Labshake search

301 - 350 of 1351 citations for Recombinant Human ROBO4 His tagged since 2019

• Molecular and functional alterations in the cerebral microvasculature in an optimized mouse model of sepsis-associated cognitive dysfunction

  Paulo Ávila-Gómez, et al., bioRxiv - Neuroscience 2024

  Quote: ... Recombinant Alexa Fluor 647 Anti-P2Y12 antibody ([EPR26298-93] ab308432, Abcam), anti-Fibrinogen (Rabbit Polyclonal ab34269 ...
• Ultrasonic cerebrospinal fluid clearance improves outcomes in hemorrhagic brain injury models

  Matine M. Azadian, et al., bioRxiv - Bioengineering 2024

  Quote: ... recombinant rabbit anti-IBA-1 (1:500; Abcam, Cat No. ab178846), and chicken anti-GFAP antibody (1:500 ...
• Multiplexed identification of RAS paralog imbalance as a driver of lung cancer growth

  Rui Tang, et al., bioRxiv - Cancer Biology 2021

  Quote: ... human mitochondria (Abcam, ab92824), pERK (Cell Signaling ...
• A multi-layered systems approach for renal cell carcinoma

  Lindsay S. Cooley, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Human CFB (abcam, ab137973).
• High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

  Ok-Hee Kim, et al., bioRxiv - Physiology 2019

  Quote: ... human intact PTH (Abcam), soluble RANKL (Immundiagnostik ...
• Fibrinogen and Complement Factor H are promising CSF protein biomarker(s) for Parkinson’s disease with cognitive impairment- A Proteomics and ELISA based study

  Aditi Naskar, et al., bioRxiv - Neuroscience 2021

  Quote: ... (Human Fibrinogen: ab208036 Abcam UK ...
• Towards establishing Extracellular Vesicle-associated RNAs as biomarkers for HER2+ breast cancer

  Colin L. Hisey, et al., bioRxiv - Cancer Biology 2020

  Quote: ... human EpCAM (AbCAM ab223582), and human TSG101 (AbCAM ab30871 ...
• SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis

  Adam L. Bailey, et al., bioRxiv - Immunology 2020

  Quote: ... human CCR2 (Abcam ab176390), SARS-CoV-2-N (Sino Biological 40143-R001 ...
• SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis

  Adam L. Bailey, et al., bioRxiv - Immunology 2020

  Quote: ... human ACE2 (Abcam ab15348), human Ki67 (Abcam ab16667) ...
• SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis

  Adam L. Bailey, et al., bioRxiv - Immunology 2020

  Quote: ... human Ki67 (Abcam ab16667), human CCR2 (Abcam ab176390) ...
• Targeting oncogenic Wnt/β-catenin signaling in adrenocortical carcinoma disrupts ECM expression and impairs tumor growth

  Morgan K. Penny, et al., bioRxiv - Cancer Biology 2022

  Quote: ... or Human Nucleoli (Abcam ab190710 ...
• Photoswitchable binders enable temporal dissection of endogenous protein function

  Michael Westberg, et al., bioRxiv - Biochemistry 2023

  Quote: ... or human EGF (Abcam).
• YAP dysregulation triggers hypertrophy by CCN2 secretion and TGFβ uptake in human pluripotent stem cell-derived cardiomyocytes

  Orlando Chirikian, et al., bioRxiv - Cell Biology 2024

  Quote: ... Human CCN2 (Abcam-261851) and TGFβ1 (Abcam-100647 ...
• An Ultrasensitive Biosensor for Quantifying the Interaction of SARS-CoV-2 and Its Receptor ACE2 in Cells and in vitro

  Xiaolong Yang, et al., bioRxiv - Biochemistry 2020

  Quote: ... The blot was first probed with anti-6×His mouse monoclonal antibody (Abcam ab18184), followed by incubation with goat anti-mouse IgG secondary antibody (Jackson ImmunoResearch) ...
• Neural Stem Cells Secreting Bispecific T Cell Engager to Induce Selective Anti-Glioma Activity

  Katarzyna C. Pituch, et al., bioRxiv - Immunology 2020

  Quote: ... Bound BiTE proteins were detected with HRP-conjugated anti-HIS antibodies (Abcam, Cambridge, MA) using 1-Step™ Slow TMB-ELISA (Thermo Scientific ...
• Neuronal pentraxin Nptx2 regulates complement activity in the brain

  Jiechao Zhou, et al., bioRxiv - Neuroscience 2022

  Quote: ... The readout was HRP-conjugated anti-His tag antibody recognizing the pentraxin proteins (abcam).
• Oct1 cooperates with Smad transcription factors to promote mesodermal lineage specification

  Jelena Perovanovic, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Hi-ChIP was performed as previously described (55) using H3K27Ac antibodies (Abcam, ab4729, RRID:AB_2118291) and two biological replicates for each condition ...
• Statistical mining of triple-negative breast cancer-specific nanobodies among huge libraries from immunized alpacas

  Ryota Maeda, et al., bioRxiv - Cancer Biology 2023

  Quote: The antibodies used for immunohistochemistry (IHC) were anti-His (rabbit monoclonal EPR20547: ab213204, Abcam), anti-Her2 (mouse monoclonal anti-erbB-2 ...
• Pioneer transcription factors are associated with the modulation of DNA methylation patterns across cancers

  Roza Berhanu Lemma, et al., bioRxiv - Cancer Biology 2021

  Quote: ... N-terminally FLAG- and HA-tagged TET1 from the mammalian expression plasmid pEF1-FH-TET1 (ABCAM) was digested with BmgBI (blunt end cutter ...
• The molecular organization of flat and curved caveolae indicates bendable structural units at the plasma membrane

  Claudia Matthaeus, et al., bioRxiv - Cell Biology 2022

  Quote: ... Caveolin1 was tagged with specific antibody (anti-Caveolin1-Rabbit, abcam, 1:100 in 3% BSA/PBS), and a secondary Rabbit antibody labelled with 12 nm gold particles (Dianova ...
• Unique Cancer Motility Behaviors in Confined Spaces of Microgroove Topography with Acute Wall Angles

  T. Yaginuma, K. Kushiro, M. Takai, bioRxiv - Cancer Biology 2019

  Quote: ... was incubated overnight then tagged with secondary Alexa Fluor 488 goat anti-mouse IgG antibody (Abcam) for 2 hr to visualize the vinculin molecules ...
• Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation

  Annabelle Dold, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Expression of tagged Mkrn1 was verified by western blot analysis and immunostaining using anti-GFP (Abcam) or anti-FLAG (Sigma).
• Arginine methylation sites on SepIVA help balance elongation and septation of the cell wall in Mycobacterium smegmatis

  Angela H Freeman, et al., bioRxiv - Microbiology 2022

  Quote: ... Strep-tagged proteins were detected using rabbit anti-Strep-tag II antibody (1:1000, Abcam, ab76949) in Tris-buffered saline with Tween 20 (TBST ...
• UFMylation regulates translational homeostasis and cell cycle progression

  Igor A. Gak, et al., bioRxiv - Cell Biology 2020

  Quote: ... Rabbit monoclonal recombinant Anti-UFM1 antibody [EPR4264(2)] (Abcam, ab109305, 1:1000), Rabbit polyclonal UFL1 Antibody (Bethyl Laboratories ...
• UFMylation regulates translational homeostasis and cell cycle progression

  Igor A. Gak, et al., bioRxiv - Cell Biology 2020

  Quote: Rabbit monoclonal recombinant Anti-UFC1 antibody [EPR15014-102] (Abcam, ab189252, 1:1000), Rabbit monoclonal recombinant Anti-UBA5 antibody [EPR11729] (Abcam ...
• Eliminating fibroblast activation protein-α expressing cells by photoimmunotheranostics

  Jiefu Jin, et al., bioRxiv - Cancer Biology 2021

  Quote: ... A recombinant anti-FAP-α monoclonal rabbit antibody ab207178 (clone EPR20021, abcam) was used to probe human/murine FAP-α ...
• Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20

  Mohamed Rasheed Gadalla, et al., bioRxiv - Microbiology 2019

  Quote: ... either recombinant anti-rabbit IgG VHH Single Domain (1:5000, abcam, ab191866) or goat anti-mouse IgG (H + L) ...
• Mutant SF3B1 promotes PDAC malignancy through TGF-β resistance

  Patrik T. Simmler, et al., bioRxiv - Cancer Biology 2022

  Quote: ... The following antibodies were used for immunoblotting: Recombinant anti-GADPH (EPR16891, Abcam) and rabbit monoclonal anti-MAP3K7 (anti-TAK1 ...
• SOX9 acts as a dynamic pioneer factor inducing stable changes in the chromatin landscape to reprogram endothelial cells

  Bettina M. Fuglerud, et al., bioRxiv - Genomics 2021

  Quote: ... The array was incubated with 2 µg recombinant SOX9 protein (ab131911, Abcam) at 4 °C overnight in CHAPS immunoprecipitation buffer (Fivephoton Biochemicals) ...
• Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer’s Disease Mice

  Baruh Polis, et al., bioRxiv - Neuroscience 2019

  Quote: The recombinant monoclonal antibody diluted at 1:20000 #ab183830 (Abcam, Cambridge, UK) was utilized for the detection of MAP2 protein ...
• Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform

  Denisa Bojkova, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... and TMPRSS2 (1:1000 dilution, Recombinant Anti-TMPRSS2 antibody [EPR3861], #ab92323, Abcam) followed by incubation with IRDye-labeled secondary antibodies (LI-COR Biotechnology ...
• mTORC3 activity is regulated by GSK3β through the activation of PKCδ that phosphorylates and activates STAT1

  Jun Zhan, et al., bioRxiv - Cell Biology 2024

  Quote: ... Purified recombinant STAT1 protein was used as a substrate (Abcam, Cambridge, UK). Prior to performing the kinase assay ...
• Armored Bicistronic CAR T Cells with Dominant-negative TGF-β Receptor II to Overcome Resistance in Glioblastoma

  Nannan Li, et al., bioRxiv - Cancer Biology 2024

  Quote: Immunohistochemical staining used a recombinant anti-TGFβ1 antibody (ab215715, Abcam, Waltham, Boston) and DAPI on tissue sections derived from NSG mice ...
• Temporal Coordination of the Transcription Factor Response to H₂O₂ stress

  Elizabeth Jose, et al., bioRxiv - Cell Biology 2023

  Quote: ... Recombinant Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) (1:1000) from Abcam and actin (Sigma ...
• An Alzheimer’s disease risk variant in TTC3 modifies the actin cytoskeleton organization and the PI3K-Akt signaling pathway in iPSC-derived forebrain neurons

  Holly N. Cukier, et al., bioRxiv - Genetics 2023

  Quote: ... the primary antibodies were added [Recombinant Anti-PAX6 antibody [EPR15858] (Abcam, # ab195045), Human/Mouse/Rat SOX1 Antibody (R&D Systems ...
• Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq

  Sara Sunshine, et al., bioRxiv - Systems Biology 2023

  Quote: ... Cells were stimulated with recombinant TNF-α (50 ng/ml; Abcam ab9642) and imaged using confocal microscopy 25 and 45 minutes after stimulation ...
• Lineage plasticity enables low-ER luminal tumors to evolve and gain basal-like traits

  Gadisti Aisha Mohamed, et al., bioRxiv - Cancer Biology 2022

  Quote: ... before staining with Recombinant anti-Cytokeratin 8 antibody Alexa Fluor 647 (Abcam, ab192468 ...
• PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)

  Krishan Kumar Saini, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Recombinant Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (cat# ab125066) obtained from Abcam. Anti-ATF6α antibody clone# (37-1 ...
• Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during aging

  Ye Xiao, et al., bioRxiv - Cell Biology 2022

  Quote: ... total cell lysates were collected and incubated with antibody (ab9108; anti-His tag antibody; Abcam) overnight at 4°C ...
• Recombinant expression and characterisation of a lipase from the Antarctic zooplankton Salpa thompsoni

  Ekta Rayani, et al., bioRxiv - Synthetic Biology 2022

  Quote: ... The membrane was incubated with the primary antibody: Anti-His mouse monoclonal antibody (ab18184, Abcam) diluted 1:1000 in TBST-M for two hours ...
• Base-edited CAR T Cells for combinational therapy against T cell malignancies

  Christos Georgiadis, et al., bioRxiv - Molecular Biology 2020

  Quote: ... followed by Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (catalogue ab117504, Abcam). Cells were acquired on a Cyan or on a BD LSRII (BD Biosciences) ...
• Extracellular vesicles induce aggressive lung cancer via non-canonical integrin-EGFR-KRAS signaling

  Karla Rubio, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Western blotting was performed using standard methods and antibodies specific for 6x-HIS-Tag (Abcam), GFP (Santa Cruz) ...
• Whether the ADP-ribosyltransferase activity of Ta-sro1, a noncanonical PARP protein, contributes to its function in salinity-stress tolerance?

  Shu-Wei Liu, et al., bioRxiv - Molecular Biology 2022

  Quote: ... All of forms of Ta-sro1 were detected with anti-His antibody (Abcam, Cambridge, UK).
• DNA binding activity of CAMTA3 is essential for its function: Identification of critical amino acids for its transcriptional activity

  Kasavajhala V.S.K. Prasad, et al., bioRxiv - Plant Biology 2023

  Quote: ... The membrane was then probed with an anti 6X His Tag monoclonal antibody (Abcam; ab49746) conjugated to alkaline phosphatase at 1:2000 dilution and detected with an alkaline phosphatase detection system.
• Intercalation of the disulfide bond between the A2 and A4 loop of Cellobiohydrolase (Cel7A) of Aspergillus fumigatus enhances catalytic activity and thermostability

  Subba Reddy Dodda, et al., bioRxiv - Biochemistry 2023

  Quote: ... Western blot further confirmed the purified protein with an anti-His monoclonal antibody (Abcam, USA).
• Acquired RAD51C promoter methylation loss causes PARP inhibitor resistance in high grade serous ovarian carcinoma

  Ksenija Nesic, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Rabbit anti-human RAD51 (ab133534) and mouse anti-human Geminin antibodies (ab104306) from Abcam, Human-specific EpCam-647 (clone VU1D9 ...
• Alpha-toxin elicited CX₃CL1-release via ADAM10 in Staphylococcus aureus pneumonia impairs bactericidal function of human monocytes

  Srikanth Mairpady Shambat, et al., bioRxiv - Microbiology 2023

  Quote: ... Immunohistochemistry staining was used to detect human CD14 and human CX3CL1 (Abcam; MM0207-8J23) on paraffin-embedded biopsies ...
• Streptococcus pyogenes pharyngitis elicits diverse antibody responses to key vaccine antigens influenced by the imprint of past infections

  Joshua Osowicki, et al., bioRxiv - Immunology 2024

  Quote: ... Human salivary IgA was quantified using the Human IgA SimpleStep ELISA® Kit (Abcam) according to the manufacturer’s instructions ...
• Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses

  Jared Feldman, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human IgG-HRP (Abcam), was added to each well at 1:20,000 dilution in PBS-T and incubated for 1hr at RT ...
• Structure of a germline-like human antibody defines a neutralizing epitope on the SARS-CoV-2 spike NTD

  Clara G. Altomare, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human IgG-Biotin (Abcam) in PBS-T was added to each and incubated for 1hr at RT ...