrome c antibodies were purchased from Abcam and Cell Signaling Technology (Danvers ...

Prophase removal of chromosome-associated RNAs facilitates anaphase chromosome segregation

Judith A. Sharp, Wei Wang, Michael D. Blower, bioRxiv - Cell Biology 2019

Quote: ... mouse α-GFP (Abcam, ab1218); mouse α-Aurora-B (Abcam ...

Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization

Ellis L. Ryan, et al., bioRxiv - Cell Biology 2020

Quote: ... mouse anti-TACC3 (ab56595, Abcam), mouse anti-GTSE1 (H00051512-B01P ...

Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy

Rocio Bengoechea, et al., bioRxiv - Cell Biology 2020

Quote: ... anti-mouse Pgk1 (Abcam, 113687), anti-rabbit GAPDH (Cell Signaling ...

Coding variants identified in diabetic patients alter PICK1 BAR domain function in insulin granule biogenesis

Rita C. Andersen, et al., bioRxiv - Cell Biology 2020

Quote: ... mouse-anti proinsulin (Abcam, ab82698) (1:1000) ...