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Citations for abcam :
401 - 450 of 3134 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... immunoblotting using anti 6×His antibody was performed using protein G-HRP (Abcam, #ab7460; 1:5000 dilution). In pull-down assays using Ni-NTA ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 2 hrs with anti-αSYN ab52168 (Abcam, 1:100 dilution) in blocking buffer ...
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bioRxiv - Physiology 2021Quote: Differentiated Caco-2 cells in transwells were stained with H&E stain (Abcam, ab245880) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... live single-cell preparations were stained with 20μM 2’,7’ – dichlorofluorescin diacetate (DCFDA, Abcam) for 5 minutes in HBSS ...
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bioRxiv - Neuroscience 2023Quote: ... LRRK2 kinase activity was inhibited pre-treating cells with 0.5μM MLi-2 (ab254528, Abcam) for 90 minutes.
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bioRxiv - Immunology 2024Quote: ... cells were incubated with primary antibody for 2 h Anti-TFEB Antibody (Abcam, ab2636). After washing 3 times in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... p-LATS1/2 (T1079/T1041) (Cell Signaling, D57D3, Abcam, ab305029, 1:1,000 – 1:3,000), cJUN (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) cell proliferation assay (Abcam, ab197010). Gadodiamide (GdDTPA-BMA ...
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bioRxiv - Plant Biology 2020Quote: ... the fixed sections were probed with the anti-AcV5 tag primary mouse monoclonal antibody (Abcam, Cambridge, UK) and the Alexa Fluor 488 goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... Bound proteins were resolved by SDS-PAGE and detected using an αMYC-tag HRP-conjugated antibody (Abcam).
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bioRxiv - Neuroscience 2021Quote: ... Dynabeads were first washed in 1X PBS before 10µL Rabbit α-HA tag antibody (Abcam, #9110, RRID:AB_307019) was added to 300µL PBS+60µL Dynabeads and rotated at 4°C for 4hr ...
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bioRxiv - Molecular Biology 2022Quote: The following primary antibodies were used for CUT&Tag at 1:100 dilution: α-HSF1 (Abcam, ab2923), α-HSF2 (32) ...
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bioRxiv - Microbiology 2023Quote: ... and the S-tag lanes were reacted with HRP Goat anti Rabbit IgG (Abcam 6721, 1:25,000) for 1.5hr ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4° C overnight in 1:500 recombinant rabbit anti-HA tag (Abcam, AB236632) or rabbit anti-c-Fos (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphorylated GST-MIK1CD and His-MDISCD proteins are detected using an antibody against pSer/pThr (ab9332, Abcam). In addition ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were electroblotted onto a polyvinylidene difluoride membrane and probed with antisera against AcV5 tag (Abcam, Cambridge, UK), ZmPPDK (provide by Chris Chastain ...
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bioRxiv - Molecular Biology 2019Quote: ... and were subjected for incubation using Myc-tag antibody (ab32, UK, abcam, diluted in PBS containing 3%BSA) for 1h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The expression of the mEmerald tag was verified using a polyclonal anti-GFP antibody (Abcam, ab290 @ 1:5000). For hiPSC-CMs infected with SARS-CoV-2 (MOI 0.01 ...
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bioRxiv - Biochemistry 2022Quote: ... rabbit polyclonal antibodies against HA tag (1:1000, proteintech) and chicken polyclonal antibodies against MAP2 (1:10,000, Abcam) were used in the immunofluorescence imaging ...
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bioRxiv - Physiology 2020Quote: ... We used the following primary antibodies: rabbit-anti-S-tag (for S-tagged NTD, 1:100, Abcam, ab180958) and mouse anti-calmodulin (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the coverslip was activated by incubation with 20 µg/ml anti-S-tag antibodies (Abcam) diluted in BRB80 buffer for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were first stained with following antibodies: rabbit-anti-human pCD3ς (Abcam, EP776(2)Y) ...
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bioRxiv - Genetics 2020Quote: ... in HUDEP-2 cells expressing an HA-tagged ZNF410 construct or anti-ZNF410 (Abcam #ab174204) to detect endogenously expressed ZNF410 in HUDEP-2 and primary human CD34+ cells as well as endogenously expressed Zfp410 in MEL cells ...
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bioRxiv - Physiology 2021Quote: ... HEK-293 cells were cultured and treated with 2 µg/mL of tunicamycin (ab120296, Abcam) for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The transfected cells were stained with 1:100 dilution of anti-mouse BST-2 (Abcam) or isotype control antibody (R&D system ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell nuclei were stained with 4’,6-diamidino-2-phenylindole staining solution (ab104139, Abcam) at an ambient temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 20□μM of 2’,7’– dichlorofluorescin diacetate (DCFDA) (ab113851) (Abcam, USA) for 30□min at 37□°C and analysed on an Accuri C6 flow cytometer (BD Biosciences ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2; ab196883) and monoclonal antibodies against phospho-ERK1/2 (p-ERK1/2; ab201015) were purchased from Abcam. Protein expression was normalized to GAPDH (sc-2577 ...
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bioRxiv - Immunology 2021Quote: ... 2-N-nitrobenz-2-oxa-1,3diazol-4-amino]-2 deoxy-D-glucose (2NBDG) (Abcam). CD4+ cells were cultured for 24h in anti-CD3 coated plates (0.5μg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% Tween 20) and then probed with horseradish peroxidase (HRP) conjugated anti-His antibody (1: 5,000) (Abcam, catalog # ab1187) or rabbit polyclonal serum ...
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bioRxiv - Bioengineering 2020Quote: Tissue levels of MMP12 in Hi-Myc and healthy FVB/NJ prostate homogenates were measured by ELISA according to manufacturer’s protocol (ab246540, abcam). Prostate homogenates were prepared as previously described ...
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bioRxiv - Biochemistry 2021Quote: ... and the purified protein band was also confirmed by Western blot analysis by using an anti-His antibody (Abcam).
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bioRxiv - Microbiology 2020Quote: ... In a first step the membranes were incubated either with 0.1 μg/mL Anti-6×His® antibody (Abcam) to detect EF-P ...
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bioRxiv - Microbiology 2024Quote: ... 0.05 % Tween20) and then incubated with horseradish peroxidase (HRP) conjugated anti-His antibody (1:10,000 dilution; Abcam, United Kingdom) in TBST with 5 % (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... anti-V5 tag (Thermo Fisher Scientific, R960-25; IB 1:5000, IP 1μg, and Abcam, Ab53418; IF 1:800), anti-GFP (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: The immunodetection of G5845-1508 proteins were performed with different antibodies depending on the protein tag (anti-CBP (Abcam) for G5845-1508-TAP ...
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bioRxiv - Microbiology 2022Quote: ... protein expression was determined by Western blotting using as primary antibodies anti-FLAG or anti-V5 tag antibody (AbCam; 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... the samples were incubated at 4°C for 24 h with anti-V5 tag monoclonal antibody (ab27671, Abcam, UK) at a 1:20 dilution at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... ROR1 scFvs also contained a VSV-g tag that were detected by anti-VSV-g polyclonal antibody (FITC, Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... The identity of all truncated forms of eIF3g was confirmed by western blotting using antibodies against His6-tag (Abcam).
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bioRxiv - Immunology 2021Quote: ... Lymph node tissue sections were stained with human anti-spike antibody P2B-2F6 (72) (1:50) and biotinylated goat anti-human secondary antibody (Abcam, catalog no. ab7152, 1:300), followed by streptavidin-HRP reagent (Vectastain Elite ABC-HRP Kit ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... cells were subjected to ChIP assay with primary antibody against ESR1 (2 μl/IP, Abcam #ab32063) or normal rabbit IgG (1 μg/IP ...
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bioRxiv - Pathology 2022Quote: ... The pull-down reactions were analyzed by SDS-PAGE followed by Western Blot using anti-His (M20001; Abmart) and anti-GST (ab111947; Abcam) antibodies.