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Citations for abcam :
4201 - 4250 of 10000+ citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Proteins were detected with bis-tris western blotting for U1A (rabbit, Abcam, 1:200), and slot blotting for WDR5 (rabbit ...
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bioRxiv - Cancer Biology 2019Quote: ... Non-specific antigen blocking was performed using Protein Block Solution (#ab156024, Abcam, Cambridge, MA) incubation for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Full length human GST-tagged P53 protein was purchased from Abcam (Catalog number: ab43615), and His6-CREB1 was purchased from Origene (Catalog number ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined by Coomassie assay according to the manufacturer’s instructions (Abcam: #ab119211). For immunoblotting ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by alkylate the protein with 1.5 µl of the 50mM PNBM stock (Abcam) at room temperature for 2hrs ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleaning of lysate was carried out using blocked Protein G sepharose (ab193259, Abcam). Blocking of Protein G sepharose was carried out by using 500ng/ul yeast tRNA and 1mg/ml RNase-free BSA ...
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bioRxiv - Physiology 2022Quote: ... Protein homogenates were analyzed for abundance of phosphorylated 4-hydroxynonenal (4-HNE; ab48506; Abcam), GPx4 (ab125066 ...
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bioRxiv - Biochemistry 2023Quote: ... The gel was stained with 25 mL of InstantBlue Coomassie Protein Stain (Abcam, ab119211) for 15 min at RT and protein bands of interest were excised ...
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bioRxiv - Cancer Biology 2023Quote: ... GDF15 (Protein-tech, 27455-1-AP; 1:500) and FOXP3 (Abcam, ab215206; 1:1000) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then stained with rat anti-myelin basic protein (#ab7349, Abcam, 1:100), rabbit anti-GFAP (#ab7260 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein bands are visualized by re-staining the same gels by Coomassie staining (AbCam) overnight ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP) for astrocytes (1:500, ab279290, Abcam, UK), rabbit anti-neuronal nuclei (NeuN ...
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bioRxiv - Cancer Biology 2023Quote: ... and 100 ng/ml of active mouse recombinant Ifng protein (Abcam, catalog number: 259378) for 3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble nuclear and insoluble nuclear proteins were isolated using a commercial kit (#ab219177, Abcam) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... biotin and biotin-binding proteins was blocked with Avidin/Biotin Blocking Kit (abcam, ab64212) in those sections where botinylated secondary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: Immunoprecipitation assays were performed using the mitochondrial protein IP kit from Abcam (catalog # ab239710) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 96-well MaxiSorp round bottom U96 Nunc plates were coated overnight at 4°C with 50 µl of 50 mM sodium phosphate (pH 7.5) and 10% glycerol buffer containing 0.5 µg of goat anti-GFP IgG (Abcam; Ab6673). Wells were washed once with 200 µl of TCS buffer prior to immunoprecipitation ...
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bioRxiv - Developmental Biology 2019Quote: Zebrafish were housed from 24 to 72 hpl in system water containing 10 µM TAE684 (Abcam, ab142082, Cambridge, UK) constructed from a 20 mM stock solution in 0.1% dimethylsulfoxide ...
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bioRxiv - Neuroscience 2020Quote: ... Sections containing Cg1 and M2 were first incubated in citrate buffer (diluted from 10x citrate buffer, pH 6.0; ab64214, Abcam) for 45 min at 95°C ...
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bioRxiv - Physiology 2020Quote: ... rinsed in PBS 2×10min and then incubated in blocking solution containing 5% normal goat serum (NGS, Ab7481, Abcam), 0.5% Triton (T8787 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4° C with a solution containing 5% goat serum (GS) and anti-cFos (rabbit; 1:5000, AB_190289, Abcam) diluted in PBST ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections were incubated on a shaker for 48 h at 4 °C with primary antibodies in the blocking buffer containing 0.1% Triton X-100 at the following dilutions: rabbit anti-TH (1:1000, Abcam), chicken anti TUJ1 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells are resuspended as a single-cell suspension at densities of 750K-1M cells/mL in mTeSR1 containing 10μM ROCK inhibitor Y27632 (Abcam). 200μL of cell suspension is then pipetted onto prepatterned dishes and allowed to settle for 15 minutes before adding 1mL of mTeSR1 and allowing cells to settle for 10 additional minutes ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 10 min in 0,3% TritonX PBS containing 1μg/mL DAPI and 1:100 Phalloidin-rhodamine (Abcam ab235138), rinsed and mounted in PBS solution ...
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bioRxiv - Neuroscience 2024Quote: ... N2a cultures were first incubated for 16-20 h in EMEM containing 1% pen-strep and 10% FBS supplemented with 0.5 µM staurosporine (ab146588; Abcam) then labeled with the pHrodo Red Cell Labeling Kit for Incucyte (4649 ...
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bioRxiv - Microbiology 2024Quote: Cell pellets were washed in cold PBS and lysed with buffer containing 1% NP40 and protease inhibitor cocktail (Abcam). Whole cell lysates were obtained by centrifugation and removal of cell debris ...
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bioRxiv - Bioengineering 2023Quote: ... separate slides were incubated in a blocking solution containing 0.3% Triton X-100 and 10% normal goat serum (Abcam) in 1xPBS + 100 mM CaCl2 + 100 mM MgCl ...
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bioRxiv - Pathology 2023Quote: ... and then incubated for 1 hour in PB solution containing 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). The sections were then incubated with the primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... then switched to either normo-glucose or glucose-deprived media containing the viability dye DRAQ7 (Abcam ab109202, 1:100). Immediately following the media change ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with PBS and lysed with ice-cold lysis buffer containing NP-40 (2%, ab142227, Abcam), Triton X-100 (2%) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated at 37°C for 30 min with complete DMEM containing 5 µM Zinpyr-1 (Abcam, ab145349), Hoechst 33342 (0.2 µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed with PBS and fresh medium was added containing 1 µg/ml Hoechst 33342 (Abcam, Cat# ab228551) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: Sections were pre-incubated with a blocking PBS buffer containing 0.5% Triton X-100 and 10% normal bovine serum (NBS; ab7479, Abcam) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... All slices were incubated overnight at 4 °C in blocking solution containing either 1:1000 anti-GFP (ab13970, Abcam) to reveal dLight1.1-expressing cells in NAc ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was washed again three times in PBS and mounted for imaging with medium containing DAPI (ab104139, Abcam). The number of c-Fos positive nuclei in each ROI was quantified using ImageJ software ...
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bioRxiv - Cell Biology 2020Quote: Antibodies used in the study were: (WB: western blot, IF: immunofluorescence, FC: Flow cytometry): Mouse monoclonal antibodies raised against SNX27 antibody (clone 1C6, Abcam, Ab77799, WB), GFP (clones 7.1/13.1 ...
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bioRxiv - Microbiology 2021Quote: ... FliC and SigA proteins on the gel were detected with anti-FliC and anti-SigA primary antibody (1:1000) and horseradish peroxidase-conjugated secondary antibody goat anti-mouse (Cat: ab97023, IgG, 1:3000, Abcam, Cambridge, MA) by Western blot ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Cells were again washed in DPBS and incubated for 1 h at room temperature with Alexa Fluor® 488-conjugated secondary antibody (goat polyclonal antibody to rabbit IgG, 1:500, Abcam, Ab15077). Nuclei were counterstained with 4’,6’-diamidino-2-phenyliondole (DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody (goat polyclonal antibody to rabbit IgG, 1:500, Abcam, Ab150077). Nuclei were counterstained with DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... Phosphorylation of Rab8a at residue T72 by LRRKRCKW was monitored by western blot using a commercially available antibody (Abcam antibody MJF-R20) as previously described31,73.
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with appropriate primary antibodies prepared in blocking buffer against glucagon (mouse antiglucagon antibody, Cat # ab10988, Abcam; 1:1000 or rabbit anti-glucagon antibody, Cat# ab92517, Abcam; 1:25), Stathmin-2 (goat anti-SCG10 antibody ...
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bioRxiv - Bioengineering 2021Quote: ... Asialoglycoprotein receptor 1 (ASGPR1)/HL-1 polyclonal antibody (ASGR1PAB) (ab49355) and recombinant ACE2 monoclonal antibody (ACE2MAB) (ab108252) were purchased from Abcam (Cambridge, MA). NPHS2 (podocin ...
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bioRxiv - Plant Biology 2020Quote: ... The following antibodies were used for ChIP assays: TEN antibody, anti-H3K56ac (active motif, Cat. #39282) and anit-H3K122ac (Abcam, Cat. #Ab33309) (Supplementary Table 9) ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with a rabbit α-RSV antibody (1:300) [36] and a Cy3-conjugated α-rabbit secondary antibody (1:100, Abcam). DAPI was added at 5 μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and further incubated with rabbit monoclonal anti-DIMT1 antibody (1:1000 dilution) and Rabbit polyclonal anti-OXPHOS (1:250 dilution antibody; both from Abcam, Cambridge, UK). Tubulin antibody (1:5000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibodies used in the present study are listed as follows: anti-MTA1 polyclonal antibody (1:2000 dilution; Abcam, Cambridge, UK), anti-S100A4 polyclonal antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and were then incubated overnight at 4 °C with following primary antibodies diluted in TBST: Anti-alpha Actinin 4 rabbit monoclonal antibody (Abcam, Cat # ab108198), Anti-Vimentin mouse monoclonal antibody (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... CST), anti-Lamin A/C mouse monoclonal antibody (Abcam, Cat # ab8984, Anti-non-muscle Myosin IIA rabbit polyclonal antibody (Abcam, Cat #ab75590), Anti-non-muscle Myosin IIB mouse monoclonal antibody (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... Von Willebrand factor (VWF, sheep IgG) primary antibody and cluster of differentiation 68 (CD68, rabbit IgG) primary antibody were purchased from Abcam (Cambridge, Ma). Human PDGF-BB standard ABTS (enzyme-linked immunosorbent assay ...