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Citations for abcam :
3401 - 3450 of 4975 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation of the cleared lysates was performed overnight at 4C with 5μg of anti-VDR antibody or anti-RXRα monoclonal antibodies (Abcam – Table 2). The beaded complexes were washed several times with NP-40 lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 1% normal goat serum (NGS) for 1 hour and incubated overnight with primary recombinant anti-prolactin receptor antibody [EPR7184(2)] (abcam, ab170935) at 1:50 in 1% BSA ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubations were performed overnight at 4°C in PBS-T with 2% NGS plus one or more of the following antibodies: polyclonal rabbit anti-GFP (1:800, Abcam #ab290), monoclonal mouse anti-Abd-B (1:500 ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with the SARS-CoV-2 Spike Antibody Mouse Mab (40592-MM117, SinoBiological, China) and HRP-conjugated goat anti-mouse IgG (ab6789, abcam, UK). Detection was carried out using an Ultra High Sensitivity ECL Kit (HY-K1005 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocked cells were then incubated for 1 hour at room temperature with primary antibodies anti-Isl1/2 (Abcam ab109517, 1:2000), anti-Chx10 (Santa Cruz Biotechnology sc-365519 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was conducted using 2% w/v BSA in 1×PBS for 1 h and incubated with anti-Ki67 antibody (Abcam ab16667) in a 1:200 dilution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was then incubated in blocking buffer (5% skim milk in TBS/0.05% tween-20) followed by blotting with primary antibodies (anti-SARS-CoV-2 spike (Abcam ab275759, USA) 1:2,000 or anti-β-actin (Sigma A2228 ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked with 5% normal goat serum/2% BSA/0.1% Triton-X for 1 hour and incubated with mouse monoclonal PAX7 antibody (abcam, 1:200) and rabbit monoclonal SOX10 antibody (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated in 1%BSA and then overnight at 4 degrees in primary antibody (anti-GAD65/67, Abcam ab11070, 1:500 (Cheng et al., 2017)) ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: chicken polyclonal anti-mouse GFAP (Abcam ref. ab4674, RRID AB_304558, 1:3000), rabbit polyclonal anti-mouse IBA1 (Wako ref ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin extract was sonicated with Bioruptor (20 min; pulse: 30 s on/30 s off; high amplitude) and immunoprecipitated with H3K27ac antibody (Abcam, ab4729, rabbit polyclonal, 4 µg/ChIP) premixed with Dynabeads A and G ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and then incubated for approximately 20 hours at 4°C in either rabbit anti-α-synuclein (α-syn) phosphorylated serine 129 (P-S129; Abcam #EP1536Y, Cambridge, United Kingdom) diluted to 1:1000 in blocking solution or rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Molecular Biology 2020Quote: ... After blocking in 5% milk/TBST membranes were probed with primary anti-ACE2 antibody (Abcam 15348 for total ACE2 or Abcam 108252. for long ACE2) followed by the appropriate secondary HRP-conjugated antibody (Dako) ...
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bioRxiv - Genetics 2020Quote: ... chromatins from freshly isolated or cultured SCs were fragmented using sonicator and incubated with 5 μg antibody against histone H3-K27 acetylation (Abcam, ab4729, rabbit poly-clonal) overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... embryos and cells were blocked at room temperature in 5% BSA in PBS for 1 hour and incubated with primary antibodies (hCGβ, Abcam, ab9582; TBX3, Abcam, ab99302) in blocking solution overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... in 1X TBS-T with 5% (w/v) BSA and then with anti-rabbit HRP-conjugated secondary antibody (Abcam, Cat # ab205718; diluted 1:7,500). For signal visualization ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:1000 dilution) at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 ng/mL RIPK1 primary detection antibody (1:300 dilution in Meso Scale Discovery Diluent 100 of Abcam ab125072 lot GR3258748, 50 µL/well) was incubated for 2 h at RT with shaking at 600 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed three times in PBS containing 0.01% Triton X-100 and incubated for 2 hours with AlexaFluor conjugated donkey anti-rabbit secondary antibody (Abcam ab150073 1:1000). After secondary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the same ACE2 IHC protocol was applied to other 2 primary antibodies anti-Human ACE2: monoclonal rabbit anti-Human ACE2 (cat. Ab108252, Abcam, Cambridge, UK) diluted 1:100 in PBS 1× supplemented with 5% goat serum ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Genomics 2020Quote: ... Blocking with 1% BSA/PBS for 1h was followed by incubation with the following primary antibodies for 1-2 h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Microbiology 2020Quote: ... Each IP comprised of 100 μl of lysate and 2 μg of an antigen-specific antibody against p50 (ab7971, Abcam, Cambridge, UK), p65 (ab7970 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots (2 µg per lane) were probed with primary antibodies specific for CD68 (ab213363, 1/500 in blocking buffer; Abcam, Cambridge, UK) or β-actin (4967S ...
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bioRxiv - Cell Biology 2021Quote: ... lysates of transduced cortical neurons cultured in 6-well plates were were incubated overnight with 2 µl of rabbit α-AP-3µ1 antibody (Abcam, Reference ab201227) on a rotating wheel at 4°C ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... The cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min at 37 °C with or without dye-labeled phalloidin (ab176753 or ab176759, Abcam, Cambridge, UK) for 1 h at 37 °C ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... Nuclei from one striatum (~10,000) were washed 2X and then resuspended in Digitonin Buffer with either anti-H3K4me3 (Antibody 1: Abcam, Ab8580 (Figure 2); Antibody 2 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in PBS plus 2% NBCS and 0.3% Triton X-100: mouse anti-Glutamate decarboxylase (65 kD isoform, GAD65; 1:1500; ab26113; Abcam, Cambridge, United Kingdom), rabbit anti-Calbindin (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Genomics 2022Quote: ... were washed twice with PBS/BSA and incubated with either 0.5 μl of anti-TBP antibody (gift from Dr. Joseph Reese, Pennsylvania State University) overnight or with 2 μl of anti-H3 (Abcam, cat # ab1791) for 3 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were blocked with 5 % (v/v) FBS in PBS for 30 min and incubated with a rabbit polyclonal anti-COX-2 primary antibody (ab15191, Abcam, Cambridge, UK) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was removed using a magnetic Eppendorf tube rack prior to adding 2 µg of anti-citrulline antibody (ACAb, Abcam, Cambridge, UK) diluted in 200 µL phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...