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Citations for abcam :
351 - 400 of 402 citations for Methacrylic acid N hydroxysuccinimide ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... Phosphorylation of MSD2 was detected by anti-phospho Ser/Thr and anti-phospho Tyr primary antibodies (Abcam, cat. n. ab117253 and RM111) both diluted to 1:3,000 in TBST with 3% (w/v ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... Thermo Fisher, N-21479, donkey anti-rabbit 488, Abcam, ab150061, donkey anti-mouse 568, Abcam, ab175700, donkey anti-goat 647, Abcam, ab150135) in concentration of 1:500 were applied ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in antibody buffer (1%BSA in 1xPBS+0.02% Tween20) for 1 h at RT and stained overnight (o/n) with primary antibody anti-Tp63 (Abcam, clone 4A4, #ab735) diluted 1:1000 at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
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bioRxiv - Neuroscience 2021Quote: Young adult male mice (6-8 weeks old) were administered an intraperitoneal (i.p.) injection of kainic acid (KA, Abcam) dissolved in 0.9% saline solution at 20 mg/kg body weight ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration in endothelial cell lysates were measured using bicinchoninic acid assay (BCA) Protein Assay Kit (Abcam, Cambridge, UK). NOS production was measured indirectly by Griess reagent in samples using Nitric Oxide Synthase (NOS ...
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: The concentration of free fatty acids in the adipocyte-CM was quantified using an ELISA assay kit (ab65341; Abcam). The levels of adipokines in the plasma of WT and BaKO mice were measured using the Mouse Adipokine Array Kit (ARY013 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Staining was completed using Mayer hematoxylin and 0.2 % eosin (Histolab) or Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (Abcam). Imaging of the slides was performed using an Olympus BX60F microscope with an SC50 camera (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... Prior to DENV-2 infection cells were pre-treated with 1 µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... B16F10 tumor cells were expanded and then supernatant was tested for lactic acid production as per the manufacturer’s instructions (Abcam). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Nucleic acid samples were treated with or without RNase H and then incubated with 5μg of S9.6 antibody (Abcam, USA) or mouse IgG (Beyotime ...
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bioRxiv - Immunology 2021Quote: ... The wells were washed six times with PBS-T 0.05% and further incubated with 100 µL (1:28000) of Goat Anti-Syrian Hamster IgG conjugated to HRP (cat. n° ab6892, Abcam, Cambridge, MA, USA) diluted in 1% Difco Skim Milk in PBS-T 0.05% for 1h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Cell Biology 2022Quote: ... milk and the membranes incubated at 4°C overnight with rabbit Anti-Annexin A1/ANXA1 antibody monoclonal antibody [EPR19342]-BSA and Azide free (1: 25000, Abcam, Cat. N. ab222398), ALIX (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were differentiated in a medium containing 1% Fetal Calf Serum supplemented with 10 μM trans-Retinoic acid (Abcam) for 5 days ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were differentiated in a medium containing 1% Fetal Calf Serum supplemented with 10 μM trans-retinoic acid (Abcam) for 5 days ...
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bioRxiv - Biophysics 2020Quote: ... which was followed by the media replacement with a fresh media containing FCS reduced to 1% and supplemented with 10 μM trans-Retinoic acid (Abcam) for 5 days ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody cocktail consisted of mouse anti-glutamic acid decarboxylase 65 (GAD65, abcam, Cat# ab26113, RRID: AB_448989, 1:500), mouse anti-glutamic acid decarboxylase 67 (GAD67 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-DCLK1 (anti-DCMKL1) antibody against amino acids 679-729 near the C-terminus of DCLK1 (Cat. No.: ab106635; Abcam), mouse anti-Src Mab327 antibody (RRID ...
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bioRxiv - Physiology 2020Quote: ... treated with ethylenediamine tetraacetic acid (EDTA) buffer pH 8 and incubated with rabbit anti-p16 antibody (1:300, Abcam ab211542) or anti-p19 antibody (1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... for 6 or 20 hours before inducing degradation of GRASP55 and/or GRASP65 by addition of 500 μM indole-3-acetic acid (IAA, Abcam). The Golgi ribbon was disassembled into single stacks by treatment with 3 μg/ml nocodazole (EMD Millipore ...
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bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
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bioRxiv - Immunology 2023Quote: ... or Picro Sirius Red Solution followed by Acetic Acid as indicated by the manufacturer (Picro Sirius Red staining, Abcam, ab150681). The severity of fibrosis was assessed on whole lungs using the Ashcroft modified method which assigns 8 grades (0 ...
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Membrane fluidity was measured as previously described (136) using a membrane fluidity kit that quantifies the fluorescence of a lipophilic dye (Pyrenedecanoic acid) (Abcam). Pyrene decanoic acid exists in monomeric and dimeric states within membranes ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were treated with 7-Chlorokynurenic acid (7-CKA) (final concentration 50 μM; 1 μL stock solution in DMSO in 1 mL media; ab120024; Abcam) and D-AP5 (final concentration 100 µM ...
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bioRxiv - Cell Biology 2020Quote: ... NADH levels in N13 WT and N13 R cells were measured using a NAD/NADH assay kit (NAD/NADH Assay Kit, Colorimetric, cat n. ab65348, Abcam, San Francisco, CA, USA), according to manufacturer’s indications.
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bioRxiv - Immunology 2021Quote: ... the monolayer was incubated with Donkey Anti-Rabbit IgG H&L-Alexa Fluor® 594 (1:250) (cat. n° ab150072, Abcam, Cambridge, MA, USA) and Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Cell Biology 2021Quote: ... was measured by reacting with thiobarbituric acid (TBAR) to generate a MDA-TBAR adduct according to the manufacturer’s instructions (Abcam, Cambridge, MA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in Acetic Acid solution (Picrosirius Red Stain Kit, Abcam, ab150681) and imaging ...
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bioRxiv - Pathology 2023Quote: ... CEBP/α (diluted 1:400, Huabio, EM1710-20, CA) and fatty acid-binding protein (FABP4, diluted 1:200, abcam, ab92501, UK) overnight at 4□ ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μl dissolved metabolites or collected medium or standard was mixed with 20 μl AQC (#ab145409, abcam, amino acid derivatization agent) and 120 μl borate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies raised against α-syn C-terminus (Mouse anti α-syn, Amino Acid (AA) 124-134 (1:1,000) and ab1903 (Abcam); Mouse anti α-syn 4B12 ...
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bioRxiv - Immunology 2023Quote: ... and nuclear proteins were extracted for use in western blotting with an antibody targeting amino acids 250-350 at the C-terminus (Abcam, ab150743) of the Foxp3 protein as previously described ...
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bioRxiv - Developmental Biology 2023Quote: ... Lung MDA content was measured by centrifugation of freshly homogenized mice lung tissue and incubating the supernatants with thiobarbituric acid (Abcam, ab118970) at 95°C for 1 hour followed by cooling to RT and measuring absorbance on a SpectraMax® i3x plate reader at 532 nm according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections at the level of the PnC of GlyT2-eGFP mice injected with pAAV DJ-CamKIIα-mCherry in the CeA (N=6 mice) were incubated with a chicken anti-mCherry (1:1000, Abcam, catalog# ab205402, lot# GR225123-3) and a rabbit anti-PSD95 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Performance-matched pairs were received systemic injections of either the pharmacological class I HDAC3 inhibitor RGFP966 (Abcam Inc., ab144819; 10 mg/kg; s.c.; N = 12) or vehicle solution (N = 9 ...
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bioRxiv - Genetics 2020Quote: ... and incubated with rabbit polyclonal anti-PEA-15 (C-terminal amino acids 93-123 of Human PEA15) antibody ab135694 (Abcam, Cambridge, UK) at a concentration of 1:100 and anti-GAPDH antibody (MAB374 ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocyte cell culture samples were probed for glutamic acid decarboxylase (GAD) 65-67 using rabbit anti-GAD 65-67 (Abcam, catalog #183999) with the corresponding secondary HRP-conjugated antibody and re-probed for β-actin (as described above) ...