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Citations for abcam :
4701 - 4750 of 5058 citations for Recombinant Mouse Ifnar1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the indicated primary antibodies diluted in blocking buffer at 4°C overnight: mouse-pH3 (1:2000, Abcam, ab14955), rabbit-Ki67 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of mouse anti-c-fos primary antibody (1:1000 dilution, Abcam, UK) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... santacruz sc293), CDK7 (1/2000, rabbit, santacruz sc856X), CyclinH (1/1000, mouse, IGBMC 2D4) and p62 (1/500, rabbit, abcam ab204168). The loading was controlled with anti-α-tubulin antibody (1/50000 ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-glucagon (ABCAM; ab82270) primary antibodies ...
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bioRxiv - Microbiology 2019Quote: ... After blocking, the cells were stained with Mouse Anti-Human Myeloperoxidase (Santacruz, Germany) and Rabbit Anti-Human Histone-H3 (Abcam, USA) antibodies for one hour ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc., MA, USA; anti-MITF, mouse anti-MiTF, ab80651, Abcam plc. ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Laminin (1:500, DSHB 3H11), mouse anti-Msx1/2 (1:100, DSHB 4G1) and rabbit anti-RFP (1:500, Abcam ab62341).
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bioRxiv - Bioengineering 2021Quote: ... diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500 ...
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bioRxiv - Immunology 2019Quote: ELISAs were performed using Mouse MIP-1 alpha (CCL3) ELISA Ready-SET-Go ELISA kit (eBi-oscience™) and Prostaglandin E2 ELISA Kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The following antibodies were used: mouse anti-CENP-A (clone A1, 1:500,36) and rabbit anti-H3K9me3 (abcam 8898; 1:200). Microscope images were acquired on a DeltaVision Core system (Applied Precision ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were incubated for 2.5h with primary Abs for (IdU: 1:100, mouse monoclonal anti-BrdU, Becton Dickinson 347580; CldU: 1:100, rat monoclonal anti-BrdU, Abcam 6326) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were blocked with 1% normal goat serum (30 min at room temperature) followed by a goat anti-mouse IgG2a antibody (Abcam, ab98694). After additional blocking with normal goat serum ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The following primary antibodies and their respective dilutions were used in immunofluorescence (IF) and Western blot (WB) experiments: MITF (C5) mouse monoclonal (Abcam, #ab12039), 1:2000 (WB) ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... western blotting (WB) and WB blocking conditions were as follows: mouse anti-β-actin (Abcam, 8226, WB 1:2000 in BSA), mouse anti-ZEB2 (Origene ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1hr and probed overnight with primary antibodies (Rabbit anti-SDMA, CST13222; mouse anti-vinculin, Sigma, RABBIT anti-PRMT5 (Abcam Ab31751). Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... Human and mouse lung sections were stained for colocalization with primary and secondary antibodies: ANT1 (Abcam #ab102032 rabbit polyclonal, 1:1000), pan ANT (anti-ANT1/2/3 Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... sections with primary antibodies were incubated with secondary antibodies (Alexa Fluor-594 goat anti-rabbit IgG, #a21429, Thermo Scientific; Alexa Fluor 488 goat anti-mouse IgG, #ab-150116, abcam; Alexa Fluor 594 goat anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were then washed 5x with TBST and probed with 1/5000 FITC-labelled goat anti-mouse IgG secondary pAb (Abcam ab6785) and imaged for fluorescence on the Typhoon FLA7000 biomolecular imager (GE healthcare).
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bioRxiv - Cell Biology 2021Quote: ... with secondary antibody goat anti-mouse IgM mu chain (dilution 1:2000) conjugated with Alexa Fluor 488 (ab150121, Abcam, Cambridge, UK). Only one primary antibody was used in each experiment.
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bioRxiv - Microbiology 2020Quote: ... Anti-mouse IgG conjugated to HRP for immunoblot of immunoprecipitation samples (used in Fig. 3b) was purchased from Abcam (Cat#: ab131368). Fluorophore-conjugated secondaries for immunofluorescence were purchased from Life Technologies.
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bioRxiv - Bioengineering 2021Quote: ... Primary antibody solutions were prepared by diluting one or more of the following antibodies in blocking buffer: mouse anti-HNF4a (1/200 from stock, Abcam ab41898), goat anti-OPN (1/60 from stock ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by overnight incubation at 4 °C in 1% BSA with primary antibodies: mouse monoclonal [EA-53] to sarcomeric alpha-actinin (Abcam ab9465), rabbit polyclonal anti-phospho-Histone H3 (Ser10 ...
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bioRxiv - Microbiology 2021Quote: ... Hemoglobin and hemopexin levels in the plasma samples of WT- and KO-infected mice were measured by Mouse Hemoglobin (Abcam, ab157715) and Mouse Hemopexin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were then added: mouse anti-BrdU/nuclease (1:100) (Amersham) and rabbit anti-αTubulin/1% BSA/1x PBS (1:500) (Abcam Ab52866) and incubated for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Microbiology 2020Quote: ... 3 colonic and 1 intestinal tissue fragments of each mouse were incubated with a primary polyclonal 1:200 rat anti-E-cadherin (#ab11512; Abcam USA) in PBS–3% BSA for overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... Embryos were washed (3x 30 min PBS-T) and then secondary antibody (goat anti-mouse Alexa Fluor® 555 - abcam ab150114) at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605 ...
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bioRxiv - Immunology 2022Quote: ... Lysates were separated by SDS-PAGE and western blotted with rabbit anti-ERAAP (1:1000) or mouse anti-GAPDH (1:5000; Abcam, ab8245). Rabbit anti-ERAAP was produced in the Shastri lab.
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bioRxiv - Developmental Biology 2022Quote: ... The cells were washed with 0.3% PBSTx four times for 15 min per wash and incubated in Alexa Fluor 488 Goat Anti-Mouse IgG H&L (Abcam, ab150117) at a 1:1000 dilution at room temperature (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse monoclonal anti-human-ATM(phospho-S1981) which stains the homologous mouse ph-ATM(phosphor-S1987) was used at 1:500 (Abcam ab36810). The slides were incubated in biotin-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Biochemistry 2022Quote: ... Protein tech 50430-2-AP), anti-RFP (rabbit, 1:3 000, St. Johns Laboratory STJ97083) and anti-H2B (mouse, 1:3 000, Abcam, ab52484). Membranes were washed 3 times for 10 min each in PBST ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were then applied (IB4-Alexa 647, 1:300, ThermoFisher I32450; goat anti-mouse Alexa-488, 1:500, Abcam ab150113; FITC goat anti-GFP, Abcam ab6662) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: In a separate plate the MYH Alpha-LISA was performed using 1:2,000 dilution of mouse monoclonal BA-G5 antibody to α-MYH (abcam, ab50967), 1:2,000 dilution of rabbit polyclonal β-MYH (abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... β1-integrin blocking antibodies at 100 μg/mL (MilliporeSigma; # MABT821) or mouse monoclonal (P5D2) β1-integrin blocking antibodies at 50 μg/mL (Abcam; ab24693).
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bioRxiv - Physiology 2022Quote: ... and cells were washed in 3 changes of PBS prior to incubation with secondary antibody (DyLight 488 anti-rabbit IgG (ab96881) or DyLight 594 anti-mouse IgG (ab96881) (Abcam, UK)) for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies raised against α-syn C-terminus (Mouse anti α-syn, Amino Acid (AA) 124-134 (1:1,000) and ab1903 (Abcam); Mouse anti α-syn 4B12 ...
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bioRxiv - Plant Biology 2022Quote: ... After three washes with 1 x PBS the samples were incubated for 4 hours with anti-mouse secondary antibodies conjugated with Alexa Fluor 488 (ab150077, Abcam, UK) diluted 1:400 in PBS ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with secondary antibodies Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (1:700; Abcam, ab150115) and Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488 ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibody used were GFAP (GA5) Mouse mAb (1:600; Cell Signalling Tech, 3670S) and rabbit anti-C1q antibody (1:250; Abcam, ab182451). The secondary antibody used were Goat Anti-Mouse IgG H&L (Alexa Fluor® 647 ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... cells were blocked with 10% Normal Goat Serum for 1 h and incubated in the appropriate primary antibodies from mouse or rabbit: Sox2 (1:1000, Abcam, ab92494), Oct4 (1:250 ...