Labshake search
Citations for abcam :
151 - 200 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution (1:500 Rabbit anti-GFP Abcam 290; 1:50 Mouse anti-HP1a DSHB, C1A9) and incubated with embryos at 4°C overnight ...
-
bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...
-
bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by immunostaining with primary antibodies (diluted in blocking buffer) directed against NeuN (Abcam, #ab104224; 1:500), FABP7 (EMD Millipore ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were incubated in blocking solution over night at 4 °C (RUNX1 1:200 - ab92336, Abcam). Cells were then washed in PBS-T and incubated with secondary antibodies for 1 hour at room temperature (donkey α-rabbit 1:200 - A-11008 - Thermo Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... The following primary antibodies used were diluted in blocking buffer: anti-Argonaute-2 (1:1000) (Abcam #32381), anti-Argonaute-1 (1:1000 ...
-
bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used diluted in blocking buffer– chicken anti-GFP (Abcam ab13970; 1:1000), mouse anti-PDF (DSHB-PDF C7 ...
-
bioRxiv - Neuroscience 2023Quote: ... They were incubated with the primary antibody (prepared in 1% BSA blocking buffer) (Fibrillarin - Abcam Cat#AB4566) overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated in blocking solution over night at 4 °C (RUNX1 1:200 - ab92336, Abcam). Cells were then washed in PBS-T and incubated with secondary antibodies for 1 hour at room temperature (donkey α-rabbit 1:200 - A-11008 - Thermo Scientific) ...
-
bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were then added in blocking buffer: anti-nestin [10C2] 1:200 (mouse monoclonal, Abcam, ab22035); anti-p(Th316)-nestin [a-4] at 1:200 (mouse monoclonal ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1:500 rabbit anti-PARP1 Abcam ab227244; 1:500 rabbit anti-PCNA Abcam ab18197, 1:500 mouse anti-PCNA Abcam ab29 diluted in blocking solution). After antibody incubation ...
-
bioRxiv - Developmental Biology 2022Quote: ... respectively and labelled oocytes were transferred to glycerol mounting medium (Abcam, AB188804) prior to imaging.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with appropriate primary antibodies prepared in blocking buffer against glucagon (mouse antiglucagon antibody, Cat # ab10988, Abcam; 1:1000 or rabbit anti-glucagon antibody, Cat# ab92517, Abcam; 1:25), Stathmin-2 (goat anti-SCG10 antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH ...
-
bioRxiv - Cancer Biology 2023Quote: ... endogenous peroxidase inactivation with 3% H2O2 was performed on dewaxed specimen followed by blocking with avidin biotin blocking solution (ab64212, Abcam) and 5% BSA in TBST ...
-
bioRxiv - Developmental Biology 2023Quote: ... For anti-PI4KIIIalpha staining, the blocking buffer (for blocking, primary, and secondary steps) was supplemented with 5% Normal Goat Serum (Abcam).
-
bioRxiv - Immunology 2023Quote: ... followed by treatment with C5a peptide (AbCam) at 50 ng/ml for 4 hrs ...
-
bioRxiv - Neuroscience 2021Quote: ... and blocking with 6% goat serum (Abcam, ab7481). Samples were then incubated with antibodies for Cux1 (Santa Cruz ...
-
bioRxiv - Biochemistry 2021Quote: ... After blocking with 5% goat serum (Abcam, ab7481) in PBS for 1 h at 25°C ...
-
bioRxiv - Cell Biology 2020Quote: ... and blocked in 1X blocking solution (Abcam #126587) diluted in 0.2%TritonX-100 in 1X PBS for 1hr at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... we used Avidin/Biotin blocking kit (Abcam, USA) followed by incubation with normal serum (5% Donkey and 5% Goat serum ...
-
bioRxiv - Molecular Biology 2024Quote: ... Blocking was performed in 10% goat serum (Abcam) diluted in permeabilization buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 1 hour at room temperature with a primary antibody against C-peptide (Abcam), followed by 1-hour incubation with corresponding fluorescently labeled secondary antibody ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibody was diluted in blocking buffer at the following dilutions: anti-CD34 (1:100; Abcam #MA1-10202), anti-FAPα (1:200 ...
-
bioRxiv - Cell Biology 2021Quote: ... blocked in odyssey blocking buffer for 1 hour and probed with streptavidin IR800 and anti-galectin 3 (Abcam). Membranes were imaged using a LI-COR Odyssey IR imager.
-
bioRxiv - Developmental Biology 2022Quote: ... Tissues were then incubated in blocking buffer 1 (BB1) consisting of 5% v/v donkey serum (abcam, ab7475), 1% v/v BSA ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight in rabbit anti-c-Fos primary antibody (1:2000 in blocking solution, Abcam, ab190289). Sections were then rinsed in PBS (5 x 5 min ...
-
bioRxiv - Genomics 2023Quote: ... Primary antibody incubation was done in blocking buffer for 1 h at rt (anti PR Ser294Phospho, ab61785, Abcam). Primary antibody was washed 3 times in wash buffer (0.2% BSA ...
-
bioRxiv - Molecular Biology 2022Quote: ... Slides were gently washed in phosphate buffered saline (PBS) prior to blocking with the blocking reagent from the mouse-on-mouse polymer IHC Kit (Abcam ab269452). The tissues were then incubated overnight with the primary antibody at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
-
bioRxiv - Bioengineering 2023Quote: ... After completion of blocking the membrane was incubated in primary antibody overnight on a shaker (Primary antibodies: Syntenin-1 (Abcam, ab133267 1:2000); CD81 (Santa Cruz ...
-
bioRxiv - Cell Biology 2019Quote: PKA activity was measured using a PKA kinase activity assay kit (Abcam, Cambridge, UK), according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... The kinase substrates containing thiophosphate esters were then detected by α-Thiop RabMAb (Abcam).
-
bioRxiv - Cell Biology 2022Quote: ... PKA activity was measured using an ELISA kit (PKA kinase activity kit, Abcam 139435) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... LRRK2 kinase activity was inhibited pre-treating cells with 0.5μM MLi-2 (ab254528, Abcam) for 90 minutes.
-
bioRxiv - Genomics 2019Quote: ... followed by elution twice with 100 μl 2.5 mM CTD peptide (synthesized by Peptide Specialty Laboratories, Heidelberg, Germany; identical to Abcam ab17564) for 30 min ...
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
-
Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... lamin A/C (1:100 in PBS; following blocking with NGS, 2 hr at 37°C) (Abcam; ab108595, USA), or nucleostemin (1:250 in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were incubated with primary antibodies (in blocking solution) using the following dilutions: Chx10 1:100 (abcam, Cambridge, UK), Dmrt3 1:5000 (custom made (Andersson et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in primary antibody diluted in blocking solution containing Triton-X (0.4%): chicken anti-YFP (1:100, Abcam), rabbit anti-NG2 (1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
-
bioRxiv - Cell Biology 2022Quote: ... diluted in blocking buffer for one hour at RT: donkey anti-rat Alexa Fluor® 647 (1:250; abcam); donkey anti-goat Alexa Fluor® 568 (1 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections were incubated on a shaker for 48 h at 4 °C with primary antibodies in the blocking buffer containing 0.1% Triton X-100 at the following dilutions: rabbit anti-TH (1:1000, Abcam), chicken anti TUJ1 (1:1000 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sections were then stained overnight in the same blocking buffer with primary antibodies against GFP (1:2000 Abcam 13970), YAP (1:200 Cell Signaling Technologies 14074S) ...