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Citations for abcam :
501 - 550 of 3925 citations for Cow Four And A Half LIM Domains Protein 2 FHL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a four-hour incubation period in donkey anti-goat Alexa Fluor 488 (1:500, Abcam Cat# AB150105, RRID: AB_2732856) and Alexa Fluor 647-conjugated streptavidin (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed with 0.3% PBSTx four times for 15 min per wash and incubated in Alexa Fluor 488 Goat Anti-Mouse IgG H&L (Abcam, ab150117) at a 1:1000 dilution at room temperature (RT ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Microbiology 2020Quote: ... goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer; ab97240, Abcam, Cambridge, UK), or goat-anti-mouse IgG2a (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... Concentrations of total immunoglobulins (IgA and IgG) were evaluated by ELISA (Abcam, Paris, France) to validate the method of protein extraction from stools (Figure 1).
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bioRxiv - Physiology 2023Quote: Twenty microliters of supernatant from liver homogenates were diluted with TGF-β1 ELISA (Abcam) assay buffer (180 ul) ...
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bioRxiv - Biochemistry 2023Quote: Soluble CD95L was dosed by ELISA (#ab45892) following the manufacturer’s instructions (Abcam, MA, USA).
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bioRxiv - Immunology 2021Quote: ... Labelling of recombinant proteins was carried out using APC and PE conjugation lightning-link kits (Abcam). When using biotinylated antibodies ...
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bioRxiv - Developmental Biology 2022Quote: O-propargyl-puromycin (OP-puro) labeling was performed with a Protein Synthesis Assay Kit (ab235634; Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasma membranes were separated from intracellular membrane using the Plasma Membrane Protein Extraction Kit (Abcam ab65400)[33] ...
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bioRxiv - Neuroscience 2024Quote: ... The activity of LOX protein was measured using an LOX activity assay kit (Abcam, Cat. ab112139) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... COX-2 specific enzymatic activity was measured using the cyclooxygenase activity assay kit (abcam ab204699) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 µM Pitstop-2 (Abcam), hypotonic medium (80% of medium was replaced with water ...
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bioRxiv - Neuroscience 2019Quote: Corning High Binding 96-well ELISA plates were coated with MJFR1 (Abcam, Cambridge, United Kingdom) (0.25 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected and C4BPB was quantified via ELISA according to manufacturer’s instructions (Abcam, ab222866).
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bioRxiv - Cell Biology 2021Quote: ... the concentration of C3/C3b in culture supernatants were analysed by ELISA assay (Abcam, UK). Samples were loaded undiluted along with standards and controls in 96 well-plates coated with specific C3b antibody and the assay was performed according to the manifacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Levels of prolactin were determined in undiluted plasma samples using an ELISA (Abcam, Cat.# ab272780). Whole hypothalamus was dissected from offspring and dams ...
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bioRxiv - Microbiology 2020Quote: ... Capsid p24 was measured in cell extracts and supernatants using SimpleStep ELISA (ABCAM, Cambridge, UK) and ADVIA Centaur HIV Ag/Ab Combo ELISA (Siemens Healthcare Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... Granzymes A and B secreted into the cell media were measured by ELISA (Abcam, Invitrogen). IELs were analyzed for CD107a externalization through flow cytometry ...
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bioRxiv - Biochemistry 2023Quote: ... Human amylase was detected in CVLs using an ELISA for human pancreatic amylase (Abcam ab137969) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Bioengineering 2023Quote: Testosterone concentrations in sera were measured with an enzyme-linked immunosorbent assay (ELISA; Abcam ab174569) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... Isolation and protein extraction of mitochondria was performed by Mitochondria Isolation Kit for Cultured Cells (ab110170. abcam). Proteins were separated by Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: Protein lysates were used to conduct lipid peroxidation assay using colorimetry (Lipid Peroxidation, MDA Assay kit (abcam) following manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: ... mtDNA was extracted from 2×107 of Mito+ RBCs with the Mitochondrial DNA Isolation Kit (Abcam) and quantified with Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Pathology 2019Quote: ... Soluble proteins (20 μg) were used for Western blotting with primary antibodies against AKT1/2/3 (rabbit monoclonal antibody; EPR16798 Abcam; 1:10,000), mTOR (rabbit monoclonal antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Cell number was estimated either by counting four fields of view per culture in a haemocytometer after trypan blue staining (Abcam protocols, Cambridge, UK), or by fluorescence-activated cell sorting (FACS) ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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bioRxiv - Cell Biology 2021Quote: ... Homocysteine levels in supernatants containing 25 µg of protein were determined using the Homocysteine Assay Fluorometric Kit (Abcam) following manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Endogenous peroxidase and protein blocking steps were performed using reagents provided in the ABC detection kit (Abcam, ab64261). Sections were incubated overnight with primary antibodies at 4°C (Table S2) ...