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351 - 400 of 1017 citations for HSP40 DNAJB1 Human His since 2019

• Caught in the Act: targeted mutagenesis of the herpesvirus fusogen central helix captures transition states

  Momei Zhou, et al., bioRxiv - Microbiology 2023

  Quote: ... The Coomassie staining and Western blotting for His-tagged WT gB and gB[H527P] using rabbit anti-His6 antibody (Abcam) was shown in Fig ...
• Structural study of UFL1-UFC1 interaction uncovers the importance of UFL1 N-terminal helix for ufmylation

  Sayanika Banerjee, et al., bioRxiv - Biochemistry 2023

  Quote: ... The samples along with the control were then loaded on 10% SDS-PAGE followed by immunoblot with anti-6x His or anti-Flag antibody (Abcam).
• Acetylation-dependent coupling between G6PD activity and apoptotic signaling

  Fang Wu, et al., bioRxiv - Biochemistry 2023

  Quote: Bacterially expressed and purified His-tagged G6PD (460 ng) was incubated with 10 ng of activated GST-Fyn (Abcam, #ab84696) in kinase buffer (40 mM Tris pH=7.4 ...
• Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal

  Xiaoning wang, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
• SARS-CoV-2 receptor Angiotensin I-Converting Enzyme type 2 (ACE2) is expressed in human pancreatic β-cells and in the human pancreas microvasculature

  Daniela Fignani, et al., bioRxiv - Cell Biology 2020

  Quote: ... the same ACE2 IHC protocol was applied to other 2 primary antibodies anti-Human ACE2: monoclonal rabbit anti-Human ACE2 (cat. Ab108252, Abcam, Cambridge, UK) diluted 1:100 in PBS 1× supplemented with 5% goat serum ...
• Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis

  Marc Cicero Schubert, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Invasion of human glioma cells through corpus callosum was assessed by immunohistochemistry with antibodies against human-specific nestin (abcam, ab6320, 1:500) and/or vimentin (Themo Fisher Scientific ...
• Proton export drives the Warburg Effect

  Shonagh Russell, et al., bioRxiv - Cancer Biology 2021

  Quote: ... rabbit anti-human CA-IX (ab15086, Abcam, Cambridge, MA); rabbit anti-human MCT1 (sc-50324 ...
• Characterization of the SARS-CoV-2 Spike in an Early Prefusion Conformation

  Tingting Li, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Horseradish peroxidase (HRP)-labeled mouse anti-human antibody (Abcam) was used as secondary antibody at 1:5,000 for 30 min ...
• Sensory nerves enhance triple-negative breast cancer migration and metastasis via the axon guidance molecule PlexinB3

  Thanh T Le, et al., bioRxiv - Cancer Biology 2021

  Quote: ... 1/100 anti-human-TRPV1 (ab3487, Abcam, Cambridge, MA), 1/200 anti-tyrosine-hydroxylase (T1299 ...
• Breaking the fibrinolytic speed limit with microwheel co-delivery of tissue plasminogen activator and plasminogen

  Dante Disharoon, et al., bioRxiv - Bioengineering 2021

  Quote: ... Recombinant human tPA was purchased from Abcam (Cambridge, UK).
• G-baToN: a versatile reporter system for cancer cell-stromal cell interactions

  Rui Tang, et al., bioRxiv - Bioengineering 2020

  Quote: ... anti-human mitochondria antibody was purchased from Abcam (ab92824), anti-GAPDH antibody was purchased from Cell Signaling Technology (5174S)
• ALS-FUS mutation affects the activities of HuD/ELAVL4 and FMRP leading to axon phenotypes in motoneurons

  Maria Giovanna Garone, et al., bioRxiv - Neuroscience 2021

  Quote: ... or rabbit monoclonal anti-human IgG antibody (ab109489, Abcam) and left rotating on a wheel for 1 hour at room temperature in NT2 Buffer ...
• A system-view of B. pertussis booster vaccine responses in adults primed with whole-cell vs. acellular vaccine in infancy

  Ricardo da Silva Antunes, et al., bioRxiv - Systems Biology 2020

  Quote: ... anti-human IgG4-PE (clone HP6025, Abcam, Cambridge, UK) or human IgE-PE (clone BE5 ...
• SARS-CoV-2 hijacks neutralizing dimeric IgA for enhanced nasal infection and injury

  Biao Zhou, et al., bioRxiv - Microbiology 2021

  Quote: ... and rabbit anti-human IgA alpha chain antibody (Abcam).
• The secreted acid phosphatase domain-containing GRA44 from Toxoplasma gondii is required for C-myc induction in infected cells

  William J Blakely, et al., bioRxiv - Microbiology 2019

  Quote: ... rabbit anti-human C-myc at 1:1000 (Abcam), mouse anti-gra5 (Biotem ...
• Exacerbation of mitochondrial fission in human CD34⁺ cells halts erythropoiesis and hemoglobin biosynthesis

  Alvaro M. Gonzalez-Ibanez, et al., bioRxiv - Cell Biology 2020

  Quote: ... and Total OXPHOS Human WB Antibody Cocktail (ab110411, Abcam).
• Interferon-γ Increases Sensitivity to Chemotherapy and Provides Immunotherapy Targets in Models of Metastatic Castration-Resistant Prostate Cancer

  Dimitrios Korentzelos, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and mouse anti-human PD-L1 (28-8, Abcam). Secondary antibodies used were goat anti-mouse Alexa Fluor® 488 or goat anti-rabbit Alexa Fluor® 647 (Life Technologies) ...
• Genome-wide CRISPRi screening reveals regulators of Alzheimer’s tau pathology shared between exosomal and vesicle-free tau seeds

  Juan Carlos Polanco, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-human NUSAP1 rabbit polyclonal (1:500; Abcam ab137230), anti-human VPS18 rabbit monoclonal (1:1,000 ...
• Low normal factor V enhances thrombin generation in hemophilia A by a substrate competition mechanism with factor Xa

  Dougald M. Monroe, et al., bioRxiv - Biochemistry 2022

  Quote: ... Human factor V ELISA kit was from abcam (ab137976). Dynabead™ coupling kit was from Life Technologies (14311D) ...
• Hantaan virus replication is promoted via AKT activated mitochondria OXPHOS

  Yuhang Dong, et al., bioRxiv - Pathology 2022

  Quote: ... Total human OXPHOS Antibody Cocktail was purchased from Abcam; IRDye® 680RD Goat anti-Rabbit IgG (H + L ...
• Recombinant annexin A6 promotes membrane repair in a stem cell derived-cardiomyocyte model of dystrophic cardiomyopathy

  Dominic E. Fullenkamp, et al., bioRxiv - Bioengineering 2022

  Quote: ... and human cardiac troponin T ELISA kit (Abcam, ab223860).
• Global Absence and Targeting of Protective Immune States in Severe COVID-19

  Alexis J. Combes, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human IFITM3-AlexaFluor 647 (clone EPR5242; Abcam; ab198573).
• Covalent inhibition of endoplasmic reticulum chaperone GRP78 disconnects the transduction of ER stress signals to inflammation and lipid accumulation in diet-induced obese mice

  Dan Luo, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: Recombinant 6xHis-tagged human GRP78 protein (Abcam, Cambridge, UK) was biotinylated with EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific ...
• Continual reelin signaling by the prime neurogenic niche of the adult brain

  F. Javier Pérez-Martínez, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-human placental alkaline phosphatase (Abcam, 1:50), goat anti-doublecortin (Santa Cruz ...
• Sorting nexin-27 regulates AMPA receptor trafficking through the synaptic adhesion protein LRFN2

  Kirsty J. McMillan, et al., bioRxiv - Molecular Biology 2020

  Quote: ... mouse monoclonal SNX27 (WB for human SNX27, ab77799; Abcam), rabbit polyclonal SNX27 (WB for rat SNX27 ...
• Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia

  Laura Pasetto, et al., bioRxiv - Neuroscience 2021

  Quote: ... or rabbit polyclonal anti-human Ran primary antibody (Abcam) was incubated with 20 μL of anti-rabbit IgG-conjugated Dynabeads for 2h at 4°C in 0.1% BSA/PBS ...
• Limited Environmental Serine Confers Sensitivity to PHGDH Inhibition in Brain Metastasis

  Bryan Ngo, et al., bioRxiv - Cancer Biology 2020

  Quote: ... polyclonal rabbit anti-human Nuclear Mitotic Apparatus Protein (Abcam Cat# 97585 Lot# B115626 RRID ...
• The asthma gut microbiota influences lung inflammation in gnotobiotic mice

  Naomi G. Wilson, et al., bioRxiv - Microbiology 2022

  Quote: ... stained with polyclonal goat anti-human IgA (Abcam #ab96998) or goat anti-mouse IgA (Abcam #ab97104 ...
• PICH function is required for organization of SUMOylated proteins on mitotic chromosomes

  Victoria A. Hassebroek, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-human TopoIIα 1:300 (#Ab 189342, Abcam), Mouse anti-SUMO2/3 (#12F3 ...
• Liver-Chip: Reproducing Human and Cross-Species Toxicities

  Kyung-Jin Jang, et al., bioRxiv - Pharmacology and Toxicology 2019

  Quote: ... and dog models effluent samples (human and rat: Abcam; dog ...
• Conformational dynamics of SARS-CoV-2 trimeric spike glycoprotein in complex with receptor ACE2 revealed by cryo-EM

  Cong Xu, et al., bioRxiv - Molecular Biology 2020

  Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG1 (Abcam, USA) or HRP-conjugated anti-mouse IgG (Sigma ...
• GPR183 regulates interferons and bacterial growth during Mycobacterium tuberculosis infection: interaction with type 2 diabetes and TB disease severity

  Stacey Bartlett, et al., bioRxiv - Microbiology 2020

  Quote: ... and rabbit anti-human GAPDH (1:2500, Abcam 9485) overnight ...
• Human hepatocyte PNPLA3 148M exacerbates rapid non-alcoholic steatohepatitis development in chimeric mice

  Mohammad Kabbani, et al., bioRxiv - Pathology 2020

  Quote: ... polyclonal rabbit anti–Human Nuclear Mitotic Apparatus protein (Abcam Cat# 97585 Lot# GR268490 RRID AB_10680001),27 and unconjugated ...
• PICH promotes SUMOylated TopoisomeraseIIα dissociation from mitotic centromeres for proper chromosome segregation

  Victoria Hassebroek, et al., bioRxiv - Cell Biology 2019

  Quote: ... Mouse anti-human TopoIIα 1:300 (#Ab 189342, Abcam), Mouse anti-SUMO2/3 (#12F3 ...
• Interferon-γ Increases Sensitivity to Chemotherapy and Provides Immunotherapy Targets in Models of Metastatic Castration-Resistant Prostate Cancer

  Dimitrios Korentzelos, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and mouse anti-human PD-L1 (28-8, Abcam). Secondary antibodies used were goat anti-mouse Alexa Fluor® 488 or goat anti-rabbit Alexa Fluor® 647 (Life Technologies) ...
• GZMK^high CD8⁺ T effector memory cells are associated with CD15^high neutrophil abundance in early-stage colorectal tumors and predict poor clinical outcome

  Silvia Tiberti, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit anti-human E-cadherin (Abcam, Ab1416, 1:100), rat anti-human Ki67 (Ebioscience ...
• Niche-derived soluble DLK1 promotes glioma stemness and growth

  Elisa S. Grassi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... or Recombinant Human DLK-1 protein (Abcam Cat# ab151926). For HIF2alpha inhibition ...
• APOL1 variant-expressing endothelial cells exhibit autophagic dysfunction and mitochondrial stress

  Ashira Blazer, et al., bioRxiv - Cell Biology 2020

  Quote: ... and primary antibodies to anti-human SQSTM1/p62 (Abcam) (both raised in rabbit ...
• APOL1 variant-expressing endothelial cells exhibit autophagic dysfunction and mitochondrial stress

  Ashira Blazer, et al., bioRxiv - Cell Biology 2020

  Quote: ... and mouse anti-human tubulin (1 μg/mL) (AbCam) diluted in 5% BSA/TBS-T overnight ...
• CRISPR-based functional genomics in human dendritic cells

  Marco Jost, et al., bioRxiv - Immunology 2020

  Quote: ... and the SimpleStep human IL-10 ELISA kit (abcam), respectively ...
• High-content phenotypic screen to identify small molecule enhancers of Parkin-dependent ubiquitination and mitophagy

  Roberta Tufi, et al., bioRxiv - Cell Biology 2022

  Quote: Human neuroblastoma control SH-SY5Y cell line (Abcam, ab275475), Parkin knockout (PRKN−/− ...
• Graft of cardiac progenitors in a pig model of right ventricular failure triggers myocardial epimorphosis, regeneration and protection of function

  V Lambert, et al., bioRxiv - Developmental Biology 2022

  Quote: ... or anti human mitochondria antibody (anti-CoxIV, abcam ab197491) to detect human progenitor cells ...
• Neuronal pentraxin Nptx2 regulates complement activity in the brain

  Jiechao Zhou, et al., bioRxiv - Neuroscience 2022

  Quote: ... Human Complement Factor B ELISA Kit (ab137973) from Abcam according to the manufacturer’s instructions ...
• Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

  Martin H. Berryer, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Chicken anti-human MAP2 (1:1000, Abcam, ab5392) primary antibodies ...
• Genome-wide CRISPRi screening reveals regulators of Alzheimer’s tau pathology shared between exosomal and vesicle-free tau seeds

  Juan Carlos Polanco, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-human VPS18 rabbit monoclonal (1:1,000; Abcam ab178689), anti-human Tau-13 mouse monoclonal (1:1,000 ...
• PKC-alpha promotes phosphorylation of KRAS suppressing its oncogenic properties

  Tejashree Joglekar, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and Human CXCL1 ELISA Kit (GRO alpha) (abcam #ab190805) were used to detect the indicated chemokines ...
• Mutational order and epistasis determine the consequences of FBXW7 mutations during colorectal cancer evolution

  Dedrick Kok Hong Chan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-human NOTCH1 (1:2500, ab52627, Abcam, USA), and rabbit anti-human GAPDH (1:2500 ...
• Immune Response of Transplanted Kidney Tissues Assembled from Organoid Building Blocks

  Thiago J. Borges, et al., bioRxiv - Bioengineering 2023

  Quote: ... Antibodies used were rabbit polyclonal anti-human CD31 (Abcam). Stained sections were photographed using an Axio Imager M2 microscope (Zeiss ...
• Mutational order and epistasis determine the consequences of FBXW7 mutations during colorectal cancer evolution

  Dedrick Kok Hong Chan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-human CMYC (1:2500, ab32072, Abcam, USA), rabbit anti-human NICD (1:2500 ...
• Robust and Versatile Arrayed Libraries for Human Genome-Wide CRISPR Activation, Deletion and Silencing

  Jiang-An Yin, et al., bioRxiv - Genomics 2023

  Quote: ... 1 µl of Alexa488 anti-human EPCAM (Abcam, ab112067) or Alexa647 anti-mouse/human CD44 (Biolegend ...