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Citations for abcam :
3951 - 4000 of 10000+ citations for Streptavidin ZAP Antibody Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were placed on a 45 µL drop of primary antibody solution consisting of 1% BSA in PBS with primary antibodies as follows: GLUT4 (Abcam, 33780) 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... rinsed with 1% BSA 3X and processed for antibody staining as detailed above with a sheep polyclonal antibody to BrdU (1:100—Abcam, #ab1893), then Alexa Fluor Donkey anti-sheep 594 secondary (1:250—Life Technologies #A-11016) ...
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bioRxiv - Neuroscience 2019Quote: ... Followed by incubation with goat anti-rabbit lgG H&L (Alexa Fluor® 594) preadsorbed secondary antibody (1:500 diluted in antibody dilution buffer, Cat #: ab150088, Abcam) for 2 hrs at room temperature in dark ...
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bioRxiv - Bioengineering 2019Quote: ... and processing the samples for immunocytochemistry using mouse monoclonal Tf antibody (Serotech 9100-1057) and rabbit polyclonal TfR antibody (Abcam ab84036) diluted 1:250 ...
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bioRxiv - Microbiology 2020Quote: ... The following near infra-red (NIR) fluorescent secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then blocked at room temperature for 1 hour in 10% heat-inactivated goat serum and then stained with the following affinity-purified primary antibodies overnight at 4°C: anti-β-Catenin antibody (1:500; ab6302, Abcam), anti-Cdh1 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... were resolved by SDS-PAGE and the target proteins were detected with Western blot (WB) using the antibodies as follows: GAPDH Rabbit monoclonal antibody (ab181602, Abcam, UK), SLC40A1 Rabbit polyclonal antibody (ab58695 ...
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Cell Biology 2019Quote: ... The primary antibodies used in this study were rabbit polyclonal antibody against FAK phosphorylated at tyrosine 397 (pFAK-Y397) (Abcam, ab4803), rabbit monoclonal antibody paxillin (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... the sections were stained with 7D2 monoclonal antibody (1:500 dilution, laboratory preparation) and CD68 antibody (1:500 dilution, Abcam, ab201340) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Wash buffer was removed by placing the samples on a magnet and antibody buffer containing 0.0125% of digitonin and GATA1 antibody (Abcam, 1:100) or rabbit gG control antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed and fixed with methanol and probed for rabbit α-synuclein primary antibody (26283, CST) and clathrin primary antibody (ab129326 abcam) for 1 h at RT in humid chambers ...
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bioRxiv - Biochemistry 2022Quote: ... The following antibodies were used for staining: primary antibodies for 1 h at room temperature: CENP-A (1:500, Abcam ab13939), GFP (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... a mouse ascites antibody), 14A10 (anti-RSV L, a mouse ascites antibody) and RSV 5H5 (anti-M2-1, ab94805) (Abcam, UK). All primary antibodies were used at 10 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used were rabbit anti-Zfp36l1/Zfp36l2 (this antibody recognizes Zfp36l1 at 37kDa and Zfp36l2 at 51kDa) (Abcam, 1:500) and mouse anti-βactin (Millipore ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... MHC molecules were visualized on the membranes with polyclonal rabbit anti-HA antibody as primary antibody (ab9110, Abcam, Cambridge, United Kingdom) and alkaline phosphatase-conjugated anti-rabbit serum from goat as secondary antibody (1706518 ...
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bioRxiv - Neuroscience 2022Quote: ... with constant agitation and subsequently with the primary antibody (Rabbit anti somatostatin, 1:500, BMA Biomedicals; Rabbit anti parvalbumin, 1:1000, Swant antibodies; Chicken anti GFP, 1:000, Abcam; Mouse anti-FLAG ...
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bioRxiv - Bioengineering 2021Quote: The following primary antibodies were used for the immunostaining approach of LR-ExM: rabbit anti-Clathrin heavy chain antibody (Abcam, ab21679), rat anti-alpha-Tubulin Antibody ...
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bioRxiv - Microbiology 2020Quote: ... Then the sections were incubated with Rabbit anti-EEEV antibody (USAMRIID, 1:1000) and chicken anti-NeuN antibody (Abcam, 1:25), or chicken anti-GFAP (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used in this study were as follows: monoclonal mouse antibody raised against mouse SOX9 at 1∶:500 dilution (AbCam, ab26414), polyclonal rabbit antibody raised against mouse STRA8 at 1∶:500 dilution (AbCam ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cultures were blocked with 10% fetal bovine serum for 2 hours and then co-labeled overnight with different primary antibodies: chicken polyclonal anti-MAP2 antibodies (1:2,500) (# ab92434, Abcam, Cambridge MA), rabbit monoclonal anti-Iba1 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescent staining with COL-IV and ZO-1 antibodies was performed using anti-collagen IV rabbit polyclonal antibody (Abcam, ref ab6586), anti-ZO1 monoclonal antibody (ThermoFisher ...
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bioRxiv - Bioengineering 2020Quote: ... The slices were then stained overnight at 4 °C in block solution containing beta III tubulin (β3-Tubulin) antibodies for labeling of neurons (1:100 ab201740 Anti-beta III Tubulin antibodies conjugated with Alexa Fluor® 594, Abcam). The slices were then washed in PBS (twice ...
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bioRxiv - Molecular Biology 2021Quote: ... Western-blot analysis was performed using the following antibodies: polyclonal anti-VLP antibody ([81]; 1:10,000) and monoclonal anti-Pab1 (Abcam; 1:1000). Quantification of signals was performed using the ImageJ software.
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bioRxiv - Microbiology 2020Quote: ... The following near infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG ...
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bioRxiv - Cancer Biology 2019Quote: ... using approximately 1×107 SKmel28 clonal CRISPRi cells per assay and 5 μg of the following antibodies: anti-SOX10 rabbit monoclonal antibody (Abcam; ab155279), anti-Histone H3K27ac rabbit polyclonal (Active Motif ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Genetics 2020Quote: ... followed by incubation in solution containing 1:50 polyclonal anti-NCOA3 antibody (anti-SCR3 antibody - ChIP Grade, Rabbit Polyclonal, ab2831, Abcam Inc.) diluted in 0.1% triton X-100 and 4% BSA ...
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bioRxiv - Physiology 2021Quote: ... Baltimore PKD Center), anti-PC-2 monoclonal antibody (3374 CT-1 414, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Physiology 2021Quote: ... arteries were incubated overnight with anti-PC-1 monoclonal primary antibody (E3 5F4A2, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The slides then underwent heat mediated antigen retrieval followed by incubation with primary antibody - anti-Collagen 1 antibody-3G3- (Abcam - ab88147) and the secondary antibody - AffiniPure Goat Anti-Mouse IgG (Jackson Immunoresearch Lab Inc - Code - 115005003) ...
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bioRxiv - Cell Biology 2022Quote: The following antibodies were purchased then stored according to the vendors specifications: NeuN antibody conjugated to Alexa Fluor 568 (Abcam, Ab207282).
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bioRxiv - Bioengineering 2022Quote: ... The primary antibody (αSMA [D4K9N, Cell Signaling Technology, Beverly, MA, USA] or Recombinant Anti-GFAP antibody [Abcam, ab207165, Cambridge, MA, USA]) was diluted 1:150 in AbScale solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133; SOX11, Sigma #HPA000536 or HNRNPH1 Abcam #ab154894) was added for incubating on nutator overnight at 4□°C ...
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bioRxiv - Developmental Biology 2022Quote: ... β1-integrin blocking antibodies at 100 μg/mL (MilliporeSigma; # MABT821) or mouse monoclonal (P5D2) β1-integrin blocking antibodies at 50 μg/mL (Abcam; ab24693).
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bioRxiv - Cell Biology 2022Quote: ... Leica Bond Polymer anti-rabbit HRP was used, for the hamster antibody and the mouse antibody, rabbit anti-Hamster (Novex, A18891) and rabbit anti-mouse (Abcam, ab133469) secondary antibodies were used as linkers before the application of the Leica Bond Polymer anti-rabbit HRP ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoblotting was done overnight at 4°C with the following antibodies at the appropriate dilutions: TRPC6 antibody (Abcam ab228771, 1:1000). The blots were washed the next day and incubated with Goat anti-Rabbit-HRP secondary antibody (Cat # 31460 ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies: Anti-CDK1 (phospho T161) + CDK2/CDK3 (phospho T160) antibody [EPR19546] (ab201008) at a dilution of 1:2000 (Abcam, USA) and β-Actin Antibody (4967S ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared coverslips were incubated with the following primary antibodies prepared in mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:500) or monoclonal anti-TOM20 antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (50 μm) of skin were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The samples were incubated with 10 μg of following antibodies (1:100 dilution): anti-H3K27ac antibody (ab4729, Abcam, Abcam, Cambridge, MA) at 4°C overnight and then with protein A agarose/salmon sperm DNA slurry (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... was used for AnkB localization in cortical neurons in culture by immunofluorescence staining with mouse monoclonal anti-AnkB antibody 33-3700 and AF-594-conjugated secondary antibodies (Abcam, #ab245992). STED images were acquired using a Leica SP8 STED confocal microscope with Lightning deconvolution in the UNC Neuroscience Research Center.
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation of the cleared lysates was performed overnight at 4C with 5μg of anti-VDR antibody or anti-RXRα monoclonal antibodies (Abcam – Table 2). The beaded complexes were washed several times with NP-40 lysis buffer ...