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Citations for abcam :
251 - 300 of 302 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Levels of GSH and GSSG were measured using the GSH/GSSG Ratio Detection Assay Kit II (Abcam catalog # ab205811). NADPH and NADP+ were measured using the NADP/NADPH assay kit (Abcam catalog # 176724).
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bioRxiv - Immunology 2022Quote: ... according to the manufactures instructions before intracellular staining with monoclonal antibodies specific for hexokinase II or lactate dehydrogenase (Abcam).
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bioRxiv - Molecular Biology 2022Quote: ... Diluted nuclear extracts were incubated with anti-RNA polymerase II CTD repeat YSPTSPS antibody (8WG16)-ChIP grade (Abcam, ab817) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558, Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: Skeletal muscle GSH and GSSG was measured using the fluorometric GSH/GSSG Ratio Detection Assay Kit II (Abcam 205811). Briefly ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4°C with 0.1 µg of anti-RNA polymerase II monoclonal antibodies (Abcam ab817 - 8WG16-) and 5 µl of protein A ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: Immunohistochemistry was performed to localise expression of collagen type II (Col2) (Thermo, Mouse MC) and SOX9 (Abcam, Rabbit PC). Histology slides were dewaxed and rehydrated ...
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bioRxiv - Immunology 2020Quote: ... HT29 (human colorectal adenocarcinoma grade II) whole cell lysate was used as a positive control for TMPRSS2 (Abcam, ab3952). Full length predicated at 54 kDa and the cleavage fragment at 25 ...
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bioRxiv - Cancer Biology 2019Quote: ... For ChIP in RNAPII pausing experiments we used anti-RNA polymerase II CTD repeat YSPTSPS antibody (8WG16) (ab817, Abcam) and mouse IgG isotype control (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... mouse OXPHOS cocktail (anti-ATP5A, anti-UQCRC2, anti-SDHB, anti-COX II and anti-NDUFB8) (Abcam #ab110411, 1:1000), rabbit anti-AIF (Cell Signaling Technologies #5318 ...
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bioRxiv - Zoology 2023Quote: ... The membranes were incubated with primary antibodies against anti-collagen-II (1:1000 dilution, ab188570, abcam Co., Cambridge, UK), anti-Bcl-2 antibody (1:500 dilution ...
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bioRxiv - Immunology 2021Quote: ... for one hour and incubated with primary antibody mix (anti-ki67, anti-KT10 antibody & anti-KT16 antibody, all Abcam) for one hour at room temperature and then overnight ...
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bioRxiv - Bioengineering 2020Quote: ... and incubated overnight with primary antibodies at 4 °C (Col I antibody: ab6308, 1:500, Abcam; Col II antibody, 1:10, Quartett Immunodiagnostika; ALP antibody, ab95462, Abcam). Afterwards ...
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bioRxiv - Bioengineering 2019Quote: ... both rabbit anti-human (Rockland 600-401-104, 1:200, for collagen II, Abcam ab138492, 1:1500 for collagen I) were applied in 1 % NGS ...
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bioRxiv - Cell Biology 2022Quote: ... The pre-cleared supernatants were divided into two parts and incubated with 40 μL Dynabeads pre-bound with antibodies for 3.5 μg NONO or RNA polymerase II phosphorated at Serine 2 (Abcam, ab5095) and normal mouse IgG overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used were anti-RP II mouse monoclonal antibody antibody (sc-56767 of Santacruz Biotechnology) and rabbit polyclonal anti-RNase H1 (ab229078 of abcam). The secondary antibodies IRDye®800CW or IRDye®680RD (LI-COR Biosciences ...
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bioRxiv - Bioengineering 2019Quote: ... The same protocol was applied for Strep-tag detection using a 1:1000 dilution of the anti-Strep-tag II antibody (ab76949, abcam) and a 1:10000 dilution of ‘Immun-Star Goat Anti-rabbit-HRP conjugate’ (170-5046 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 3 times in 0.1% Tween-20 in TBS and incubated overnight at 4C with antibodies to: MHC II (Abcam ab55152), TZAP (Abnova H0003-B01P) ...
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bioRxiv - Bioengineering 2022Quote: ... BD FACS Canto II was used to analyze VCAM-1 positive cells using FTIC- anti-VCAM-1 antibody (CC# ab24853; Abcam) and the number of positive cells were quantified using with FlowJo software (Tree Star) ...
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bioRxiv - Developmental Biology 2022Quote: ... brains were dissected and homogenized before treatment with 1% PFA (protocol adapted from 56) and performed as previously described 57 using 4mg of RNA Polymerase II antibody (ab817, Abcam) per sample ...
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bioRxiv - Microbiology 2023Quote: ... and 12.5 uL (12.5 ug) of MHC class II antibodies in a 3:1:1 mixture of TAL-1B5 (Abcam, ab20181), EPR11226 (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect muscle-specific type II myosin heavy chain (MHC) for 30min at room temperature with secondary antibody Dylight588 (Abcam) and Cy3 secondary detection (Jackson ImmunoResearch ...
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bioRxiv - Genomics 2023Quote: ... We stained polytene chromosomes with rabbit anti-CLAMP (1:1000, SDIX) and mouse anti-RNA pol II (1:500, ab817, Abcam) antibodies ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C with a mix of primary antibodies: chicken anti-GFAP (1:500, Abcam ab4774) and rabbit anti-GFP (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP for RNA polymerase II was performed according the iDeal ChIP-Seq Kit instructions using the anti-Pol2 antibody (Abcam, ab5408). Immunoprecipitated DNA was bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo) ...
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bioRxiv - Cell Biology 2022Quote: ... Epitope detection was conducted with a primary monoclonal mouse antibody against NONO at 1:500 dilution in PBST and a polyclonal rabbit antibody against RNA Pol II phosphorated at Serine 2 (Abcam, ab5095) at 1:500 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... overnight incubation with primary antibody for collagen type I and collagen type II at 4 °C (Ms mAb to collagen I, ab6308, 1:500, Abcam, UK) (collagen type II Ms 6B3 ...
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bioRxiv - Biophysics 2020Quote: ... and subsequently incubated for 1 h at room temperature with rabbit polyclonal antibody directed against RNA polymerase II phosphoS2 (Abcam - ab5095), followed by an incubation with fluorescent Alexa-568 anti-rabbit secondary antibody (ThermoFischer Scientific A11011) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg/ml for inhibition of both Pol I and Pol II transcription, 10 ng/ml for inhibiting only Pol I transcription, Abcam, ab141058), triptolide (TPL ...
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bioRxiv - Biochemistry 2020Quote: ... ab191292), MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (anti-ATP5a, anti-complex III, anti-complex II) (MS604) (all from Abcam), monoclonal anti-catalase (D5N7V ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked in 5% horse serum then incubated overnight with anti-CENP-C antibody (1:200, gift from W. Earnshaw) and anti-RNA pol II (1:1000, Abcam Ab24758) antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... guinea pig anti-Dorsal (a kind gift of Christos Samakovlis) and rabbit anti-RNA Polymerase II CTD repeat YSPTSPS (phosphor S5) (5SerP) (Abcam, ab5131) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were incubated in 100 µl PEMBAL with anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) (Abcam, ab252855, 1:1000) at 4°C overnight ...
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bioRxiv - Pathology 2024Quote: ... activity of SDH/mitochondrial ETC complex II (CII) was assessed on isolated mitochondria from gastrocnemius muscles according to the manufacturer directions (Abcam, ab228560). Mitochondria were isolated as performed by us previously34.
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bioRxiv - Developmental Biology 2024Quote: ... Erythrocytes were incubated for 30 min at room temperature in blocking buffer and stained directly on slides with anti-RNAP II Phosho-Ser2 (Abcam #5095) in the blocking buffer (1X PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... The fragmented chromatin was incubated with pre-bounded Dynabeads (A and G mix) -Invitrogen 10004D/ 10002D using H3K27ac antibody (Abcam, ab4729) and H3K4me2 antibody (Millipore ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Genetics 2022Quote: ChIP-seq data of RNA Pol II in wild-type Col-0 and the epigenetic mutants were obtained as previously described23 and using anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2, ab5095; Abcam, Cambridge, UK) and anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5 ...
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Developmental Biology 2024Quote: ... the tissue sections were incubated with primary antibodies including α-TECTA produced in rabbit (1:1000) or mouse α–Col II (1:100; ab150771, Abcam)) at 4°C overnight ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: Plasma standards of monomeric and polymeric Z α1-antitrypsin were prepared and assayed with an antibody mix comprising a 1:160 dilution of rabbit polyclonal anti-α1-antitrypsin (Abcam product 9373), 0.23μg/ml Terbium anti-mouse ...
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bioRxiv - Biochemistry 2021Quote: TEM assays were performed for samples from co-IP assays using antibodies to RNA Pol II (CTD4H8) or FLI1 (Abcam, cat. no. 15289). Carbon Film 300 Mesh Copper grids (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2019Quote: ... For the localization of total fraction of RNAPII we used mouse 8WG16 antibody (anti-RNA polymerase II CTD repeat YSPTSPS, Abcam, diluted 1:200), according to protocols provided by Kołowerzo-Lubnau et al64 ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin samples were incubated overnight at a low temperature with 10 µg ChIP-grade anti-RNA polymerase II (ab26721; Abcam, Boston, MA, USA) and anti-VDR (sc-1008x ...
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bioRxiv - Immunology 2023Quote: ... then incubated overnight with a mix of primary antibodies, containing rabbit anti-CFTR (Alomone, 16 μg/ml) and chicken anti-GFP (Abcam, 35 μg/ml) at 4C ...
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...