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Citations for abcam :
51 - 100 of 1935 citations for 5 FLUORO 2 MERCAPTOBENZOTHIAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% donkey serum for 2 h with primary antibodies against cardiac troponin I (ab47003, Abcam: 1:800) and for 1 h with secondary antibodies conjugated with Cy5 (711-175-152 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitation was performed overnight at 4°C with rotation using 1-2 × 106 cell equivalents per immunoprecipitation using antibodies (5 µg) against H3K4me1 (Abcam), H3K27ac (Abcam) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Neuroscience 2019Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 µM Pitstop-2 (Abcam), hypotonic medium (80% of medium was replaced with water ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were pre-incubated at room temperature for 2 hours in a blocking medium composed of PB-Tx and Normal Goat Serum (NGS, 5% or 10%, Abcam, Cat. No. ab7481), after which they were incubated with primary antibodies (72 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... α5 (Abcam, ab175195), β2 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... 5-LOX (Abcam), Pax2 (Abcam) ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2; ab196883) and monoclonal antibodies against phospho-ERK1/2 (p-ERK1/2; ab201015) were purchased from Abcam. Protein expression was normalized to GAPDH (sc-2577 ...
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bioRxiv - Immunology 2021Quote: ... 2-N-nitrobenz-2-oxa-1,3diazol-4-amino]-2 deoxy-D-glucose (2NBDG) (Abcam). CD4+ cells were cultured for 24h in anti-CD3 coated plates (0.5μg/ml ...
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bioRxiv - Genomics 2022Quote: ... A cocktail of antibodies against the glucocorticoid receptor was added to chromatin input for immunoprecipitation: 2 μg of ab3570 (Abcam, lot GR3222141-5, discontinued) and 0.49 μg of 12041S (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-5 Lipoxygenase (5-LOX) (rabbit, 78 kDa, ab169755, 1:1000, Abcam) and β-actin (mouse ...
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bioRxiv - Genomics 2023Quote: ... were blocked and pH-calibrated as per the manufacturer’s protocol and 5 μl bed volume of beads were loaded with 2 μg of ChIP antibody (H3K27ac; Abcam-AB4729, H3K115ac; PTM BIO-PTM-170). Beads were added to the solubilized chromatin ...
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bioRxiv - Neuroscience 2021Quote: ... 5 QX-314 (Abcam), and 5 sodium phosphocreatine ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Claudin-5 (Abcam), anti-Glut1 (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μM GsMTx4 (Abcam), 200 μM Jedi1 (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... CENPF (Abcam, ab-5) 1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tau 5 (Abcam, ab80579), Tubulin (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... bumetanide (5 µM, Abcam). R,S-MCPG and S-MCPG were prepared in equimolar concentrations of NaOH ...
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bioRxiv - Microbiology 2023Quote: ... 5 μM GSK872 (Abcam), 10 μM MCC950 (Selleck) ...
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bioRxiv - Molecular Biology 2022Quote: ... Aquaporin 5 (ab92320, abcam), Axin2 (ab109307 ...
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bioRxiv - Cancer Biology 2023Quote: ... FIDAS-5 (Abcam, UK), Azacytidine (AZA ...