Labshake search
Citations for abcam :
301 - 350 of 435 citations for RGS4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: Immunostaining for p62 and ubiquitin was performed with blocking and permeabilization buffer (1% saponin, 3% bovine serum albumin) and anti-p62 rabbit monoclonal antibodies (Abcam #AB109012) at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2 ...
-
bioRxiv - Neuroscience 2019Quote: ... 1% goat serum with Triton was used as a blocking solution for at least 1 hour at RT and a Goat Anti-Rat IgG H&L (HRP) (Abcam ab97057) was used as a secondary and incubated for 1.5 hours at RT before continuing with the described DAB staining ...
-
bioRxiv - Physiology 2019Quote: ... samples were incubated overnight at 4°C with the following antibodies diluted in 3% BSA blocking solution and 1% horse serum: Anti-cTnT (Abcam, ab33589), anti-cTni (Abcam ...
-
bioRxiv - Genetics 2020Quote: ... and then incubated in blocking solution overnight at 4°C with the following antibodies: rabbit monoclonal anti-ATP6V1A (1:1000)(Abcam EPR19270), rabbit polyclonal anti-GNE (1:1000)(Proteintech 25079-1-AP) ...
-
bioRxiv - Immunology 2021Quote: ... All steps with tissue sectioning and IHC are the same as with mouse tissue through blocking in 10% normal donkey serum (Abcam, ab7475) for 20 minutes at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... for 30 min at room temperature and then incubated in blocking solution containing goat anti-GFP (to recognize BFP, 1:1000, Abcam, ab6673) and rabbit anti-RFP (to recognize mCherry ...
-
bioRxiv - Molecular Biology 2022Quote: ... mitochondria were immunostained in 100 μL of Intercept Blocking Buffer containing 1 μL of Alexa Fluor 647 Anti-TOM20 antibody (Abcam, ab209606) for 1 h at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a primary antibody blocking solution including both rabbit anti-PV (1:500; Abcam Cat. #ab11427, Pittsburgh, PA), and WFA lectin fluorescein (1:100 ...
-
bioRxiv - Developmental Biology 2022Quote: ... for 1 hr at RT then stained O/N at 4° C using the following primary antibodies diluted in blocking buffer: Chromogranin A (1:200, Abcam, ab15160), Glucagon (1:200 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.7% Triton X-100 in 1X PBS) for 2 hours at room temperature and incubated in blocking solution with anti-GFP antibody (1:500, Abcam, ab6658) overnight at 4 ºC ...
-
bioRxiv - Microbiology 2020Quote: ... the section was blocked with blocking buffer (Zymed Laboratories Inc., San Diego, USA) before incubated with anti-Citrobacter antibody (Abcam, ab37056) overnight at 4°C as previously described[33] ...
-
bioRxiv - Neuroscience 2020Quote: ... 115-007-003) was used for antigen blocking before overnight incubation at 4 °C with rhodopsin 4D2 (1:500, Abcam, ab98887). Slides were washed and incubated with goat anti-mouse 488 (1:1000 ...
-
bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked for 1 hr at RT in 3% BSA PBS 0.1% Tween-20 (PBS-T) and then incubated overnight at 4°C with primary antibody (rabbit anti-RGS9, 1:500 in blocking solution; Abcam, UK). After washing three times in PBS-T ...
-
bioRxiv - Developmental Biology 2022Quote: ... blocked for 1 h in blocking buffer (PBST with 10% donkey serum) and incubated with the primary antibody chicken anti-GFP (1:250 in blocking buffer) (Abcam, ab13970) overnight at 4 ºC ...
-
bioRxiv - Cell Biology 2022Quote: ... and incubated with the indicated primary antibodies diluted in blocking buffer at 4°C overnight: mouse-pH3 (1:2000, Abcam, ab14955), rabbit-Ki67 (1:500 ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in Licor blocking buffer and probed with the following primary antibodies for histone H3 (1:1000; Abcam #ab1791), H3K9me2 (1:1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
-
bioRxiv - Cell Biology 2019Quote: ... Same experiment was also performed blocking monocyte-derived macrophages at RT for 15 min with 10 μg/ml of mAb α-Siglec-1 7–239 (Abcam), IgG1 isotype control (BD Biosciences ...
-
bioRxiv - Microbiology 2019Quote: ... After blocking, the cells were stained with Mouse Anti-Human Myeloperoxidase (Santacruz, Germany) and Rabbit Anti-Human Histone-H3 (Abcam, USA) antibodies for one hour ...
-
bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... western blotting (WB) and WB blocking conditions were as follows: mouse anti-β-actin (Abcam, 8226, WB 1:2000 in BSA), mouse anti-ZEB2 (Origene ...
-
bioRxiv - Genomics 2021Quote: ... The cells were resuspended by gently flicking the tube in the same blocking buffer containing 1:250 dilution of rabbit anti lamin-B1 antibody (Abcam, #ab16048) and incubated overnight at 4 °C in a microfuge tube rack ...
-
bioRxiv - Physiology 2020Quote: ... Membranes were incubated overnight at 4 °C with the indicated primary antibodies diluted in blocking solution: NT3 (1:1000, Abcam ab65804), TrkC (1:750 ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were then treated with the blocking solution (10% Goat serum) for 1 h at RT and incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher ...
-
bioRxiv - Bioengineering 2021Quote: ... Primary antibody solutions were prepared by diluting one or more of the following antibodies in blocking buffer: mouse anti-HNF4a (1/200 from stock, Abcam ab41898), goat anti-OPN (1/60 from stock ...
-
bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
-
bioRxiv - Immunology 2021Quote: ... Coverslips were incubated overnight at 4 °C in blocking buffer and probed the following morning with 1 :500 rabbit anti-ACE2 (Abcam ab15348) for 1h at room temperature on an orbital rocker ...
-
bioRxiv - Developmental Biology 2020Quote: ... Then the membranes were incubated with following primary antibodies at appropriate dilution in blocking buffer: ant-SATB1 (ab109122, Abcam 1: 10000), anti-SATB2 (ab92446 ...
-
bioRxiv - Developmental Biology 2022Quote: ... β1-integrin blocking antibodies at 100 μg/mL (MilliporeSigma; # MABT821) or mouse monoclonal (P5D2) β1-integrin blocking antibodies at 50 μg/mL (Abcam; ab24693).
-
bioRxiv - Physiology 2022Quote: ... slides were incubated overnight at 4°C in primary antibodies (1:500 dilution in blocking solution) for glucagon (Abcam, cat. # ab10988) and insulin (Cat ...
-
bioRxiv - Neuroscience 2022Quote: ... Free floating sections were washed three times in PBS (10 minutes each) and then incubated in a blocking solution containing 10% normal goat serum (NGS, Abcam #ab7481), 0.5% Triton X-100 in PBS for 2 hours at room temperature on a shaker ...
-
bioRxiv - Neuroscience 2022Quote: ... for 1 hour at room temperature prior to overnight incubation on a shaker in primary antibody solution (1:2000 Rabbit α GFP diluted in blocking solution, Abcam, #ab6556 ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with the primary antibody (chicken anti-GFP 1:800 in blocking solution; ab13970, Abcam, Cambridge, UK) overnight at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
-
bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C in a blocking-permeabilizing solution containing rabbit anti-SOX10 (1:100, Abcam, ab227680). The next day ...
-
bioRxiv - Biophysics 2023Quote: ... cells were incubated in a blocking buffer with 20 ng/μl of commercial secondary antibody goat anti-mouse IgG H&L (Alexa Fluor 647) (abcam, ab150115) for 45 min ...
-
bioRxiv - Genetics 2023Quote: ... Slides were then washed with PBS-T once more before blocking for 1 hour at room temperature with 10% goat serum (abcam #ab7481) or 1% Bovine Serum Albumin (Sigma #05482 ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated in 2x PBS with 0.2% Tween and blocking buffer (1:1) with primary antibodies rabbit anti-AUF1 (Abcam, 1:5000), rabbit anti-phospho-AUF1 (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution (1:500 Rabbit anti-GFP Abcam 290; 1:50 Mouse anti-HP1a DSHB, C1A9) and incubated with embryos at 4°C overnight ...
-
bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing anti-goat IgG H&L HRP-conjugated secondary antibody (1:1000) (Abcam, ab6885) for 1hr ...
-
bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...
-
bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were left to incubate in primary antibody diluted in blocking solution for 30-45 min (chicken anti-GFP (Abcam ab13970) 1:500 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): anti-CD146 (Abcam #ab75769); anti-#-SMA (Abcam #ab5694 ...
-
bioRxiv - Genetics 2023Quote: ... Blocking solution was removed and replaced with blocking solution containing primary antibodies against dsRNA (Scicons, Szirák, Hungary) and alpha tubulin (Abcam, Cambridge, UK) (both diluted 1:1000) ...