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Citations for abcam :
301 - 350 of 10000+ citations for Charged Multivesicular Body Protein 2b CHMP2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... proteins (0.8-1 mg) were rotated overnight at 4°C with 5 µg of mouse anti-SGTa antibody (Abcam) or control mouse IgG and protein G Dynabeads (Invitrogen) ...
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bioRxiv - Systems Biology 2020Quote: ... The primary antibodies against the following proteins were used in this study: ATP synthase complex (Abcam, Cambridge, MA, USA), KIF5B (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting nuclear extract was incubated with Dynal protein G beads which were preincubated with 5μg of H3K27me3 or H3 antibody (ab6002 and ab1791, Abcam) respectively at 4°C overnight ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were used as follows: rat anti-glial fibrillary acidic protein (GFAP, 1:100, Abcam, Cambridge, MA, USA) for astrocytes and rat anti-CD11b (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... or mouse antibodies recognizing cellular proteins (anti-EEA1, BD Biosciences, 610457, 1:100; anti-VPS35, Abcam, ab57632, 1:200); rabbit antibodies recognizing cellular proteins (anti-TGN46 ...
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bioRxiv - Molecular Biology 2023Quote: ... In each capillary 0.2mg/mL protein was loaded for analysis with antibodies to dystrophin (Abcam # ab15277, dilution 1:15) as in 26 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in primary antibodies against the first protein of interest and tubulin (Abcam, Cambridge, UK,; 1:5000) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were probed in Can Get Signal Solution1 (Toyobo) using following primary antibodies: anti-Gpx4 (1:10,000, Abcam, ab125066), anti-β-actin (1:20,000 ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Plant Biology 2019Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... 1:1000 dilution in blocking buffer) plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919 ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Plant Biology 2019Quote: ... Protein detection was performed using a monoclonal rabbit anti-VEGF primary antibody (1:1000 dilution, ab52917, Abcam plc, Cambridge, UK) and a goat anti-rabbit HRP secondary antibody (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... 2019) using antibodies against cAMP response element-binding protein (CREB; clone LB9, produced in mouse, #ab178322, AbCam, Cambridge, UK; RRID:AB_2827810), phospho-CREB (Ser133 ...
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bioRxiv - Physiology 2021Quote: ... Blots were incubated overnight in rabbit anti-mouse primary antibody for proteins of interest (Supplemental Table S4) and with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (ab6721, Abcam). Proteins of interest were detected by incubating blots in ClarityTM Western ECL Blotting Substrate (1705061 ...
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bioRxiv - Molecular Biology 2019Quote: ... primary rabbit raised antibodies against pCRKL Y207 (37 kDa) and pCRK Y221 (34 kDa) (another downstream target protein) were procured from Abcam plc ...
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bioRxiv - Neuroscience 2020Quote: ... Dilutions of the antibodies were as follows: chicken anti glial-fibrillary acidic protein (GFAP2; 1:1,1000; Abcam, Cambridge, United Kingdom); mouse anti-microtubule associated protein (MAP2 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections used for non-vascular associated protein visualization were incubated overnight in primary antibodies: rabbit anti-MBP (1:200, Abcam), or rat anti-MBP (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Chromatin immunoprecipitation was performed by incubating the pre-cleared chromatin with 75 μL Dynabeads Protein G bound with 7.5 μg H3K27ac antibody (Abcam ab4729) overnight at 4°C rotating ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S-protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam) 90.
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against S14 phosphorylated Syx (Abcam; ab63571), using the ECL detection system (Pierce Protein Research Products ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against Syx phospho-S14 (Abcam; ab63571). Digitized scans were derived by PhosphorImager (Molecular Dynamics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mCherry was identified based on its auto fluorescent signal while the luciferase protein was immuno-stained with anti-firefly luciferase antibody (Abcam) labeled with Zenon AF647 Mouse Labeling Kit (Life Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were validated for GPC1/5 knockouts via Western analysis following heparinase III digestion using a monoclonal antibody (3G10) direct against the glycosylation attachment site in the core protein structure (370260, abcam) or via sequencing (GPC1 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 2 hours with: (i) chicken polyclonal antibody anti microtubule-associated-protein 2 (MAP-2) (Abcam, #Ab5392) at dilution of 1/1000 in PBS containing 1% fetal calf serum and 0.1% of saponin (this antibody stains specifically cell bodies and neurites ...
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bioRxiv - Cell Biology 2021Quote: ... The co-immunoprecipitated protein complexes were detected using western blotting with the following antibodies: anti-TCFL5 (1:1000, ab188075, Abcam), anti-FXR1 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...