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Citations for abcam :
301 - 350 of 2151 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection was done with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam) and mouse anti ssDNA (clone16-19 ...
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bioRxiv - Bioengineering 2020Quote: ... Glycogen levels were determined using a standard assay according to manufacturer’s instructions for fluorometric detection (Abcam, #ab65620). Glucose quantification assay ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were detected by secondary antibody VeriblotBlot for IP detection reagent (HRP) (ab131366, abcam, 1:3000 dilution), ECL rabbit IgG HRP-linked F(ab’)2 fragment ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked in milk prior to detection with specific antibodies: 1:1000 ACE2 rabbit (Abcam, Ab108209), 1:1000 TMPRSS2 rabbit (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... a rabbit anti-caspase-3 for detection of cleaved forms of caspase-3 (Abcam #9664, 1:1000), mouse anti-GAPDH Mab (Proteintech #60004-1-Ig ...
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bioRxiv - Bioengineering 2023Quote: ... biotinylated detection antibody was added (biotinylation of carrier and preservation-free antibody was performed with the Abcam Lightning Link biotinylation kit ...
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bioRxiv - Physiology 2024Quote: ... The ROS activities were quantitated with DCFDA/H2DCFDA – Cellular Reactive Oxygen Species Detection Assay (Abcam cat# ab113851). Both assays were used according to protocol recommended by manufacturer.
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bioRxiv - Neuroscience 2020Quote: ... Sections were allowed to cool down to RT and tissue was delineated with pap-pen (Abcam, # ab2601). Free floating sections were left at RT for 2 hr prior 3 × 15 min washes with PBS to remove cryopreservation solution ...
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bioRxiv - Bioengineering 2022Quote: ... Then the organoids were incubated for 2 hours at RT with primary antibodies against cytokeratin 8 (Abcam, Cambridge ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 h at RT with primary antibody specific for spike (Abcam, cat# ab252690) or N-protein (Sino Biological ...
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bioRxiv - Genetics 2023Quote: ... and secondary antibody incubation was performed for 2 hours at RT (goat anti mouse A647 (Abcam, 150115), goat anti rabbit A555 (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with the following primary antibodies for 1 h at RT: Anti-HA (Abcam-Ab18181), Anti-RhCMV-Rh152/151 (mouse mAb generated in house by OHSU monoclonal antibody shared resource (42)) ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS for 30 min at room temperature (RT) and incubated with anti-BrdU/CldU (Abcam, ab6326) diluted in blocking buffer for 1 h at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... and re-probed for the detection of the respective total protein (anti-SMAD3 [ab40854]; anti-SMAD5 [ab40771]: Abcam) and loading control (anti-β-Actin [A1978] ...
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection of BrdU was with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam). Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution ...
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bioRxiv - Cancer Biology 2020Quote: The concentration of ATP was determined 72h after siRNA transfection using the luminescent ATP detection system (Abcam, ab113849) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... (Cat# ab131368, RRID:AB_2895114) and VeriBlot for IP Detection Reagent (HRP) (Cat# ab131366, RRID:AB_2892718) were from Abcam (Cambridge, UK). Antibody against β-tubulin (Cat# sc-5274 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies with respective dilutions were used for target detection: 1:10 000 anti-SFPQ (Abcam), 1:5000 anti-FUS (ProteinTech) ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was performed using mainly goat anti-rabbit IgG H&L (DyLight® 488) (1:500, Abcam ab96883), Cy3-donkey anti guinea pig IgG H+L (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG as detection antibody were purchased from Abcam (Cambridge, UK). Capture antigens PDIA6 (ab101048) ...
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bioRxiv - Microbiology 2024Quote: Exponentially growing Leptospira (2×109) were used to determine ATP concentration using the luminescent ATP detection assay (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... 0.03% Triton X100 in PBS for 1h at RT and incubated with chicken anti-GFP (AbCam, ab 13970) or goat anti-Sox10 (Santa Cruz ...
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bioRxiv - Genomics 2022Quote: The following primary antibodies were used for RT&Tag and CUT&Tag experiments: rabbit anti-IgG (Abcam ab172730), rabbit anti-MSL2 (gift from Mitzi Kuroda ...
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bioRxiv - Microbiology 2022Quote: ... 927-40000) for 30 minutes at RT and then probed with primary antibodies against ACE2 (1:3000, Abcam, ab15348 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubation was done in blocking buffer for 1 h at rt (anti PR Ser294Phospho, ab61785, Abcam). Primary antibody was washed 3 times in wash buffer (0.2% BSA ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times and 100 μl of TMB ELISA Substrate (Abcam) were added to each well ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times and 100 μl of TMB ELISA Substrate (Abcam) were added to each well ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed five times and added with TMB substrate solution (Abcam) followed by 15 minutes of incubation ...
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bioRxiv - Neuroscience 2020Quote: ... 7um sections of adipose tissues was performed for detection of UCP1 (RRID:AB_2241462, 1:500, Abcam, Cambridge, UK. Cat. #10983). Alexa 488 (2.5µg.mL ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with the fluorescent labelling reagent Fluo-4 AM (at 1μM in D-MEM) for the detection of intracellular calcium (GR3332801, Abcam) for 1 hour at 35°C ...
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection for BrdU was done with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam), together with mouse anti ssDNA (clone16-19 ...
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bioRxiv - Immunology 2020Quote: ... cells were activated as above for and then fixed and permeabilized followed by detection with pPyk2 Y402 (Abcam, ab4800) and secondary detection with goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in milk or BSA prior to detection with specific antibodies: 1:1000 ACE2 rabbit (Abcam, Ab108209), 1:5000 GAPDH rabbit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... after which a Novolink Polymer detection system44 was used with an Abcam anti-NF-κB antibody (Abcam, Cambridge, UK) at a 1 in 1500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... and specific band detection was achieved by the use of rabbit monoclonal anti-4E-BP1 (clone Y329, 1:2,000, ab32024, Abcam) and rabbit polyclonal anti-p-4E- BP1 antibody (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% dry milk power in PBS for 1 hour at RT and incubated overnight at 4°C in primary antibody (anti-Lamin B1 1µg/ml, Abcam ab16048 ...
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bioRxiv - Cell Biology 2021Quote: ... The CldU and IdU tracts were labelled (for 2.5 hr in the dark, at RT) with anti-BrdU antibodies recognizing CldU (rat; Abcam) and IdU (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilized in 0,02% Triton X-100 at RT for 15 minutes and incubated with anti-HA antibody (1:1000, #9110, Abcam) in staining buffer at RT for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1h at RT with goat anti-mouse IgG conjugated with Alexa Fluor-555 (Abcam, UK) (1:500 dilution in blocking buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies used for western blotting was anti-cltc (1:1000 in 0.1% Tween20, 0.05% milk in PBS, RT 1h, Abcam ab21679) and anti-GAPDH (1:10000 in TBST ...
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bioRxiv - Cell Biology 2022Quote: ... diluted in blocking buffer for one hour at RT: donkey anti-rat Alexa Fluor® 647 (1:250; abcam); donkey anti-goat Alexa Fluor® 568 (1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The newly replicated CldU and IdU tracks were stained for 2.5 hours at RT using two different anti-BrdU antibodies recognizing CldU (#ab6326, Abcam) and IdU (#347580 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... blots were incubated for 1 h at RT with Rat HRP-conjugated α-mouse (1:10,000, Abcam mAb 131368). To analyze the co-immunoprecipitations ...
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bioRxiv - Microbiology 2023Quote: ... samples were washed and incubated for 1 hr at RT with Anti-HA Rb Ab (1:500, Abcam, ab9110) in blocking buffer ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Duke University] and GAPDH (mouse anti-human GAPDH, 1:10,000, 1 hr at RT; Cat: ab8245, Abcam, Waltham, MA). Primary antibodies were detected using donkey anti-rabbit IRDye® 800CW IgG (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody mouse anti-Nup153 (Abcam) at 1:250 for 30 m ...