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Citations for abcam :
4851 - 4900 of 4993 citations for Recombinant Mouse Fgfr1 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The grids were then washed with PBS+ three times for 2 min and incubated on a 20 ul drop of secondary antibodies conjugated with gold particles (1:20 dilution of anti-mouse and anti-rabbit secondary antibodies conjugated with 10nm and 6nm gold particles respectively, Abcam, Cambridge, UK). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... and subsequently incubated for 1 h with a 1:5000 dilution of a horseradish peroxidase-conjugated goat anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK) or goat anti-rabbit secondary antibody (ab6721 ...
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bioRxiv - Immunology 2023Quote: ... as described previously.25 IgA levels in lung homogenates were measured by ELISA with goat anti-mouse IgA a chain antibody conjugated to horseradish peroxidase (ab97235; Abcam, Cambridge, UK), as previously reported.10 MPO activity was measured in lung homogenates as reported previously25 ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... Both the supernatant and pellet (after washing with buffer F) were resuspended with SDS-loading buffer for SDS-PAGE and western blotting probed by mouse anti-His antibody (Abcam, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... The macrophages in the atherosclerosis in the thoracoabdominal aorta of the rabbits were revealed using mouse anti-rabbit CD68 antibody (Abcam, Cambridge, UK) as the primary antibody and goat anti-mouse IgG antibody (horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 h with 700 µL of a 1/250 dilution of anti-mouse IgG Alexa FluorTM 555 secondary antibody (ab150106; Abcam, Cambridge, UK) in MTSB + 5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed three times with PBST and then incubated with anti-mouse IgG or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cambridge, UK) in PBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The macrophages in the plaque in the thoracoabdominal aorta of the rabbits were revealed using mouse anti-rabbit CD16/32 and CD206 antibody (Abcam, Cambridge, UK) as the primary antibody and goat anti-mouse IgG antibody (HRP labeled ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice with PBST before being incubated with the primary antibody (Mouse anti-BrdU, 1:1000, Cat. No. B35128, Invitrogen; or Rat anti-BrdU, 1:1000, Cat. No. ab6326, Abcam, RRID: AB_305426) and the secondary antibody (Donkey anti-mouse 488 or 568 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used: anti-SOX2 diluted 1:500 (R&D AB2018, mouse) and anti-NR2F1 diluted 1:1000 (Abcam Ab181137, rabbit). Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-staining was conducted using mouse anti-β-tubulin 1E1-E8-H4 antibody (dilution 1:300, cat. nr. ab131205, Abcam, Cambridge, UK), or goat anti-collagen Iα1 antibody (dilution 1:20 ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with mouse Anti-GABAAR γ2 primary antibody (1:500; catalog no. ab307231, Abcam, United Kingdom) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (AF488 goat anti-mouse IgG, Abcam catalog #ab150113; AF594 goat anti-rabbit, Abcam catalog #ab150080; AF680 goat anti-rat IgG, Abcam catalog #ab175778) were diluted 1:500 in DPBS with BSA and Triton-X100 and incubated for 75 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... 0.4% Triton X) before being stained overnight at 4C with the following primary antibodies: mouse anti-APC (CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&DSystems #AF-420-SP ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C in primary mouse IgG2b anti-NeuN (1:500, Abcam cat# ab104224). Sections were washed twice the following day with 1X PBS followed by incubation in goat anti-IgG2b AlexaFlour 647 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the coverslips were incubated overnight (4 °C) with a mouse monoclonal anti-15-LOX-1 antibody (1:100, # ab119774, Lot: GR3322776-8; Abcam, Cambridge, UK), washed repeatedly ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... washed three times with PBS for and subsequently stained with AF647 anti-mouse secondary antibody (1:200, Abcam, ab169348 in 1% BSA) for 1 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264, St. Louis, USA; polyclonal rabbit anti-talin 1, 1:100, Abcam ab71333, Cambridge, UK) diluted in blocking solution for 1.5 h at room temperature or overnight at 4 °C.
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360. GFP; host: chicken; diluted 1:2000; Abcam; cat# ab13970) diluted in antibody solution (ABS ...
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bioRxiv - Molecular Biology 2020Quote: ... Bottom halves of dermal sample membranes were incubated with rabbit anti-TIMP3 (Cell Signalling Technology; D74B10) and top halves with mouse anti-HSA (Abcam, ab10241; loading control). Bottom half of epidermal sample membranes were incubated with rabbit anti-RPL36 (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed 3 times and then stained simultaneously with two secondary antibodies: goat anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:400 diluition; ab150117; Abcam, Cambridge, UK) and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... with 2-8 μg total protein loaded per lane and the following dilutions of mouse mAbs: anti-K14 (1:500; clone LL002, Abcam Inc., Cambridge, UK), anti-K42 (1:500) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the samples were incubated with Alexa Fluor 488- or 594-conjugated goat-anti-mouse or goat-anti-rabbit secondary antibodies (Abcam, 1:500 dilution) overnight at 4 °C and rinsed several times in PBST before mounting in ProLong Diamond Antifade mountant (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Afterward the sections were incubated with 1:100 diluted mouse polyclonal anti-GFAP (Cusabio, Houston, TX, USA) and 1:500 diluted rabbit polyclonal anti-HuC/D (Abcam, Cambridge, United Kingdom) overnight at 4°C· followed by incubation with Cy3-labeled goat anti-mouse IgG (Beyotime ...
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bioRxiv - Cell Biology 2021Quote: ... and for >16 hrs at 4°C with the following primary antibodies: mouse-α-CD63 (1:1000, clone TS63; Abcam, Cambridge, United Kingdom), mouse-α-Flotillin-1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... so membranes were also incubated with a mouse monoclonal anti-beta actin primary antibody (dilution: 1:5000, product number: ab8226, Abcam, Cambridge, MA, USA). After TBST washing three times for 10 minutes each wash ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-CaVβ3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). Membranes were then incubated in LiCor Blocking Buffer as described above and LiCor IRDye secondary antibodies ...
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bioRxiv - Physiology 2022Quote: Both neonatal and adult ducts were stained using antibodies against the cholangiocyte marker keratin 19 (K19, 1:10, Developmental Studies Hybridoma Bank, TROMAIII) and for the myofibroblast marker α-smooth muscle actin (mouse α-SMA, Abcam ab7817). Adult bile ducts were also stained for hematoxylin and eosin and Masson trichrome.
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore: MABE415, RRID: AB_2721039; 1:2000; Invitrogen: MA5-31419, RRID: AB_2787055; 1:2000; rabbit, Abcam: ab16046, RRID: AB_2107107; 1:500), Calb1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
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bioRxiv - Cell Biology 2022Quote: ... We used mouse anti-beta-tubulin (E7, Developmental Studies Hybridoma Bank, 1:300 dilution) and rabbit anti-histone H3 (Abcam, 1:500 dilution). Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... stained and incubated in a humidified chamber with rabbit anti-mouse Collagen I overnight at 4°C (1:100 dilution Abcam Catalog No. ab34710); washed 3 times in TBS-Tween (.05% Tween ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and incubated with the appropriate fluorescein-conjugated secondary antibodies (Alexa Fluor 594-labeled goat anti-mouse IgG from Abcam for γ-H2AX; Alexa Fluor 488-labeled goat anti-rabbit IgG from Abcam for RAD51). After washing ...
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bioRxiv - Immunology 2019Quote: ... The tissue was embedded in paraffin and sections were stained with an antibody against RELM-α (polyclonal rabbit anti-mouse, Abcam, Cambridge, UK). Nuclei were stained with haematoxylin (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
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bioRxiv - Developmental Biology 2020Quote: ... cover slips were incubated in primary antibody at 4°C overnight followed by rinses in PBS and one hour incubation with goat anti-mouse IgG (Alexa Fluor 647-conjugated) secondary antibody (Abcam, Cambridge, MA, ab150115) and/or goat anti-rabbit IgG (Alexa Fluor 594-conjugated ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-HA antibody (Covance, Burlington, NC: H11) and rabbit anti-GFP antibody (Abcam, Cambridge, UK: ab290) were used at 1:500 ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 9 was performed using citrate buffer and sections were incubated with the primary antibody rabbit anti‐mouse CD31 (1:50) (ab28364, Abcam, Cambridge, MA, USA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...