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Citations for abcam :
401 - 450 of 472 citations for Recombinant Rhesus PCSK9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The membrane was then incubated with appropriate primary antibodies: rabbit recombinant anti-MECP2 (ab253197, Abcam, 1:3000), rabbit monoclonal D51G7 anti-IRAK1 (#4504 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4° C overnight in 1:500 recombinant rabbit anti-HA tag (Abcam, AB236632) or rabbit anti-c-Fos (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant Anti-α-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (abcam, ab209538, 1:200). Lists of secondary antibodies used ...
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bioRxiv - Bioengineering 2024Quote: ... recombinant rabbit monoclonal primary antibody against TNF Receptor 2 [EPR1653] (dilution 1:200, ab109322, Abcam, Cambridge, MA) or normal rabbit serum (ab7487 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membranes were blocked with 5% milk (w/v) in TBST (TBS, 0.1% Tween 20) and were probed with horseradish peroxidase (HRP) conjugated anti-His antibody (Abcam, catalog # ab1187) or the C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 20μL of 1X phosphate buffered saline (PBS) and anti-6x His Alexa Fluor 647 (abcam, cat: ab237337) was added at a dilution of 1:100 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with an increasing concentration of unlabeled purified sema3A (0-100µg/ml) or recombinant human sema4D protein (0-50µg/ml) (Abcam) as competitive inhibitors ...
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bioRxiv - Cancer Biology 2019Quote: ... cultured osteoblasts or osteoclasts were treated with 25 ng/ml recombinant full-length human MMP-3 (Abcam, ab96555) in place of conditioned medium or in combination with conditioned medium.
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bioRxiv - Neuroscience 2020Quote: ... recombinant anti-M6PR (cation independent) antibody (ab124767) and rabbit polyclonal antibody VDAC1 were also purchased from Abcam (ab15895). The mouse monoclonal antibody against total LRRK2 (C-terminus ...
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bioRxiv - Synthetic Biology 2021Quote: ... Recombinant gene product was either detected with Goat anti-GFP conjugated to Alkaline Phosphatase (ab6661 Abcam, Cambridge, Massachusetts) at 1:5000 dilution or 1:5000 Rabbit Polyclonal anti-SARS-CoV2 RBD (Cat# 40592-T62 Sino Biological ...
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bioRxiv - Developmental Biology 2019Quote: ... and the other was embedded into type Ia collagen mixed with either 0.1 mg/ml laminin 211 (Biolamina, human recombinant laminin LN211) or 0.1 mg/ml collagen IV (Abcam, Natural Human Collagen IV protein (FAM ...
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bioRxiv - Immunology 2023Quote: ... and the recombinant Clec4F was detected using a goat anti-human IgG coupled to peroxidase (AbCam, Cambridge, UK). For competition ELISA ...
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bioRxiv - Molecular Biology 2023Quote: ... the plates (UltraCruz ELISA Plate sc-204463) were coated with purified recombinant human c-Myc protein (Abcam ab84132), diluted in three different concentrations (0.12 μg/ml ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used included 1:1,000 recombinant anti-ERG antibody (ab133264) and 1:5,000 anti-histone H3 antibody (ab1791) (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... Beads were resuspended in diluted kinase assay buffer containing 0.5 mM ATP and recombinant Aurora B kinase (AbCam). Kinase reactions were shaken at 30 °C for 10 mins at 1000 RPM and quenched with 4x LDS buffer (-EDTA ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were probed with the appropriate primary antibody: Recombinant Anti-Alpha-synuclein antibody [MJFR1] (1:2000 Abcam, ab138501) or β-Actin (1:5000 Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Both the supernatant and pellet (after washing with buffer F) were resuspended with SDS-loading buffer for SDS-PAGE and western blotting probed by mouse anti-His antibody (Abcam, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773, Goat pAb to rabbit IgG)) at a 1:5,000 dilution to detect RhaPS and His-tagged carrier proteins ...
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bioRxiv - Immunology 2021Quote: ... or 10 µg/mL recombinant full length murine MMP9 without additional chemical activation by p-Aminophenylmercuric acetate (ab39309, Abcam). After 4 hours at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... [APOE4/E4]-Ki astrocytes were incubated for 24 hours with the pro-inflammatory cocktail and different doses (0.5 and 20 µg/ml) of TAGLN3 as human recombinant protein (Abcam) prior to be analysed ...
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bioRxiv - Microbiology 2020Quote: ... The sequential sections from all collected tissues were incubated with a recombinant anti-Mouse IgG antibody [RM104] (ab190475, Abcam) performed as the negative control for the expression of viral antigen.
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bioRxiv - Immunology 2023Quote: Isolated human skin immune cells were treated with 10 µg/ml of recombinant HCMV-gB (Abcam, Cambridge, UK, ab43040) or 10 µg/ml of recombinant HCMV-gH (MyBioSource ...
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bioRxiv - Biophysics 2022Quote: ... The blocking buffer was removed and cells were incubated with recombinant anti-Lamin B1 primary antibody (ab229025, by abcam) diluted 1:250 (volume/volume ...
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bioRxiv - Neuroscience 2023Quote: ... Blockade of IL-1R was performed using the recombinant mouse IL1-RA protein (1-1000 mg/mL, abcam #ab283475).
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bioRxiv - Cancer Biology 2023Quote: ... CAFs were pre-treated prior to co-cultures for 2h with recombinant label-free 100nM Annexin-V (#ab157342, Abcam) or 100 nM Zn-DPA (Molecular Targeting Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in either recombinant primary rabbit anti-GFAP antibody (astrocytes, 1:500 in 2% BSA and 0.1% Triton [TBS]; Abcam), or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and then probed with primary antibody (mouse monoclonal anti-His from Pierce, 1:3000; rabbit monoclonal anti-β-Pol from Abcam, 1:2000) overnight ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315, Abcam, Cat.#ab6789, respectively). For HisTag purification ...
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bioRxiv - Immunology 2021Quote: ... and harvested into EDTA and used to measure plasma hemoglobin (plasma-Hb) and quantify soluble recombinant thrombomodulin (sTM-ELISA, ab209880, Abcam) and thrombin-antithrombin (TAT-ELISA ...
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bioRxiv - Cell Biology 2019Quote: ... was incubated with recombinant active PLK1 (0, 50 or 100 ng) or PLK1-kinase dead (100 ng) (purchased from Abcam or purified from sf9 insect cells as detailed above ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific; RRID:AB_1954821; Recombinant rabbit anti-phospho S129 alpha synuclein, 1:2000, Abcam 51253);the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and clone #124 (human + mouse selective antibody catalogue number ab256526) were chosen for recombinant antibody generation and are now commercially available from Abcam.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in a solution containing the Recombinant Anti-Iba1 antibody rabbit (ab178847, 1:500, abcam). After additional washes ...
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Immunology 2021Quote: ... AD-01 (NH2-QIRQQPRDPPTETLELEVSPDPAS-OH) (Almac Group, United Kingdom), ALM201 (NH2-IRQQPRDPPTETLELEVSPDPAS-OH) (Almac group, United Kingdom) and recombinant FKBPL protein (ab98117; Abcam). All experiments were carried out at 37°C in a humidified atmosphere of 95% O2/5% CO2.
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody produced in-house (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... recombinant IL-1-Ra at 40ng/mL (Biozol, #PPT-AF-2000-01RA ml and recombinant SLP-antibody at 5μg/mL (abcam, #ab191883), plasma diluted 1:20 of a patient with COVID-19 and high-titered IL-1-Ra-antibodies (Val 16 ...
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bioRxiv - Physiology 2022Quote: ... Samples were prepared using non-reducing sample buffer and were loaded (20 ug/well) onto the gelatin gel along with recombinant MMP9 (Abcam) and MMP2 (BioLegend ...
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bioRxiv - Plant Biology 2023Quote: ... CTR1-KD and MPK4 were analyzed by western blot using Recombinant Anti- Thiophosphate ester antibody (Abcam, Cat. Ab92570, 1:5000).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: recombinant rabbit anti-APOE mAb (1:300 IF; 1:50 IG; 1:500 WB; Abcam Cat# ab52607, RRID:AB_867704), mouse anti-HA-Tag mAb 6E2 (1:250 IF ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody recombinant anti-Fox2a antibody (ab108422) and secondary goat anti-rabbit IgG H&L HRP conjugated (ab6721) were obtained from abcam and used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Extracts corresponding to a known number of cells as well as recombinant 6xHis-PHB1 or 6xHis-PHB2 (Abcam, Cambridge, UK) were separated on 4-15% Mini-Protean® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: To treat cells with rPAI-1 we used recombinant active human PAI-1 (carrying stabilizing mutations N150K, K154T, Q319L and M354I, Abcam) in experiments on human airway epithelial cultures and in in-vitro complex formation assays ...
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bioRxiv - Genetics 2019Quote: ... The serums were diluted 100-fold and were analyzed by ELISA using Rabbit polyclonal to 6×His tag® (HRP) (Abcam, USA, code#ab1187) and the QuantaBlu™ NS/K fluorogenic substrate kit (Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were co-stained with the mouse monoclonal antibody for cardiac isoform Ab-1 Troponin at 1:100 dilution and recombinant rabbit anti-Desmin antibody at 1:70 dilution (Abcam #ab32362), or normal rabbit IgG antibody (Millipore Sigma #NI01 ...
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bioRxiv - Physiology 2022Quote: ... S100B meshwork was identified by a recombinant rabbit monoclonal anti-S100B antibody (1:250; clone EP1576Y; catalog # ab52642; Abcam, Cambridge, MA), and a chicken polyclonal anti-S100B antibody (1:300 ...