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Citations for abcam :
401 - 450 of 4824 citations for Recombinant Mouse Btc Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The membrane containing the purified proteins was probed with an anti 6X His Tag monoclonal antibody (Abcam; ab49746) conjugated to alkaline phosphatase at 1:2000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... the flow cell was incubated with a biotinylated anti-6X His tag antibody (cat #ab27025, Abcam, Cambridge, MA) (1 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... The following near infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were resolved by SDS-PAGE and western blots were performed with the anti-FLAG antibody to validate the immunoprecipitation of FLAG-tagged fl-SNED1 and with anti-phosphoserine (1 μg/mL; Abcam, ab9332), anti-phosphothreonine (1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... after twice washes then PE-labeled goat anti-human IgG-Fc antibody was added (1:5,000; Abcam) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies included: (1) goat anti-human IgG Fc (HRP) pre-adsorbed (catalog no. ab98624; Abcam); (2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% Tween 20) and then probed with horseradish peroxidase (HRP) conjugated anti-His antibody (1: 5,000) (Abcam, catalog # ab1187) or rabbit polyclonal serum ...
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bioRxiv - Bioengineering 2020Quote: Tissue levels of MMP12 in Hi-Myc and healthy FVB/NJ prostate homogenates were measured by ELISA according to manufacturer’s protocol (ab246540, abcam). Prostate homogenates were prepared as previously described ...
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bioRxiv - Biochemistry 2021Quote: ... and the purified protein band was also confirmed by Western blot analysis by using an anti-His antibody (Abcam).
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bioRxiv - Microbiology 2020Quote: ... In a first step the membranes were incubated either with 0.1 μg/mL Anti-6×His® antibody (Abcam) to detect EF-P ...
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bioRxiv - Microbiology 2023Quote: ... The blot was probed with an anti-His-tag antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cat No. ab1178). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... 0.05 % Tween20) and then incubated with horseradish peroxidase (HRP) conjugated anti-His antibody (1:10,000 dilution; Abcam, United Kingdom) in TBST with 5 % (w/v ...
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bioRxiv - Biophysics 2024Quote: ... Successful overexpression of AGO2 was confirmed using Western Blot analysis with an anti-6X His tag antibody (Abcam, AB18184).
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bioRxiv - Immunology 2022Quote: Recombinant human peroxiredoxins 1 and 6 (PRX1, PRX6) were purchased from Abcam (Abcam PLC, Cambridge, UK), while recombinant human β-defensin-1 and 2 (HBD-1 ...
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bioRxiv - Immunology 2021Quote: ... Labelling of recombinant proteins was carried out using APC and PE conjugation lightning-link kits (Abcam). When using biotinylated antibodies ...
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bioRxiv - Bioengineering 2020Quote: Human recombinant αSyn monomers and pre-formed fibrils were purchased from Abcam (Monomers; ab218819, Fibrils; ab218819), and were diluted in DopaNeurons Media to a final concentration of 4 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: We used the following antibodies: Recombinant monoclonal anti-Thiophosphate ester rabbit IgG [51-8] (Abcam, ab92570), monoclonal anti-GFP mouse IgG (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... were coated with 1% BSA or 20 nM of GST-fused recombinant human CCR5 (hrCCR5, Abcam) in PBS overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... incubated with primary antibodies for Paxillin (Recombinant Anti-Paxillin antibody [Y113], Abcam ab32084; 1:250 dilution) and Myosin (Phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained overnight at 4oC using Recombinant Anti-GC1q R antibody [EPR23238-6] (Abcam ab270033).
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bioRxiv - Cell Biology 2021Quote: ... Acetylated Histone 4K16 was detected using a recombinant rabbit antibody (Abcam, ab177191, GR284778-8, 1:400) and visualized using a donkey anti-rabbit Alexa Fluor 488 secondary antibody (Molecular Probes ...
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bioRxiv - Developmental Biology 2023Quote: 1 µg purified recombinant human PARD3 was incubated with 750ng of GST-CDK1/CyclinB1 (abcam; ab271456) in kinase buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated with either recombinant anti-CDKN2A/ p16Ink4a antibody (1:100; Abcam, catalogue #ab211542), sheep anti-calcitonin gene-related peptide (CGRP ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μg/mL recombinant rhesus monkey CD4 protein (for detecting Ibalizumab; Abcam, Cambridge, UK, cat.: ab208305), or 1:100 diluted goat anti-human IgG-Fc (for detecting total human IgG ...
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bioRxiv - Cell Biology 2023Quote: ... CENP-A (mouse mAb, AbCam, ab13939; mouse mAb, GeneTex, GTX13939), CENP-C (guinea pig pAb ...
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bioRxiv - Bioengineering 2022Quote: ... anti-mouse VCAM-1 (eBioscience) and anti-mouse Ki67 (Abcam). Slides were then washed three times in PBS-T and incubated with fluorochrome conjugated secondary antibodies for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... mouse monoclonal (Abcam Cat# ab16794 ...
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bioRxiv - Cell Biology 2024Quote: ... PDI (mouse;abcam).
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bioRxiv - Cell Biology 2023Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Biochemistry 2023Quote: ... A second block was performed (for mouse on mouse staining) using goat F(ab) anti-mouse IgG (Abcam) at 1:100 in PBST ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were washed again with PBSt and incubated with an anti-human Fc HRP-conjugated antibody (ab977225, Abcam). Development was performed with TMB solution and terminated with Stop solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... 3 ml of the adjuvant goat IgG anti-human IgM Fc (5 ng/ml) (by ABCAM, Cambridge, UK) was dissolved in human albumin and phosphate-buffered normal saline added to the remainder of the sediments and packaged in 1 ml vials of injectable solution with labels (See Supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg of a murine isotype-matched antibody to control for Fc receptor binding (murine IgG2a/κ; Abcam), or phosphate-buffered saline (PBS) ...
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bioRxiv - Synthetic Biology 2021Quote: ... for Im7 variants or 50 μL of 1:5,000-diluted HRP-conjugated anti-6×His tag antibody (Abcam, catalog # ab1187) for scFv-HER2 variants ...
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bioRxiv - Pathology 2022Quote: ... The pull-down reactions were analyzed by SDS-PAGE followed by Western Blot using anti-His (M20001; Abmart) and anti-GST (ab111947; Abcam) antibodies.
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed 2x with PBS followed by staining for surelight® APC conjugated anti-his antibody (Abcam, ab72579) for 30 min on ice ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant protein expression was verified by concentration determination using the Bradford method and by Western Blot analysis using the anti-His antibody (dilution 1:1000, Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Biochemistry 2023Quote: ... The samples along with the control were then loaded on 10% SDS-PAGE followed by immunoblot with anti-6x His or anti-Flag antibody (Abcam).
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... The plates were washed four times with PBST and were then incubated with 1:15,000 rabbit polyclonal 6x His-tag antibody conjugated to HRP (Abcam, Austria) for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-beta IV tubulin recombinant antibody conjugated with Alexa Fluor® 647 [EPR16775] (Abcam, cat# ab204034) was used at a 1:100 dilution.
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bioRxiv - Neuroscience 2022Quote: ... Recombinant Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (abcam, ab209538, 1:200). Lists of secondary antibodies used ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoids recovered from cell seeding were treated with 10 ng/ml recombinant rabbit integrin α2 (AbCam, ab181548) and 10 ng/ml mouse IgG1 integrin β1 (AbCam ...
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bioRxiv - Genetics 2023Quote: ... Then incubated at 4°C overnight with recombinant anti-NADPH oxidase 4 antibody (1:2000, ab133303, abcam), and mouse anti-GAPDH monoclonal antibody was used for normalization (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... according to manufacturer’s instructions using a recombinant rat monoclonal STAG1 antibody (Abcam ab241544, Lot:GR3334172-1; 1:200) after cooking 10 minutes at 110°C in antigen unmasking solution (Vector #H3301) ...
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bioRxiv - Genetics 2023Quote: ... The membrane was then incubated with appropriate primary antibodies: rabbit recombinant anti-MECP2 (ab253197, Abcam, 1:3000), rabbit monoclonal D51G7 anti-IRAK1 (#4504 ...