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Citations for abcam :
151 - 200 of 1396 citations for Herpes Simplex Virus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... Deparaffinized and rehydrated 5-μm FFPE sections were subjected to antigen heat retrieval using citrate buffer (abcam, ab208572), in a pre-heated steam bath (100°C ...
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bioRxiv - Neuroscience 2020Quote: ... antigen retrieval and antibody staining (anti-PER2, Alpha Diagnostic, cat. PER21-A, 1:200 dilution; anti-GFAP, Abcam, cat ...
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bioRxiv - Cell Biology 2023Quote: ... Antigen unmasking was performed by heating slides submerged in 1X Citrate buffer (pH 6.0) (cat. no. ab64214, Abcam) in a microwave for 1 minute at 100% power ...
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bioRxiv - Immunology 2020Quote: ... Virus replication was detected with mouse mAb RSV5H5 anti-RSV M2-1 (ab94805, Abcam, Cambridge, MA, USA) followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05 ...
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bioRxiv - Microbiology 2019Quote: ... Heat-induced antigen retrieval in citrate buffer (pH 6.0) was performed prior to incubation with primary antibodies: anti-histone H3 (Abcam); anti-Fibrinogen (Thermofisher)) ...
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bioRxiv - Microbiology 2020Quote: ... and/or primary antibody to O157-antigen (1:2000 anti E. coli O157:H7 goat polyclonal antibody, Abcam ab30521) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry was performed on formalin-fixed paraffin embedded lungs following Tris-EDTA pH 9.0 antigen retrieval with an antibody that recognizes CXCL5/6 (Abcam). Routine hematoxylin and eosin (H&E ...
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bioRxiv - Immunology 2021Quote: ... the antigens and a capture anti-human IgE (Mouse anti-Human IgE Fc) (ref. ab99834, Abcam PLC, Cambridge, UK) were coupled to magnetic MagPlex® 6.5μm COOH-microspheres (Luminex Corporation ...
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bioRxiv - Genomics 2020Quote: ... An amount of 0.1% trypsin (A100458-0050, Diamond) was used to recover antigen and 20% goat serum (ab7481, Abcam) was used for blocking ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... incubation with the following primary antibodies was performed following routine heat antigen retrieval (10 mM sodium citrate buffer, pH 6.0): anti-cleaved PARP (Abcam, E51 ...
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinized and rehydrated slides were submerged in 1X antigen retrieval solution (100X Tris-EDTA Buffer, pH 9.0, ab93684, Abcam) and placed in a tissue steamer (IHC-Tek Epitope Retrieval Steamer Set ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... the HIV-1 p24 antigen content in viral stock were quantified using the HIV1 p24 ELISA kit (Abcam, ab218268), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with human TruStain FcX and staining with anti-influenza A virus nucleoprotein antibody (431; Abcam) in 0.1% Triton X-100 solution ...
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bioRxiv - Neuroscience 2021Quote: ... Free–floating slices of cryosectioned (50 μm–thick) brains were subjected to antigen retrieval for 10 minutes at 37 °C using Pepsin Reagent (Abcam). This step was followed by 30–minute incubation with aqueous solutions of 5 % normal donkey serum (NDS ...
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bioRxiv - Cancer Biology 2021Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Immunology 2021Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Developmental Biology 2023Quote: 12μm thick cryosections of organoids were permeabilized in 0.1% Triton/PBS before incubation with primary antibodies at 1/200 dilution using appropriate serum as blocking buffer without antigen retrieval step: SOX2 (rabbit, ab92494 Abcam), TBR2 (sheep ...
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bioRxiv - Immunology 2023Quote: ... Tissues stained with CD163 were then subject to antigen retrieval using sodium citrate pH6 and incubated overnight at 4°C with rabbit anti-IBA1 (ab178846, abcam), followed by anti-rabbit AF488 secondary antibody or with rabbit anti-IBA1 AF647 (ab225261 ...
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bioRxiv - Pathology 2023Quote: ... Slides were then subjected to heat-mediated antigen retrieval by placing slides in boiling Citrate buffer with a pH of 6.0 (Abcam, ab93678) and steamed for 30 minutes (100°C) ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
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bioRxiv - Immunology 2024Quote: ... heat-induced epitope retrieval was performed by boiling the samples in a steamer for 30 minutes in antigen unmasking solution (Abcam, antigen retrieval buffer ...
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bioRxiv - Microbiology 2023Quote: ... mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1) p24 clone [39/5.4A] (#ab9071, Abcam, dilution 1:500). Mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 µM Pitstop-2 (Abcam), hypotonic medium (80% of medium was replaced with water ...
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bioRxiv - Synthetic Biology 2021Quote: ... The remaining antigens were detected with the following antibodies: GD2 was detected with mouse anti-ganglioside GD2 antibody (1:1,000; Abcam; Cat # ab68456); LeY was detected with mouse anti-Lewis Y antibody clone H18A (1:1,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and virus replication was quantitated by measuring the p24 antigen released in culture supernatants at different time points using HIV-1 p24 SimpleStep ELISA kit® (Abcam). Serial dilutions of HIV-1 p24 protein (strain 92418 ...
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bioRxiv - Neuroscience 2020Quote: ... 115-007-003) was used for antigen blocking before overnight incubation at 4 °C with rhodopsin 4D2 (1:500, Abcam, ab98887). Slides were washed and incubated with goat anti-mouse 488 (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... OCHRe Oxford Radcliffe Biobank (ORB)] were deparaffinized and each section was pre-treated using heat mediated antigen epitope retrieval and stained with anti-CD14 (Abcam, AB183322) and Anti-MRC1/CD206 (Atlas Antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... The slides then underwent heat mediated antigen retrieval followed by incubation with primary antibody - anti-Collagen 1 antibody-3G3- (Abcam - ab88147) and the secondary antibody - AffiniPure Goat Anti-Mouse IgG (Jackson Immunoresearch Lab Inc - Code - 115005003) ...
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bioRxiv - Immunology 2020Quote: ... Antigen-coupled beads were validated by incubating them with serial dilutions of anti-histidine-Biotin antibody for antigens with a histidine-tag (Abcam, ab27025). To choose an appropriate coupling concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... using the Ventana Discovery Ultra antigen retrieval CC1 (catalogue #950-500) for 64 minutes followed by the application of anti-PHOX2B antibody (Abcam, ab183741) at a 1:100 dilution for 60 minutes ...
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bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Physiology 2020Quote: ... Anti-Influenza A Virus Nucleoprotein (NP; NR-43899, BEI Resources, Manassas, VA) or Anti-Thrombospondin 1 (ab85762, abcam, Cambridge, UK), with secondary antibody AlexaFluor 594 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The culture supernatants containing pseudotyped viruses particles were either used immediately or flash frozen in aliquots and stored at 80°C until use after being concentrated using a PEG virus precipitation kit (Abcam). Incorporation of the SARS-CoV-2 S protein into the SARS-CoV-2pp was confirmed using Western Blot analysis with anti-SARS-CoV-2 S1 chimeric monoclonal antibody with combined constant domains of the human IgG1 molecule and mouse variable regions (40150-D001 Sinobiological ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Microbiology 2023Quote: ... the cells were then incubated with the following primary antibodies in 0.1% BSA solution (1X PBS) overnight in the dark at 4 °C: mouse monoclonal (1:1000) Respiratory Syncytial Virus (F-protein) (Abcam), rabbit monoclonal (1:1000 ...
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bioRxiv - Microbiology 2023Quote: The main primary antibodies used for gel analysis of virus-infected cells were a commercial HSV-1 VP16 antibody (Abcam) and a rabbit polyclonal antibody against the full-length PRV VP16 that was ordered from Genscript ...
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bioRxiv - Systems Biology 2023Quote: ... These were followed by subsequent staining with 50 μl of primary antibody (Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193, Abcam), 1:500 dilution ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... rehydrated through graded alcohols before performing heat mediated antigen retrieval using a water bath at 80°C for 20 min with citrate buffer at pH6 (Abcam, Cambridge, UK). Endogenous peroxidase was blocked with 3% hydrogen peroxide (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... The function of Y-27632 was confirmed by western blotting of the following antigens: NOTCH1 (ab87982) and Integrin α6 (ab181551) (Abcam, Cambridge, UK).
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bioRxiv - Pathology 2020Quote: ... We used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich and proliferating cell nuclear antigen (PCNA, clone PC10, Abcam) for detecting mitotic spindles and proliferating cells ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... incubated for 20min in a pressure cooker (110-120°C, high pressure) for antigen retrieval and then with antibodies against CD42b (cat no. ab183345, Abcam, Cambridge, UK) or/and GATA1 (cat no ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed with acetone:methanol (40:60) solution and immunostaining was performed using an anti-Vaccinia Virus antibody (1:4000 dilution, #ab35219 Abcam, Berlin, Germany), which was detected with a peroxidase-conjugated anti-rabbit secondary antibody (1:1,000 ...