pVectOZ CAT Transfection control citations for abcam

Quote: ... The remaining lysates were equally divided and incubated with 7 μl IgG (negative control, CST) and FTO antibody (Abcam), respectively ...

Whole Genome and Embryo Transcriptome Analysis of Vertebrate Identifies nxhl Controlling Angiogenesis by Targeting VE-PTP

Honglin Luo, et al., bioRxiv - Genomics 2020

Quote: ... Magnetic beads were pretreated with an anti-rabbit IgG (Beyotime, China; negative control) or anti-Nucleolin (ab50279, Abcam, UK) for 1 h at room temperature ...

Endogenous Syngap1 Alpha Splice Forms Promote Cognitive Function and Seizure Protection

Murat Kilinc, et al., bioRxiv - Neuroscience 2021

Quote: ... Lysates were then incubated for 2hrs at RT with 1.5mg Dynabeads (10004D, Thermo) functionalized with 10ug of anti-PSD95 (Thermo, MA1-045) or IgG control (ab18415, Abcam). After extensive washing ...

A Novel CRISPR-Engineered, Stem Cell-Derived Cellular Vaccine

Krishnendu Chakraborty, et al., bioRxiv - Immunology 2021

Quote: ... Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam) according to kit protocol and analyzing the dilution of the dye at 24-hour periods over 3-days and measuring fluorescence intensity ...

miR-100-5p downregulates mTOR to suppress the proliferation, migration and invasion of prostate cancer cells

Su-Liang Li, Yun Ye, Jian-Jun Wang, bioRxiv - Cancer Biology 2020

Quote: ... which was used as a loading control and horseradish peroxidase (HRP)-coupled goat anti-rabbit IgG H&L (1:5,000; ab97051; Abcam) as a secondary antibody ...

EMC is required for biogenesis and membrane insertion of Xport-A, an essential chaperone of rhodopsin-1 and the TRP channel

Catarina J. Gaspar, et al., bioRxiv - Cell Biology 2021

Quote: ... Control staining of actin in the rhabdomeres of adult retina was performed with Phalloidin-FITC (1:500) (Abcam 235137). After rinsing three times with PBT ...

Evolutionary variation in MADS-box dimerization affects floral development and protein degradation dynamics in maize

Maria Jazmin Abraham-Juarez, et al., bioRxiv - Plant Biology 2020

Quote: ... Membrane was stripped and tubulin was detected as a loading control by incubation with 1:25,000 Mouse monoclonal anti-TUB DM1A (Abcam). Detection was done using 1:10,000 Anti-Mouse secondary HRP-conjugated antibody (Amersham ECL GE Cat ...

Erythroid precursors and progenitors suppress adaptive immunity and get invaded by SARS-CoV-2

Shima Shahbaz, et al., bioRxiv - Immunology 2020

Quote: ... HT29 (human colorectal adenocarcinoma grade II) whole cell lysate was used as a positive control for TMPRSS2 (Abcam, ab3952). Full length predicated at 54 kDa and the cleavage fragment at 25 ...

High through-put identification of miR-145 targets in human articular chondrocytes

Aida Martinez-Sanchez, et al., bioRxiv - Cell Biology 2020

Quote: ... male) transfected with miR-145 or control mimics for 24h using 90 μl Sepharose G beads (Abcam, Cambridge, UK) pre-coated with 6ug of mouse anti-human Ago2 (clone2E12-1C9 ...

Splicing of the SynGAP Carboxyl-Terminus Enables Isoform-Specific Tuning of NMDA Receptor Signaling Linked to Cognitive Function

Murat Kilinc, et al., bioRxiv - Neuroscience 2020

Quote: ... Lysates were then incubated for 2hrs at RT with 1.5mg Dynabeads (10004D, Thermo) functionalized with 10ug of anti-PSD95 (Thermo, MA1-045) or IgG control (ab18415, Abcam). After extensive washing ...

Eos promotes T_H2 differentiation by propagating the IL-2/STAT5 signaling pathway

Jasmine A. Tuazon, et al., bioRxiv - Immunology 2022

Quote: ... Resulting chromatin fragments were immunoprecipitated with antibodies against STAT5 (R&D AF2168, 10 μg/IP) or IgG control (Abcam ab6709 ...

Heritable changes in chromatin contacts linked to transgenerational obesity

Richard C. Chang, et al., bioRxiv - Developmental Biology 2022

Quote: ... complexed to 2.5 μg antibody (anti-CTCF, ab128873, anti-RAD21, ab217678, or Isotype IgG control, ab171870, Abcam, Cambridge, UK). Beads were washed three times with LiCl buffer (50 mM HEPES-KOH ...

Amyloid-β exposed astrocytes induce iron transport from endothelial cells at the blood-brain barrier by altering the ratio of apo- and holo-transferrin

Stephanie L. Baringer, et al., bioRxiv - Neuroscience 2023

Quote: ... cells were co-incubated with either 50 nM Aβ or untreated control and 1:50 CCK8 reagent (Abcam, ab228554) and astroglial media ...

FGFR1 governs iron metabolism via regulating post-translational modification of IRP2 in prostate cancer cells

Hui Lin, et al., bioRxiv - Cancer Biology 2022

Quote: ... anti-FBXL5 (Cat#ab140175) and anti-FGFR1 (Cat#ab824) were obtained from Abcam. The membranes were washed with TBS with Tween 20 (TBST ...

Genome folding principles revealed in condensin-depleted mitotic chromosomes

Han Zhao, et al., bioRxiv - Genomics 2023

Quote: ... HP1β(CST Cat#8676, 5μl/IP) and HP1γ (Abcam Cat#ab217999, 5μl/IP) respectively ...

PTPN1/2 inhibition induces highly functional terminal effector CD8 T cells through autocrine IL-10

Luis-Alberto Perez-Quintero, et al., bioRxiv - Immunology 2023

Quote: ... Antibodies against STAT2 (Cat#ab134192) and pSTAT2 Y690 (Cat#ab53132) were from Abcam. The antibody for ShcA was from BD Transduction Laboratories (Cat#S14603) ...

Loss of Tsc1 from striatal direct pathway neurons impairs endocannabinoid-LTD and enhances motor routine learning

Katelyn N. Benthall, et al., bioRxiv - Neuroscience 2020

Quote: ... For intrinsic excitability experiments, NBQX (10 μM, Fisher, cat. #104410), CPP (10 μM, Fisher, cat. #24710) and picrotoxin (50 μM, Abcam, cat. #120315) were added to the external solution to block synaptic transmission ...

Activation of pancreatic β-cell genes by multiplex epigenetic CRISPR-editing

Gimenez Carla Alejandra, et al., bioRxiv - Molecular Biology 2020

Quote: ... Protein expression at day 10 post transfection was analyzed by immunocytochemistry using the following antibodies and dilutions: PDX1 1:100 (134150, Abcam), NEUROG3 1:200 (HPA039785) ...

Natural Occurring Non-Synonymous Single Nucleotide Polymorphisms in Integrase and RNase H Regulate Assembly and Autoprocessing of HIV-1

Tomozumi Imamichi, et al., bioRxiv - Microbiology 2021

Quote: ... anti-HIV IN antibody (Cat#: ab66645) and anti-HIV p55+p24+p17 antibody (Cat #: ab63917) and anti-Tsg101 (Cat#: 30871) were obtained from Abcam (Cambridge, MA, USA), anti-Alix antibody (Cat# ...

Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

N Kurgan, et al., bioRxiv - Physiology 2022

Quote: ... Total oxphos antibody cocktail (cat#ab110413) and UCP1 (cat#ab10983) and serine 406 phospho-ATGL (cat# ab135093) antibodies were purchased from Abcam (Cambridge, United Kingdom). PGC-1α (cat#AB3242 ...

ATF2 phosphorylation is essential for neuronal apoptosis, linking the DLK/LZK kinase cascade to JUN upregulation

Jorge Gomez-Deza, et al., bioRxiv - Neuroscience 2023

Quote: ... Cells were washed three times with PBS and DAPI was used as a nuclear counterstain. Antibodies used: p-cJun (S63) (CST, cat. no. 9261S), total cJun (CST, cat. no. 9165) p-ATF2 (T71) (Abcam, cat. no. ab32019) 488-conjugated cJun (SCBT ...

ATF2 phosphorylation is essential for neuronal apoptosis, linking the DLK/LZK kinase cascade to JUN upregulation

Jorge Gomez-Deza, et al., bioRxiv - Neuroscience 2023

Quote: ... Sections were washed three times with PBS and DAPI was used as a nuclear counterstain. Antibodies used: p-cJun (S63) (CST, cat. no. 9261S), total Jun (CST, cat. no. 9165) p-ATF2 (T71) (Abcam, cat. no. ab32019).

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