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Citations for abcam :
1 - 50 of 1748 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mitochondrial isolation was performed using Mitochondrial Isolation Kit for Cultured Cells (Abcam; ab110170) following manufacturers’ instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Isolation and protein extraction of mitochondria was performed by Mitochondria Isolation Kit for Cultured Cells (ab110170. abcam). Proteins were separated by Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from cultured cells using mitochondria isolation kit (Abcam, ab110168). Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested via scraping before histone isolation according to manufacturer’s protocol (abcam 113476). Protein gels were then loaded with 8 μg of total protein isolate as quantified via BCA (Pierce 23225 ...
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bioRxiv - Cell Biology 2021Quote: ... A Preadipocyte Isolation Kit (ab196988, Abcam) was used for preadipocyte isolation and purification according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: The Mitochondria Isolation Kit (Abcam ab110170) was used to isolate mitochondria from MCAD silenced and control GSCs ...
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bioRxiv - Cancer Biology 2022Quote: ... and Mitochondrial DNA Isolation Kit (abcam). 1µg of either mitochondrial or nuclear DNA was incubated with 100 ng of StrepANKLE1 in a buffer containing ...
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bioRxiv - Cancer Biology 2023Quote: A mitochondria isolation kit (Abcam, ab110171) was used to prepare the large organelles/debris and intact mitochondria fractions from JJN3 cells ...
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bioRxiv - Cell Biology 2021Quote: ... lysosomes were purified from vehicle or MakA-treated HCT8 cells using Lysosome Isolation Kit (ab234047, Abcam), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Mitochondria Isolation Kit for Tissue (Abcam, ab110168) was used for the separation of mitochondrial and cytosol fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were lysed and mitochondrion was purified according to the manufacturer’s protocol in a mitochondrial isolation kit for cultured cells (Abcam). The activity was determined by following the manufacturer’s protocol with a standard curve.
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bioRxiv - Immunology 2024Quote: ... Mitochondria and cytosolic fractions of each group were isolated utilizing the Mitochondria Isolation Kit for cultured cells (#ab110170, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... both expressed in wheatgerm cell-free systems (Abcam), were measuring through PLK4-dependent phosphate incorporation from gamma-32P-labelled ATP ...
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bioRxiv - Molecular Biology 2022Quote: The Mitochondrial Yeast Isolation kit and protocol (ab178779; Abcam) were used to fractionate yeast cells by differential centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the microsomes expressing the enzymes were purified from the infected Sf9 cells with a Microsome Isolation Kit (Abcam, Cambridge, MA). Microsomes were incubated with an NADPH regeneration system (Promega ...
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bioRxiv - Genomics 2020Quote: ... we extracted samples using the Urine DNA Isolation Kit (Abcam) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... total protein isolation was performed through standard RIPA method (Abcam). From each sample 30-40µg of protein was loaded into a 12% polyacrylamide gel as previously described [86 ...
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bioRxiv - Cancer Biology 2024Quote: ... mtDNA was isolated using the Mitochondrial DNA Isolation Kit (Abcam) followed by the Qiaprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were washed three times with water and then partially lysed with a yeast nuclei isolation kit (Abcam 206997) using the following modified protocol ...
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bioRxiv - Molecular Biology 2021Quote: Nuclear RNA was obtained using the Yeast Nuclei Isolation kit (Abcam) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Membrane samples were obtained by the Plasma Membrane Isolation Kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: We used exosome isolation and analysis kit (cat: ab239682) from Abcam and followed protocol as directed ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondrial fractions were isolated using the mitochondrial isolation kit from Abcam (Toronto ...
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bioRxiv - Neuroscience 2021Quote: after isolation (described above) synaptosomes were stained with PSD-95 (Abcam ab13552) or Synapsin-1 (Millipore #1543 ...
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bioRxiv - Developmental Biology 2019Quote: ... The sedimented mitochondria were washed in isolation buffer supplemented with PI cocktail (Abcam, ab201111), and was used for mitochondrial respiration analysis ...
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bioRxiv - Molecular Biology 2020Quote: Mitochondria were isolated from frozen heart tissue using a Mitochondria isolation kit (ab110168, abcam). Tissue was briefly washed in isolation buffer ...
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bioRxiv - Neuroscience 2019Quote: ... mitochondrial enriched fractions were isolated as indicated in Mitochondria Isolation Kit for Tissue (Ab110168, Abcam).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and liver mitochondria were isolated from with Mitochondria Isolation Kit for Tissue (Abcam, Cambridge, MA).Liver mitochondrial DNA was then extracted from mitochondria using DNeasy Blood & Tissue kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... mtDNA was extracted from 2×107 of Mito+ RBCs with the Mitochondrial DNA Isolation Kit (Abcam) and quantified with Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: Yeast nuclei were prepared with reagents from the Yeast nuclei isolation kit (Abcam 206997, Cambridge, United Kingdom), unless noted otherwise ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Mitochondria were isolated from adult rat brain by differential centrifugation using mitochondria isolation kit for tissue (ab110168, abcam). Western blot for alpha-synuclein was performed using 4–12% Bis-Tris gels (Life Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Following primary antibodies (αTFAP2A, Abcam ab108311; αTFAP2B, Abcam ab221094; αLEF1, Cell Signaling, 2230; αSOX2, R&D Systems, AF2018; αPITX1, Abcam ab70273 ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-HBP (pAb ab167336, Abcam systems), anti-SMAD2/3 (D7G7 ...
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bioRxiv - Immunology 2020Quote: ... cell supernatant was removed and evaluated to determine cytokine levels using the FirePlex®-HT assay system (Abcam, Cambridge, MA, USA; described below). To label mitochondria in live cells for Cell Painting Palette 2 ...
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bioRxiv - Biophysics 2023Quote: ... Primary tubulin antibodies (#T6199 Sigma Aldrich, #302211 Synaptic Systems, Göttingen, Germany, #302203 Synaptic Systems, #ab18251 Abcam, Cambridge, UK) were applied for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... NDUFS2 (Abcam, ab110249; OXPHOS system Complex I).
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bioRxiv - Molecular Biology 2019Quote: ... NDUFB8 (Abcam, ab110242; OXPHOS system Complex I), MT-ND1 (a kind gift of Dr ...
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bioRxiv - Molecular Biology 2019Quote: ... SDHB (Abcam, ab14714; OXPHOS system Complex II), NDUFB8 (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... ATP5A1 (Abcam, ab14748; OXPHOS system Complex IV), SDHB (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... NDUFA9 (Abcam ab14713; OXPHOS system Complex I), ATP5A1 (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... UQCRC2 (Abcam, ab14745; OXPHOS system Complex III), NDUFA9 (Abcam ab14713 ...
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bioRxiv - Molecular Biology 2019Quote: ... COX5A (Abcam ab110262; OXPHOS system Complex IV), UQCRC2 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... synapsin-1 (Abcam #ab254349, Synaptic systems #106104), c-myc (Sigma #M4439 ...
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bioRxiv - Immunology 2022Quote: ... TSLP and pCol1 in cell culture supernatants were determined using ELISA kits (R&D System for Areg, IL-33 and TSLP, and Abcam for pCol1). Unless stated otherwise ...
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bioRxiv - Molecular Biology 2019Quote: ... MT-CO2 (Abcam, ab110258; OXPHOS system Complex IV), MRPL49 (Proteintech ...
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bioRxiv - Molecular Biology 2019Quote: ... MT-CO1 (Abcam, ab14705; OXPHOS system Complex IV), SDHA (459200 ...
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bioRxiv - Cell Biology 2021Quote: ... IFN-γ (RnD Systems) and BNP ELISA (Abcam) was used as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... BATF3 (R & D Systems, #AF7437) or TAp73 (Abcam, # ab14430). The immunocomplexes were then conjugated with ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology ...