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Citations for abcam :
3351 - 3400 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415; YTHDF1 antibody, Abcam ab220162) and incubated at room temperature for one hour ...
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bioRxiv - Microbiology 2022Quote: Immunohistochemistry was performed on formalin-fixed cryosections of mouse aortic roots using antibodies to identify macrophages (MOMA-2, 1:50; ab33451, Abcam, Cambridge, MA), followed by detection with biotinylated secondary antibodies (1:400 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated in the solution of primary antibodies: anti-glial fibrillar acidic protein (GFAP) rabbit polyclonal antibody (Abcam, Cambridge, UK, catalog number ab7260) and anti-neuronal nuclear protein (NeuN ...
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bioRxiv - Microbiology 2023Quote: ... rabbit antibodies recognizing cellular proteins (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-VPS35, Abcam, ab157220, 1:200). PLA was performed with Duolink reagents (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and a rabbit antibody recognizing a cellular marker protein (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-VPS35, Abcam, ab157220, 1:200). PLA was performed with Duolink reagents (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: PNPT1 and SUV3L1 proteins were detected using anti-PNPT1 antibodies and anti-SUV3L1 antibodies at 1:1000 (both obtained from Abcam, Cat #: ab176845 and Cat #: ab157109). pPKR ...
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies were applied for 1-2 hours at room temperature and are as follows: Goat α-Rabbit-HRP (Abcam, ab97051, 1:5000), Donkey α-Rabbit-HRP (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin (125 µg/IP) was incubated overnight in 2 µg of either rabbit polyclonal anti-histone 3 lysine 4 trimethyl (H3K4me3; Abcam, Cat# ab8580, RRID:AB_306649) or anti-Histone 3 (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed into a LoBind tube carefully so as not to remove any beads and 2 µg of anti-GFP antibody (Abcam ab290, anti-rabbit) were added to the sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2-4 μg/ml in blocking buffer (PBS + 4% FBS) containing 1 μg/ml mouse anti-tubulin (Abcam, ab7291, Cambridge, UK). After washing with PBS ...
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bioRxiv - Physiology 2019Quote: Cyst sections were blocked and permeabilized with 3% normal donkey serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam, Cambridge, United Kingdom) in 3% normal donkey serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Cell Biology 2021Quote: ... ∼ 5×1010 EVs collected from the two steps of purification were diluted into 500 μl of PBS and 2 μg of rabbit monoclonal anti-CD9 (Abcam, Cambridge, U.K., ab92726) or rabbit IgG (Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Physiology 2021Quote: ... 4µm sections of paraffin-embedded mouse kidneys were submitted to antigen retrieval for 20 min at 95°C in citrate buffer followed by incubation with Ly-6B.2 antibody (Abcam, ab53457, 1:100). Sections were incubated with HRP-labeled secondary antibody (Vector ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Media was changed 2 h post-infection and the cells were fixed and immunostained 48 h post-infection to detect firefly luciferase (Abcam #ab181640, RRID # AB_2889835).
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxy-6-pentadecyl-benzamide (CTPB) (CAS #: 586976-24-1) (>99% purity) was purchased from Abcam (Cambridge, United Kingdom). For both chemicals ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Genomics 2022Quote: ... The cells were washed three times with PBS and resuspended with 2% BSA-PBS containing a goat anti-rabbit IgG FITC conjugated secondary antibody (Abcam catalog no. ab6717) and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Neuroscience 2022Quote: ... slices were pre-incubated at room temperature for 2 hours in a blocking medium composed of PB-Tx and Normal Goat Serum (NGS, 5% or 10%, Abcam, Cat. No. ab7481), after which they were incubated with primary antibodies (72 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Cancer Biology 2020Quote: ... Testosterone level was measured using the Human Testosterone ELISA Kit from Abcam (ab174569). In order to recapitulate the levels of supraphysiological testosterone administrated in clinical trials35 ...
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bioRxiv - Cell Biology 2020Quote: MTP was determined using a JC-1 mitochondrial membrane potential assay kit (Abcam). Oxidative phosphorylation and glycolysis were determined using a Seahorse XFe24 extracellular flux analyzer (Seahorse Bioscience ...
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bioRxiv - Genetics 2021Quote: Nuclear extract was prepared from about 108 cells using Chromatin Extraction kit (Abcam). Extracts were immunoprecipitated with rabbit anti-HA (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: IGF2 secreted peptide was assayed with the Mouse IGF2 ELISA KIT (Abcam, ab100696) on 10 independent samples.
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bioRxiv - Developmental Biology 2020Quote: ... immunostaining was modified to follow protocol for Mouse on Mouse Immunodetection kit (Abcam). For BrdU ...