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Citations for abcam :
4501 - 4550 of 10000+ citations for Goat Anti Human IgG FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... was used to detect the presence of human CXCL10/IP-10 in the supernatants of infected or stimulated A549 cells using TMB (Abcam) as the substrate solution and 0.3 M H2SO4 as the stop solution.
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bioRxiv - Physiology 2021Quote: ... Schiaffino and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa Department of Biology as well as laminin (#11575; Abcam) and 10% goat serum ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion by HepG2 cells encapsulated in liver models with or without vasculature channels was quantified using ELISA (Human Albumin ELISA kit, Ab 108788, Abcam). Liver models (polymer composition ...
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bioRxiv - Cancer Biology 2021Quote: ... based on hematoxylin and eosin (H&E) staining and nuclear immune-positivity for human-specific NCL (ab13541, Abcam, Cambridge, UK) as previously described [21] ...
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bioRxiv - Bioengineering 2019Quote: Levels of brain natriuretic peptide (BNP) secreted in the media were quantified by Human BNP ELISA Kit (Abcam, cat# ab193694) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... day 1st-stimulated CAR T cells were co-cultured with γ-irradiated target cells at a 1:3 E:T ratio in the presence of 10 μg/ml plate-bound human CD226 blocking monoclonal antibody (clone DX11, Abcam, UK). Ultra-LEAF™ purified mouse IgG1(Clone MOPC-21 ...
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bioRxiv - Molecular Biology 2021Quote: Human RIPK3 rat monoclonal 1H2 (generated in-house)(Petrie et al, 2019b) Human phospho-MLKL (Ser358) rabbit monoclonal ab 187091 (Abcam) VDAC1 rabbit polyclonal AB10527 (Millipore)
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Immunology 2020Quote: Cytokine levels in the cellular supernatant were evaluated using the FirePlex-HT assay system with the Human Cytokines FirePlex-HT Panel 1 (ab234897; Abcam). FirePlex-HT immunoassays quantify up to 10 protein analytes per sample from low sample inputs in 384-well plate format ...
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bioRxiv - Cancer Biology 2020Quote: ... rehydrated through an alcohol gradient to water and antigen retrieval in citrate buffer pH 6.0 was used for the human nucleolin antibody at 5.0 g/mL (ab13541) (Abcam, Canbridge, MA). Endogenous peroxide activity and nonspecific binding was blocked with 3%(v/v ...
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bioRxiv - Cell Biology 2022Quote: ... The assays were carried out in 50 μl mTOR reaction buffer with or without 200 μM ATP and/or the recombinant human His-tagged ATF4 (Cat. ab109946, Abcam) at 30°C for 45 min ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies (Ab) used in this study: rabbit polyclonal Ab to human serum albumin (Abcam, catalogue no. ab2406, 1:400), goat polyclonal antibody to E-cadherin (R&D systems ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Microbiology 2024Quote: To treat cells with rPAI-1 we used recombinant active human PAI-1 (carrying stabilizing mutations N150K, K154T, Q319L and M354I, Abcam) in experiments on human airway epithelial cultures and in in-vitro complex formation assays ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The human origin of all patient-derived organoid xenografts (PDOXs) was confirmed by staining with an antibody recognizing human mitochondria (ab92824, Abcam).
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bioRxiv - Systems Biology 2023Quote: ... PVDF membranes were blocked and stained with total OXPHOS human western blot antibody cocktail (Abcam Cat# ab110411, RRID:AB_2756818, 1:500). Blots were visualized using an Odyssey infrared imaging system (RRID:SCR_022510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Labelling of c-Myc positive cells was performed with human c-Myc antibody (Abcam rabbit ab#32072 dilution 1:200) and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633) ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Neuroscience 2024Quote: ... ATP5a and VDAC1 were all validated in IHC in paraffin embedded tissues and tested in human samples according to the supplier (Abcam).
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bioRxiv - Neuroscience 2024Quote: ... Initial recombinant protein calibrators used in this study that can also be used as protein standards spiked into relevant detection matrices included LRRK2 [G2019S] recombinant human protein (ab177261, Abcam) and recombinant Rab10 (NBP2-23392 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cytokine concentrations were quantified using the human Cytokine Antibody Array following the manufacturer’s protocol (#ab133997, Abcam Inc., Toronto, ON, CAN). 1 mL of clarified media was applied per cytokine membrane ...
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bioRxiv - Neuroscience 2024Quote: ... Plasma GLP-1 concentrations were analyzed by an experimenter blinded to treatment conditions in the DRC Radioimmunoassay and Biomarkers Core at Penn using the Human GLP-1(7-36) ELISA Kit (ab184857; Abcam) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... rehydrated tissue was stained using an antibody for human mitochondria cytochrome c oxidase subunit II (MTCO2; 1:1000, rabbit polyclonal antibody, Abcam). Wash with PBS and stain fluorescence-tagged rabbit secondary antibodies (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Protein-nucleosome binding assays were performed by incubating the abovementioned purified Pioneer-seq nucleosome library with human TP53α (Abcam ab84768), TAp63α (Origene TP710041) ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-chicken, anti-rabbit, anti-mouse or anti-rat with fluorochromes (488, 568 or 647) at 1:500 (Alexa Fluor, abcam). They were incubated for one hour in the blocking solution containing Hoechst (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (rabbit anti-AQP4, 1:500, MerckMillipore; rabbit anti-GFAP, 1:500, Agilent Technologies; mouse anti-GLUT1, 1:250, Abcam) were added in PBS and incubated overnight at 4 °C on a rocking table ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared chromatin for both anti-H3K9me2 or anti-MYC ChIP samples was incubated with either 1μL of anti-H3K9me2 antibody (Abcam, ab1220) or 2μL anti-MYC antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... or rabbit anti-DJ-1 (1:1000, Novus), and chicken anti-Map2 (1:1000, Aves) or mouse anti-Map2 (1:1000, Abcam). Appropriate secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (1:100 mouse anti-ASCL1, BD 556604; 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326 ...
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bioRxiv - Neuroscience 2022Quote: ... with 0.2% Triton X-100 supplemented with 10% FBS and then incubated overnight at 4 °C with primary antibodies (1:100 mouse anti-ASCL1, BD 556604; 1:800 rat anti-BrdU, Abcam ab6326; 1:400 chicken anti-DCX, Abcam ab153668 ...
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bioRxiv - Neuroscience 2020Quote: ... These microglia cells were incubated with primary antibodies (mouse anti-Iba1 antibody, abcam, 1:400; rabbit anti-CD68 antibody, AbD Serotec, 1:100; rabbit anti-iNOS antibody, abcam,1:50 ...
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bioRxiv - Neuroscience 2020Quote: ... the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA, 1:2000, MilliporeSigma anti-parvalbumin, 1:2000, Abcam; anti-somatostatin ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody incubation was overnight at 4°C (anti-RIF1, Bethyl Laboratories A300-568A; anti-MCM4, Abcam ab4459; anti-GFP, Abcam ab290 ...
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bioRxiv - Genomics 2019Quote: ... Anti-HNF1A and anti-UTX stainings were performed at a dilution of 1:250 (Anti-HNF1A, Abcam ab204306, Cambridge, UK), 1:200 (Anti-HNF1A ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β, 1:2000, Ab41548, Abcam) diluted in the blocking solution ...
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bioRxiv - Plant Biology 2022Quote: ... non-fat milk and 0.05% Tween 20 and then incubated with primary antibodies (Anti-HYL1, 1:2000, Agrisera, AS06136; Anti-cFBPase, 1:5000, Agrisera, AS04043; Anti-Histone H3, 1:3000, abcam, ab1791 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were then incubated with the secondary antibodies (donkey anti-rat 405, Abcam, ab175670, donkey anti-rabbit 488, Abcam, ab150061, donkey anti-mouse 568, Abcam, ab175700 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-mTOR (1:1000, Cell Signalling), rabbit anti-CREB (1:1000, Cell Signalling) and rabbit anti-FKBP52 (1:1000, Abcam). Then the membrane was washed in TBS-T four times for 5 min and incubated for one hour in the dark at RT with secondary antibodies diluted in OBB/TBS-T(1:1) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were blocked in 2% BSA with 10% NGS in TBS followed by incubation in primary antibodies (guinea pig anti-CAPS2 (0.5 μg/ml) or rabbit anti-synaptoporin (1 μg/ml) or rabbit anti-GFP (Abcam, USA) in 2% BSA in TBS at 4 °C for 2 O/N and respective 1.4 nm gold-conjugated secondary antibodies (Nanoprobes Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then incubated with primary antibodies for 1 hour at 37 °C (1:250 mouse anti-MSH2 Abcam ab52266; 1:500 rabbit anti-PARP1 Abcam ab227244; 1:500 rabbit anti-PCNA Abcam ab18197 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917, 1:500 dilution; anti-GluA1, Merck-Millipore, AB1502, 1:1000 dilution; anti-phosphoSer845GluA1, Abcam, ab76321 ...
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bioRxiv - Genetics 2024Quote: ... Membrane was incubated for 30 minutes in 1% skim milk for blocking and afterward incubated with primary antibody for over-night (anti-PCNA: Sc65598-santa cruz. anti-Flag:F1804-Sigma Aldrich, anti-H3: ab1791-abcam. Anti-srs2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (rabbit anti-ac4C, #ab252215, Abcam; rabbit anti-NOP58, #14409-1-AP, Proteintech; mouse anti-NCL, #87792, CellSignal; mouse anti-G3BP, #ab56574, Abcam) were diluted 1:50 in blocking solution and incubated with the samples for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500, Abcam; rabbit anti-SATB2, 1:500, Abcam; rabbit anti-CTIP2, 1:500, Abcam; mouse anti-VGluT2, 1:500, Abcam; rabbit anti-cleaved caspase 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with primary antibodies (polyclonal rabbit anti-GFP, 1:500, Abcam; rabbit anti-SATB2, 1:500, Abcam; rabbit anti-CTIP2, 1:500, Abcam; mouse anti-VGluT2 ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with anti-Myosin 7A (Proteus Biosciences) or anti-Parvalbumin (Hudspeth Laboratory) primary antibodies and donkey anti-rabbit (Abcam) secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 3 hours at RT in secondary antibody (anti-rat AlexaFluor-488, #A21208, or anti-mouse AlexaFluor-488, Invitrogen #A11001, and anti-chicken AlexaFluor-647, Abcam #ab150175 ...