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Citations for abcam :
351 - 400 of 409 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in YE4S at 32°C for at least 8 generations and treated for 15 minutes with 30μM 3-Brb-PP1 (3-[(3-Bromophenyl)methyl]-1- (1,1-dimethylethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (Abcam), while control culture was treated with equal volume of methanol ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies (anti-Cd31, goat polyclonal, R&D Systems #AF3628, 1:100; anti-VEGFA, rabbit monoclonal, abcam #ab52917 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with a solution of D-Wash buffer and Rabbit-antimouse IgG (1:200, Abcam, ab6709), followed by a wash step ...
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bioRxiv - Physiology 2024Quote: ... GDF15 and FGF21 were measured in mouse serum by ELISA kits (Abcam ab216947 and R&D Systems MF2100). TG and FAA in mouse serum were analyzed by Cayman (10010303 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained with anti-FAP (R&D AF3715) 1:100 or anti-HLA-DR+DP+DQ (Abcam ab7856) 1:200 overnight at 4 oC ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed again (0.05M TBS pH 7.4) and incubated with rabbit anti-D-serine antibody (1:1000, Abcam, #6472) with 0.05mM L-serine-BSA-glutaraldehyde conjugate overnight at RT ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with the fluorescent labelling reagent Fluo-4 AM (at 1μM in D-MEM) for the detection of intracellular calcium (GR3332801, Abcam) for 1 hour at 35°C ...
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bioRxiv - Neuroscience 2022Quote: ... OTX2 (Goat, 1:300, R&D: AF1979), Nestin (Mouse, 1:100, Invitrogen: MA1110, MAP2 (chicken, 1:500, Abcam: ab5392), MAP2 (Mouse ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:1000, PGRN (sheep, R&D AF2557) diluted 1:400 and Cathepsin B (CTS B)(rabbit, Abcam ab214428) diluted 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Resulting chromatin fragments were immunoprecipitated with antibodies against STAT5 (R&D AF2168, 10 μg/IP) or IgG control (Abcam ab6709 ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells under respective conditions were stained with cathepsin D and L kits (Abcam, Cat# ab65302 and ab270774) following company protocols ...
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bioRxiv - Neuroscience 2024Quote: ... and D-AP5 (final concentration 100 µM; 1 μL stock solution in water in 1 mL media; ab120003; Abcam) and incubated for 80-90 minutes at 37°C in humidified 5% CO2 ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Cells were incubated in antibody binding solution (5% NGS and 0.1% Triton X-100 in D-PBS) containing LaminB1 (Abcam) or SON (Atlas Antibodies ...
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bioRxiv - Genomics 2024Quote: ... cells were incubated with a solution of D-Wash buffer and Rabbit-anti-mouse IgG (1:200, Abcam, ab6709), followed by a wash step ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Immunology 2021Quote: ... monoclonal rat anti-human somatostatin (dilution 1:100; MAB2358, R&D) and monoclonal rabbit anti-chromogranin (dilution 1:400, ab15160, Abcam) ON ...
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bioRxiv - Neuroscience 2020Quote: ... after which tissues were washed in sterile PBS and incubated overnight with Rabbit anti-HuC/D primary antibody (1:750; Abcam) at 16°C with constant shaking ...
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bioRxiv - Cancer Biology 2022Quote: Cytotoxicity and apoptosis were assessed using the Annexin V-CF Blue 7-Aminoactinomycin D (7-AAD) Kit (Abcam Cat# AB214663) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Blocking buffer was aspirated and cells were incubated for 45 min with primary antibody (ACE2 R&D #MAB9332 and/or GM130 Abcam #ab52649 ...
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bioRxiv - Immunology 2021Quote: ... goat anti-human/mouse myeloperoxidase (1:100, AF3667, R&D) and rabbit anti-mouse histone H3 (citrulline R2 + R8 + R17) (1:200, ab5103, Abcam), diluted in blocking buffer were added and incubated for 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SMO (Santa Cruz sc-166685, mouse IgG2a in IF 1:200; Abcam ab236465 for Figs. 5B, D and E), anti-PTCH1 (Abcam ab53715 ...
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bioRxiv - Developmental Biology 2022Quote: ... Following primary antibodies (αTFAP2A, Abcam ab108311; αTFAP2B, Abcam ab221094; αLEF1, Cell Signaling, 2230; αSOX2, R&D Systems, AF2018; αPITX1, Abcam ab70273 ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in 1:250 dilution of anti-ERK3 antibody (mouse Cat# MAB3196, R&D or rabbit Cat# ab53277, Abcam) in blocking solution for 1 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were stained with sheep anti-human CD31 (R&D) at a 1:40 dilution and mouse anti-human alpha smooth muscle actin (Abcam) at a 1:200 dilution for 2 h at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... and MBL contained in human ALF samples to M.tb was determined by indirect ELISA using primary monoclonal antibodies (mouse anti-human SP-A, mouse anti-human SP-D, mouse anti-human C3/C3b, and mouse anti-human MBL; all from Abcam) and secondary antibodies (donkey anti-mouse HRP and goat-anti rabbit HRP ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking solution was replaced with ice-cold blocking solution (3% BSA in D-PBS) containing conjugated antibodies (Alexa 647 anti-p-Ser65-Ub [CST, 62802, 1:1500], Alexa 488 anti-HSP60 [Abcam, ab128567 ...
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bioRxiv - Systems Biology 2024Quote: We modified the canonical Cell Painting assay to remove the cytoplasmic RNA and nucleoli stain for a stain that specifically marks cleaved N-terminal Gasdermin D (Anti-cleaved N-terminal GSDMD Rabbit Monoclonal, Abcam).27 Additional Cell Painting organelles and stains included Nuclei (Hoechst 33342) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rotated for 2 hours at 4 °C in 200 μL D-Wash with 1:200 LMNB2 (Abcam, ab8983) followed by a wash step with D-Wash ...
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bioRxiv - Cancer Biology 2024Quote: The apoptosis assay was performed using FACS using Annexin V–CF Blue/7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit (ab214663, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Genetics 2020Quote: ... Freshly isolated fibroblasts lysates were used to measure cathepsin D activity using a fluorometric plate reader assay per manufacturer instructions (abcam ab65302).
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bioRxiv - Neuroscience 2020Quote: ... The L-type calcium channel blocker isradipine and the NMDA-glutamate receptor inhibitor D-AP5 were obtained from Abcam (Cambridge, MA). Stock solutions of MgCl2 (1M ...
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bioRxiv - Plant Biology 2024Quote: ... blots were stripped and re-probed with anti-HSC70 monoclonal antibody (Enzo #ADI-SPA-818-D) at 1/5000 dilution followed by Rabbit Anti-Mouse IgG H&L (HRP) secondary antibody (AbCam ab6728) at 1/10000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... blocked with 5% BSA for 1 h, incubated with anti-Sparc (1:100, R&D, AF942) or anti-Collagen I (1:200, Abcam, ab21286) or anti-Ocn (1:200 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated with primary antibodies O/N at 4°C (Rabbit anti-human Lyve-1, Fitzgerald; Goat anti-murine Lyve1, R&D AF2125; rabbit anti-CD31, abcam Ab28364) and washed three times in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... tadpoles were stained in primary antibody solution (1 mL CAS-Block containing 10 μL anti-acetylated tubulin (T7451) and 10 μL anti-HuC/D (Abcam ab184267)) for 2 days at 4 ℃ ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Antibodies in fresh 5% NFDM were added (Mouse monoclonal GAPDH 1:10,000; Goat polyclonal ACE2 [R&D Tech AF933] 1:200; Rabbit monoclonal BRD2 [abcam 197865] 1:5,000) and incubated at 4 °C for at least 16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Luk A and Luk B were heterologously expressed and purified as described elsewhere.22 Leukocidins E (ab190128) and D (ab190423) were purchased from Abcam (Berlin, Germany).
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bioRxiv - Neuroscience 2022Quote: ... For AMPA receptor-mediated miniature excitatory postsynaptic currents (mEPSCs) the aforementioned bath solution contained additionally 10 μM D-APV (Abcam, Cat# ab120003), 10 μM bicuculline methiodide (BMI ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used: anti-SOX2 diluted 1:500 (R&D AB2018, mouse) and anti-NR2F1 diluted 1:1000 (Abcam Ab181137, rabbit). Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... MockIP and WCL were aliquoted into wells in a 96-well plate and assayed using a fluorometric Cathepsin D activity assay kit (Abcam. Cat# Ab65302) that is based on quantifying the hydrolysis of the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 ...
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bioRxiv - Neuroscience 2020Quote: ... Afterward the sections were incubated with 1:100 diluted mouse polyclonal anti-GFAP (Cusabio, Houston, TX, USA) and 1:500 diluted rabbit polyclonal anti-HuC/D (Abcam, Cambridge, United Kingdom) overnight at 4°C· followed by incubation with Cy3-labeled goat anti-mouse IgG (Beyotime ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: 5-FU and wdr12 samples were immunolabeled with anti-HuC/D antibodies (not shown) (ab210554, abcam, used at a dilution of 1:600). Anti-GFP (GFP-1020 ...