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Citations for abcam :
1 - 50 of 1503 citations for LAG 3 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 3 ml of the adjuvant goat IgG anti-human IgM Fc (5 ng/ml) (by ABCAM, Cambridge, UK) was dissolved in human albumin and phosphate-buffered normal saline added to the remainder of the sediments and packaged in 1 ml vials of injectable solution with labels (See Supplement ...
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bioRxiv - Cell Biology 2024Quote: HEK293 cells expressing human ACVR1-GFP were stained with Lysopainter (Abcam, ab176829) and Hoechst (ThermoScientific ...
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bioRxiv - Bioengineering 2019Quote: Extracellular domain Fc fusions for human CTLA4 (CTLA4-Fc) and ErbB2 (ErbB2-Fc) were purchased from Abcam (ab180054 and ab168896). These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... CoMiX-Fc were detected with a goat anti-human IgG Fc HRP pAb (Abcam, #ab97225), whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... On DIV14 cells were treated with human Fc (Abcam #90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
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bioRxiv - Immunology 2020Quote: ... HRP conjugated goat anti-human IgG Fc was from Abcam, Cambridge ...
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bioRxiv - Microbiology 2023Quote: The Fc portion of human Immunoglobulin G (1 mg, Abcam) was labelled with biotin (Thermo Scientific EZ-Link Sulfo-NHS-Biotin ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were treated with purified Fc from human IgG (Abcam #ab90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
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bioRxiv - Cell Biology 2021Quote: ... bound IRE1α LD-Fc was transferred after washes to a PVDF membrane and detected with anti-human Fc antibody (ab977225, Abcam), ECL solution (Thermo-Fisher ...
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bioRxiv - Immunology 2024Quote: ... 100 ng/well goat anti-human IgG Fc (Abcam, Cambridge, UK, #ab97221) or rabbit anti-His (Bethyl ...
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bioRxiv - Bioengineering 2021Quote: ... anti-human cleaved Caspase 3 (Abcam #ab2302). Biotinylated lotus tetranoglobus lectin was purchased from Fisher Scientific (#NC0370187 ...
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bioRxiv - Immunology 2021Quote: ... the antigens and a capture anti-human IgE (Mouse anti-Human IgE Fc) (ref. ab99834, Abcam PLC, Cambridge, UK) were coupled to magnetic MagPlex® 6.5μm COOH-microspheres (Luminex Corporation ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera) (ab272105, Abcam) was used as positive control (loaded 2.4 ug/lane) ...
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bioRxiv - Neuroscience 2020Quote: ... ephrin-A5 conjugated to human FC (ephrinA5-hFC, R&D) or hFC control (R&D) was pre-clustered with goat anti- human antibody (Abcam) for one hour at 37°C before being added to transfected HEK cells (5 μg /ml ...
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bioRxiv - Immunology 2024Quote: ... CoMiX-Fc molecules were revealed with goat anti-human IgG AF647-conjugated antibody (Abcam), which cross-reacts with mouse IgGs ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with PE-labeled goat anti-human IgG-Fc antibody (1:5000; Abcam) for 30 min and analyzed by flow cytometry.
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bioRxiv - Microbiology 2022Quote: ... cells were dissociated and incubated with recombinant human ACE2-Fc(IgG) (1 mg/ml) (Abcam ab273687) at a dilution of 1:32000 for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and directly incubated with the secondary antibody using Goat Anti-Human IgG Fc (HRP) preadsorbed (Abcam). After washing five times with Tris-buffered saline and Tween 20 ...
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bioRxiv - Bioengineering 2021Quote: ... anti-human cleaved Caspase 3 was purchased from Abcam (ab2302). Primary antibodies were detected with peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... after twice washes then PE-labeled goat anti-human IgG-Fc antibody was added (1:5,000; Abcam) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies included: (1) goat anti-human IgG Fc (HRP) pre-adsorbed (catalog no. ab98624; Abcam); (2 ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were washed again with PBSt and incubated with an anti-human Fc HRP-conjugated antibody (ab977225, Abcam). Development was performed with TMB solution and terminated with Stop solution (Biolegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were then analyzed for galectin 3 content using a human galectin 3 ELISA kit (Abcam, ab 188394) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and C3 levels by Human Complement 3 ELISA kit (Abcam, Cambridge, MA) as per manufacturer instructions.
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bioRxiv - Cancer Biology 2019Quote: ... or Fc-iNKG2D.AF and bound MicAbody detected with an HRP-conjugated mouse-anti-human kappa chain antibody (Abcam #ab79115). Affinity of both monomeric and antibody-fused ULBP2 variants was also determined by Octet analysis as described above.
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Bioengineering 2021Quote: ... and 14 (n = 3) using a Human Albumin ELISA Kit (Abcam; Boston, MA. ab108788) and Urea Assay Kit (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... About 3 µL of polyclonal anti-human α-tubulin Ab (0.3 mg/mL, Abcam) was then added into 150 µL P ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Microbiology 2023Quote: ... and (3) goat anti-human IgM mu chain (HRP) pre-adsorbed (catalog no. ab98549; Abcam). After washing away the unbound secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Fc-CD19-FITC (abcam, ab246020), anti-TIM-3-PE (BD ...
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bioRxiv - Cell Biology 2020Quote: ... SLC31A1/CTR1 (Abcam, ab129067 WB/IF/FC). Rabbit polyclonal antibodies raised against VPS35 (Abcam 97545 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and goat anti-rabbit IgG Fc (Abcam) were amine-coupled to CM3 chips and human and rabbit mAbs were captured to an average density of 320 ± 1.5 RU (s.e.m) ...
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bioRxiv - Cancer Biology 2019Quote: ... cultured osteoblasts or osteoclasts were treated with 25 ng/ml recombinant full-length human MMP-3 (Abcam, ab96555) in place of conditioned medium or in combination with conditioned medium.
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bioRxiv - Cell Biology 2019Quote: ... The primary antibody was diluted with 3% BSA-TBST: mouse-anti-human CXCR7 antibody (ab72100; Abcam; 1:500), rabbit-anti-human CXCR4 antibody (ab2090 ...
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bioRxiv - Neuroscience 2022Quote: Levels of NT3 protein in the serum were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). As the viral vector encoding the human prepro-NT3 gene is identical to mature rat NT3 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 3 times with PBST and then incubated with a goat anti-human HRP (abcam ab7153) secondary ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody dilutions were prepared in 3% NGS in 1XPBS as follows: mouse anti-human vinculin (VIN-54, Abcam) at 1:200 and phalloidin conjugated with Alexa Fluor 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: Levels of NT3 protein in serum and muscles were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). In this study the viral vector encoded the human prepro-NT3 gene ...
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bioRxiv - Microbiology 2021Quote: ... Protein binding between FXa-Fc or Fc and S protein was detected by immunoblotting using an anti-S protein antibody (ab272504, Abcam).
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bioRxiv - Immunology 2020Quote: ... and goat anti-Rabbit IgG Fc (Abcam, USA) was amine coupled to CM3 surface ...
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bioRxiv - Bioengineering 2023Quote: ... EPO receptor protein (Fc chimera active; Abcam, USA) was immobilized on Series S Sensor Chips CM5 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... goat anti-rat IgG Fc 488 (ab97089 abcam), goat anti-mouse IgG Fc Alexa Fluor™ 680 (115625071 Jackson Immuno Research).
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).