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Citations for abcam :
301 - 350 of 1339 citations for Borrelia Miyamotoi GlpQ Protein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Protein quantification was performed with the BCA protein assay kit (ab207003, Abcam). Proteins were separated by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Oxysterol-binding protein-related protein 8 (anti-OSBP8/ORP8; Abcam, ab99069), anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A ...
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bioRxiv - Microbiology 2024Quote: ... Proteins on the gels were visualized using InstantBlue Coomassie Protein Stain (Abcam), destaining with distilled water.
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed twice in PBST before being incubated with the primary rabbit anti-E-tag antibody diluted 1/5,000 in PBST (Abcam, ab3397) for 1h at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were incubated with goat polyclonal antibodies directed against MOMP and rabbit polyclonal antibodies directed against 6xHis tag (Abcam) for 1 hour ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysis contained GFP tag strain was subjected to affinity purification by using Anti–GFP antibody (ab290, Abcam, Cambridgeshire, UK). The process of antibody purification was using GenScript Protein A MagBeads according to the manufacturer’s instructions (Catalog No ...
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bioRxiv - Biochemistry 2021Quote: ... The bead slurries used were c-myc-conjugated (EZview red rabbit anti-c-myc affinity gel, Sigma, or Agarose goat anti-myc tag antibody, Abcam) and ubiquitin binding domain (UBD)-conjugated matrix (Ubiqapture-Q ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria harboring an HA tag were washed with PBS and stained with anti-HA antibody conjugated with FITC (Abcam ab1208) at 10 µg/mL for 75 minutes in the dark on ice ...
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bioRxiv - Cell Biology 2022Quote: ... stable expression of target receptors was determined by staining against their N-terminal Flag-tag with mouse anti-Flag conjugated to Alexa 488 (Abcam).
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were incubated with the blots overnight at 4 °C in blocking solution: anti-myc-tag (1:5,000, rabbit pAb, ab9106, Abcam, UK), anti-Dclk1 (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Induced EBY100 was concurrently labeled with DyLight-488-MMP-14 (see below) and 1:100 PE Anti-Myc tag antibody (abcam) for 1 h 30’ at room temperature in TNCA (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... homozygous clones were further analysed by western blotting for homozygous knock-in of the tag using anti-UAP56 (ab181059; Abcam) and anti-GFP antibodies (A11122 ...
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NK cells orchestrate splenic cDC1 migration to potentiate antiviral protective CD8+ T cell responsesbioRxiv - Immunology 2020Quote: ... 1% Protein Block (Abcam), and 2% 2.4G2 supernatant ...
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bioRxiv - Physiology 2020Quote: ... and protein phosphatase (Abcam) inhibitor cocktails ...
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bioRxiv - Physiology 2022Quote: ... CFTR protein (ab2478, Abcam) and Na,K-ATPase α-subunit (ab76020 ...
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bioRxiv - Neuroscience 2023Quote: ... or Protein G (Abcam) beads were added and incubated overnight to preclear the supernatant ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membranes were blocked with 5% milk (w/v) in TBST (TBS, 0.1% Tween 20) and were probed with horseradish peroxidase (HRP) conjugated anti-His antibody (Abcam, catalog # ab1187) or the C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 20μL of 1X phosphate buffered saline (PBS) and anti-6x His Alexa Fluor 647 (abcam, cat: ab237337) was added at a dilution of 1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were kept in PBS until staining as described above in Immunohistochemistry using Rb pAb to HA tag (1:1000, Abcam ab9110). For image quantification in FIJI after Airyscan processing ...
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bioRxiv - Microbiology 2020Quote: ... Excess of BSA was removed and the wells are covered with the primary rabbit 1/500e anti-E-tag antibody (Abcam, ab3397) in 0.5% PBS 1X-BSA for 45 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... Mouse monoclonal anti-6xHis tag antibody and rat tubulin antibody which recognizes the C-terminal tail of α tubulin were purchased by ABCAM. GMPCPP was purchased from Jena Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed twice with PBST for 10 minutes and then incubated with HA-tag primary anti-rabbit antibody (Abcam ab236623) for 2 hours with shaking ...
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bioRxiv - Immunology 2020Quote: ... Antigen-coupled beads were validated by incubating them with serial dilutions of anti-histidine-Biotin antibody for antigens with a histidine-tag (Abcam, ab27025). To choose an appropriate coupling concentration ...
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bioRxiv - Cell Biology 2023Quote: Immunostaining was performed on CENP-T-based kinetochore-like particles bound to taxol-stabilized microtubules or oligomers immobilized on the coverslips functionalized by 10-min incubation with 20 µg ml-1 anti-S-tag antibodies (Abcam, ab87840) and blocked with 1% Pluronic F-127 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred onto a PVDF membrane and the membrane was blocked and then probed overnight with anti-HA tag antibody (1:2500, Abcam, ab9110). After primary Ab incubation ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein A sepharose beads (ab193256) and protein G sepharose beads (ab193259) were from abcam.
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bioRxiv - Molecular Biology 2020Quote: ... Plasma membrane proteins were extracted using the Plasma Membrane Protein Extraction Kit from Abcam. Proteins were separated from membrane and cytoplasmic extracts on 8% SDS-PAGE gels and then western blotted with antibodies against PKP2 (EB10841 ...
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bioRxiv - Immunology 2021Quote: ... S1 Spike protein was detected using a Covid-19 S-protein ELISA kit (Abcam) specific to S1RBD ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma membrane proteins were extracted using a plasma membrane protein extraction kit (Abcam, #ab65400), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Plasma membrane proteins were extracted using a plasma membrane protein extraction kit (Abcam, #ab65400), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant human PD-L1 protein and recombinant human tau protein were purchased from Abcam. HRP-conjugated goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonyl assays used the Oxidized Protein Western Blot Detection Kit (ab178020; Abcam, USA) and were performed as we previously described.36 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and NICD: Mdm2 (MDM2 protein Gln119-Leu438 Origene TP761916; MDM2 protein-full length Abcam, ab82080 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins on the gel were visualized by staining with InstantBlue Coomassie protein stain (Abcam).
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentrations were determined using the BCA Protein Quantification Kit (Abcam, Cambridge, UK, ab102536) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Both the supernatant and pellet (after washing with buffer F) were resuspended with SDS-loading buffer for SDS-PAGE and western blotting probed by mouse anti-His antibody (Abcam, MA, USA).
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773, Goat pAb to rabbit IgG)) at a 1:5,000 dilution to detect RhaPS and His-tagged carrier proteins ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant mouse WNT3a protein (Abcam) 50ng/ml was added and cells cultured for 8 hours before lysis in Trizol.
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bioRxiv - Bioengineering 2020Quote: ... Collagen IV protein (Abcam, ab7536) and Matrigel (growth-factor reduced ...
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bioRxiv - Physiology 2019Quote: ... total OXPHOS protein (Abcam, #ab110413), mt-Co2 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated Protein-G (abcam), and Alexa Fluor 488/555-conjugated anti-goat/mouse IgG donkey polyclonal antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant MAP2c protein (Abcam, #ab114686), carrying a ∼26 kDa Glutathione-S-Transferase (GST ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein blocking solution (Abcam). They were then incubated overnight at 4°C with primary antibody (Mouse TIF-IA (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... fibroblast activation protein (FAP, Abcam rabbit monoclonal antibody EPR20021 ...
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bioRxiv - Molecular Biology 2023Quote: ... green fluorescent protein (ab290, Abcam) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... followed by protein block (Abcam) for 30 minutes and were then incubated with relevant primary antibodies diluted in antibody diluent (Abcam ...