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Citations for abcam :
4601 - 4647 of 4647 citations for FGF acidic Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (Alexa Fluor 488) antibody (ab150081) and goat anti-mouse IgG (Alexa Fluor 594) antibody (ab150120) were from Abcam (Cambridge, MA, USA). SAG (Smoothened Agonist ...
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bioRxiv - Developmental Biology 2021Quote: ... secondary antibodies conjugated with fluorescence dyes (1:5000; anti-mouse IRDye® 800CW and anti-rabbit IRDye® 680RD; Abcam, ab216774 and ab216779, respectively) were treated at room temperature for 3 h ...
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bioRxiv - Bioengineering 2019Quote: ... cells were resuspended at a concentration of 2×106 cells/ml in serum-free media containing 30 μg/ml of an IgG mouse isotype control antibody (abcam, Cambridge, MA, Catalog #ab91353) or a function blocking anti-β1 integrin antibody (abcam ...
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bioRxiv - Neuroscience 2021Quote: Fixed CNS tissue was cryosectioned (20 μm) and stained overnight with primary antibodies against: ERα (EMD Millipore, #C1355 polyclonal rabbit or Abcam, #93021 monoclonal mouse), phospho-Serine 244/247 RPS6 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were incubated in primary antibody (mouse anti-TH antibody, 1:2000, cat#: MAB318, Sigma-Aldrich, St. Louis, MO, USA; or mouse anti-NET antibody, 1:1000, cat# 211463, Abcam, Cambridge, MA, USA) for 48 h at 4 °C or overnight at room temperature (RT) ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Immunology 2021Quote: ... detected with HRP conjugated secondaries in blocking buffer (Goat anti-Rabbit HRP, Sino Biological SSA003, 0.5 μg/ml or Goat anti-Mouse HRP, Abcam ab205719, 1: 4000 dilution) and visualised using the SuperSignal West Femto kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed 3x with TBS-T before incubating in secondary antibodies goat anti-mouse IgG H&L (GAPDH) and goat anti-rabbit IgG H&L (ROR2) (Abcam, both IRDye® 800CW) in TBS-T for 1hr at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 0,3% Triton in PBS and incubated overnight with primary antibody (Guinea pig anti-Iba1, 1:500 [abcam] and mouse anti-NeuN, 1:500 [abcam]). Slides were washed 3 × 30 min and incubated with secondary antibodies (Donkey anti-mouse 405 ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933; 1:1000 monoclonal mouse anti-β-Actin, Abcam, AB8226) followed by secondary antibodies (1:4000 donkey anti-goat ...
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bioRxiv - Cancer Biology 2019Quote: ... and secondary 1:1000 anti-mouse Alexa Fluor® 488 IgG H&L ab150113 and anti-rabbit DyLight® 594 ab96885 (both Abcam, Cambridge, UK). All tumouroids were counterstained with DAPI ...
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bioRxiv - Bioengineering 2020Quote: ... cells were incubated at room temperature for 1 hour with the secondary antibody Goat Anti-Mouse IgG H&L-Alexa Fluor® 488 (Abcam, Cambridge, MA, ab150113) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... The CldU and IdU tracks were detected by IF with anti BrdU antibodies (rat for CldU, Abcam ab6326, and mouse for IdU, BD 347580). Fiber integrity was assessed with a mouse anti-ssDNA antibody (Millipore MAB3034) ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-Rabbit IgG DylightTM 549 antibody (KPL, 042-04-15-06, 1:500) or anti-Mouse IgG Dylight® 550 antibody (Abcam, ab96876, 1:500) or anti-Goat IgG DylightTM 549 antibody (Rockland ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:1000 dilution) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The higher molecular weight part of the membrane was incubated overnight at 4ºC in anti-DNAK antibody (1:5000 dilution in blocking buffer, Abcam#69617 Mouse monoclonal [8E2/2]) as the loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies Goat Anti-Mouse IgG H&L (HRP) (ab205719) and Goat Anti-Rabbit IgG H&L (HRP) (ab205718) were purchased from Abcam (Abcam, Cambridge, United Kingdom). All antibodies were used in dilutions according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... washed four times with PBS-T and 20 μL of polyclonal HRP-conjugated detection antibodies (goat anti-mouse, #G-21040, Thermo Fisher at 1:1,000 dilution or goat anti-human, #ab7153, Abcam at 1:10,000 dilution) were added to each well and incubated for 30 min (anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... then with horse radish peroxidase-conjugated goat anti-mouse (ASYN) and goat anti-rabbit (β-actin) secondary antibodies (1:5000; Abcam, RRID:AB_955413 and RRID:AB_955417). The signal was visualized with a chemiluminescent substrate and detected with BioRad ChemiDoc ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit, 1:200), GLP-1R (Mab 7F38-s, Developmental Studies Hybridoma Bank, host: mouse, 1:10) and GFP (AB16901, Abcam, host: chicken, 1:500). Secondary antibodies for confocal anti-mouse 568 nm (A11004 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 1% normal goat serum (NGS) blocking solution as follows: anti-rhodopsin 1D4 for mouse rod opsin (1:200; Abcam, St. Louis, MO, USA), R/G cone opsins (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with pre-adsorbed secondary antibodies (1:500 Goat anti-mouse IgG AF647, 1:500 Goat anti-rabbit IgG AF555; Abcam cat. no. ab150119 and ab150086) for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with pre-adsorbed secondary antibodies (1:500 goat anti-mouse IgG Alexa Fluor 488, 1:500 goat anti-rabbit IgG Alexa Fluor 555; Abcam cat. no. ab150119 and ab150086) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... before incubation with primary antibodies in blocking buffer (Rabbit polyclonal anti-SARS-Cov2, Sino Biological 40591-T62, 1:6000 dilution or Mouse b-actin, Abcam 8226, 1 μg/ml), detected with HRP conjugated secondaries in blocking buffer (Goat anti-Rabbit HRP ...
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bioRxiv - Biophysics 2019Quote: ... Primary antibodies used were mouse anti-Dlg (4F3 Hybridoma bank, 1:500; eye discs) and rabbit anti-aPKC (Abcam, AB5813, 1:500; pupal wing discs).
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bioRxiv - Synthetic Biology 2022Quote: ... then incubated for 1 h at 37 °C in the presence of a horseradish peroxidase– conjugated goat anti-mouse IgG antibody (Abcam, Cat # ab97265; diluted 1:25,000). After three PBS-T + 0.3% BSA washes ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated with the appropriate secondary antibody (anti-rabbit 488 Alexa Fluor, ab150077, Abcam or anti-mouse 488 Alexa Fluor, ab150113, Abcam, 1:1,000 in preblock buffer) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:500 rabbit anti-PARP1 Abcam ab227244; 1:500 rabbit anti-PCNA Abcam ab18197, 1:500 mouse anti-PCNA Abcam ab29 diluted in blocking solution). After antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 8M urea solution for 20 min (Malka et al., 2007) and decorated for 1h30min with mouse monoclonal anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (abcam, ab14734, dilution of 1/400) as a mitochondrial marker ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Biochemistry 2024Quote: ... The coverslips were washed twice with 1x PBS and incubated with the secondary antibody (donkey anti-mouse coupled to Alexa Fluor 594, Abcam; 1:500 in blocking buffer) for 60 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated in secondary antibodies (Alexa594-ocnjugated donkey anti-mouse and Alexa488-conjugated anti-rabbit, both from Abcam and both diluted 1:250 in block) and washed again as above ...
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bioRxiv - Microbiology 2024Quote: ... in PBS (BSA/PBA) for 45 mins (RT) and then stained against external calreticulin using mouse monoclonal anti-calreticulin (Abcam, Cat. No. ab22683, 1:500), or mouse monoclonal primary anti-LAMP1 (Cell Signalling ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated with specific primary antibodies (mouse anti-Esrrb, Perseus PP-H6705-00; rabbit anti-Klf4, Santa Cruz, sc-20691; rabbit anti-Tfcp2l1, AbCam, ab123354; anti-βactin, Sigma, A3854). Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes with transferred proteins were blocked/permeabilized for 1 hour in 5% BSA in TBS and incubated with different antibodies (mouse anti-pMLKL, Millipore, rabbit anti-MLKL, Abcam, and rabbit anti-Hsp90, Santa Cruz) overnight at 4 °C ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were then incubated overnight in primary antiserum (rabbit anti-S100, 1:300, Abcam; mouse anti-NeuN, 1:300, Abcam; rabbit anti-WFS1, 1:300, proteintech) diluted in PBS with 3% NGS and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: chicken polyclonal anti-mouse GFAP (Abcam ref. ab4674, RRID AB_304558, 1:3000), rabbit polyclonal anti-mouse IBA1 (Wako ref ...
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bioRxiv - Immunology 2024Quote: ... overnight at 4°C and the relevant secondary for 1h at room temperature (Streptavidin-AF488 Invitrogen S11223 at 2 µg/mL final, anti-mouse IgG1-Cy5 Abcam ab136127 at 1.5 µg/mL final). After nuclear staining with DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated first with a polyclonal rabbit anti-SNED1 antibody (used at a 1 μg/mL concentration, previously described [40]) and a monoclonal mouse anti-GFP antibody (Abcam #ab1218, used at a 1:500 dilution), and then with a goat anti-rabbit antibody conjugated to AlexaFluor568 (Thermo Fisher A11036 ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections were then incubated in primary antiserum (rabbit anti-c-Fos, 1:300, Cell Signaling; rabbit anti-PV, 1:300, Abcam; mouse anti-HA-tag, 1:300, proteintech) diluted in PBS with 3% NGS and 0.1% Triton X-100 overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557, Lot# 066M4858V; rabbit α- α- tubulin, Abcam, Cat# AB233661) and in secondary antibody for two hours at room temperature (Alexa goat α-chick 488 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit. 488,1:500, ab150077, abcam; α-goat. 594, 1:500, ab150077, abcam; α-rabbit. 647, 1:500, ab150075, abcam). Nuclear and actin counterstains were performed using Abcam iFluor conjugated phalloidin (ab176753 ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019, used at 1:200 dilution]; and mouse anti-Sarcomeric α-actinin [Abcam ab# ab9465, used at 1:500 dilution]). Cell were washed three times with DPBS containing 0.2% Tween-20 ...