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Citations for abcam :
3251 - 3300 of 10000+ citations for Goat Anti Human IgG FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... A rat IgG isotype primary (Abcam, #ab27478) was used to confirm the specificity of the secondary antibody binding to anti-human CD8 ...
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bioRxiv - Developmental Biology 2023Quote: ... Normal rabbit IgG (Abcam, ab172730, 1:100), PE-conjugated mouse IgM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... or rabbit α-mouse IgG (abcam #ab46540) overnight at 4°C with nutation ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry/RFP (rabbit IgG, 1:500, Abcam), mCherry/RFP (chicken IgY ...
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bioRxiv - Immunology 2023Quote: ... or isotype control (Rabbit IgG, Abcam #EPR25A) at 1.58 μg/ml for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... or rabbit IgG isotype control (Abcam, ab172730) in PBS containing 0.02% Tween 20 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell solution was then mixed and incubated with Annexin-V-FITC and PI (Abcam) working solution for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... medium was changed and fixed/stained 72 h later with CD44-FITC conjugated antibody (Abcam), CD24 antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: Apoptotic cells were detected using the FITC Annexin V Apoptosis Detection Kit (cat# ab14085, Abcam). EBV positive and negative cells were infected with lentivirus shCtrl or shplod1 ...
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bioRxiv - Developmental Biology 2024Quote: The apoptosis assay was performed using the Annexin V-FITC Apoptosis Detection Kit (Abcam, #ab14085), while the proliferation assay was conducted employing the BrdU incorporation method (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... tissue sections were incubated for 1 hour at room temperature with donkey anti-rabbit IgG polyclonal antibody conjugated with horseradish peroxidase (Cat. No. ab6802, abcam, RRID:AB_955445) diluted to 1:300 in blocking serum ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed three times with PBS-T and then incubated with secondary HRP-conjugated anti-rabbit IgG (Abcam, Cambridge, UK) or anti-mouse IgG (Invitrogen™ ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibodies (FLI 7.3 mouse, Santa Cruz; H3K27ac rabbit, Abcam ab4729; Rabbit anti-mouse IgG, Abcam ab46540; LSD1 rabbit, Abcam ab17721) were added at a dilution of 1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... and Griffonia simplicifolia isolectin conjugated with Rhodamine (labels endothelial cells/blood vessels) followed by incubation with a second antibody (Alexa Fluor 647 Donkey Anti-Rabbit IgG, Abcam ab150075) and BODIPY to stain lipid ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were washed 3 times for 10 minutes at room temperature with TBST and incubated with either anti-Mouse IgG-HRP (1:20 000, Abcam, ab6728) or anti-Rabbit IgG-HRP (1:20 000 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with one of the following secondary antibodies: Donkey Anti-Rat IgG H&L (#150154, Alexa Fluor 555, abcam, UK) (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... each sample was primary stained using 1:200 dilution of rabbit unconjugated polyclonal anti-CD3 IgG (CAT # ab5690; Abcam, Cambridge, MA), or an equivalent concentration of rabbit unconjugated anti-mouse polyclonal IgG as an isotype control (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... one for an equivalent amount of normal rabbit IgG control and the second incubated with ChIP-grade anti-MeCP2 (Abcam, ab2828) at 4°C with agitation overnight ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... The membranes were then washed 3 × 5 min each with TBST and incubated with anti-rabbit IgG – HRP secondary antibody (abcam 6721) at 1:3000 in 5% BSA – TBST for 2 h at room temp ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: Concentrations of anti-CMV IgG relative to a standard curve and internal negative and positive control were determined by ELISA (Abcam, UK) and read in a microplate spectrophotometer (Bio-Rad xMark ...
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bioRxiv - Microbiology 2020Quote: ... Anti-mouse IgG conjugated to HRP for immunoblot of immunoprecipitation samples (used in Fig. 3b) was purchased from Abcam (Cat#: ab131368). Fluorophore-conjugated secondaries for immunofluorescence were purchased from Life Technologies.
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were pelleted and washed with PBS twice and then co-incubated in detection antibody (donkey anti rabbit IgG-AF488: Abcam[Ab150093])) diluted 1:5000 in PBS and incubated for 15 minutes at 4 degrees ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times in PBS for 15 min and incubated in secondary antibody for 2 h at room temperature: anti-mouse IgG (1/1,000, HRP-conjugated, Abcam, No. ab205719), anti-guinea pig IgG (H + L ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 hours at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (dilution 1:5000, Abcam #ab6789). After washing again ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Myotubes were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS-T and 100 uL anti-hamster IgG horseradish peroxidase antibody (HRP, abcam, #ab6892) diluted 1:10,000 in PBS-T containing 1% milk powder was added to all wells ...
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bioRxiv - Plant Biology 2023Quote: ... diluted to 1:125 was used as primary antibody and Donkey Anti-Mouse IgG H&L Alexa Fluor® 555(Abcam) diluted to 1:500 was used as secondary ...
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bioRxiv - Plant Biology 2024Quote: ... blots were stripped and re-probed with anti-HSC70 monoclonal antibody (Enzo #ADI-SPA-818-D) at 1/5000 dilution followed by Rabbit Anti-Mouse IgG H&L (HRP) secondary antibody (AbCam ab6728) at 1/10000 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... and human complement C3 levels were measured using the Human Complement C3 ELISA Kit (ab108823, Abcam) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Bioengineering 2019Quote: ... both rabbit anti-human (Rockland 600-401-104, 1:200, for collagen II, Abcam ab138492, 1:1500 for collagen I) were applied in 1 % NGS ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Cell Biology 2021Quote: ... bound IRE1α LD-Fc was transferred after washes to a PVDF membrane and detected with anti-human Fc antibody (ab977225, Abcam), ECL solution (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated for 24 hours with the following antibodies (1:1000 v/v dilution in TBST): i) mouse anti-human OxPhos cocktail (Abcam; Cambridge ...
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bioRxiv - Immunology 2022Quote: ... For the analysis of cell surface molecules cells were stained with APC anti-human CD48 (MEM-102; Abcam, Cambridge, UK); APC-anti human 2B4/CD244 (2-69 ...
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bioRxiv - Bioengineering 2022Quote: ... Antibodies were as follows: i) for human cells: mouse IgG1 anti-CD44/CD73/CD90/CD45/CD105/CD14/HLA-DR (all from Abcam); ii ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercial polyclonal and monoclonal antisera were used (m, mouse; h, human and r, rat proteins): anti-LIMPII (ab165222) was from Abcam; anti-Calnexin (2679) ...
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bioRxiv - Cancer Biology 2020Quote: ... The stainings were performed using either CDK6 monoclonal rabbit anti-human antibody (Clone EPR4515, dilution 1:250, Abcam, Cambridge, UK) or Cyclin D2 monoclonal rabbit anti-human antibody (Clone D52F9 ...
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bioRxiv - Cell Biology 2019Quote: ... RUNX-2 positive cells were similarly stained using a mouse anti-human RUNX-2 monoclonal antibody (ab76956, Abcam, Cambridge, UK), which was revealed using an Alexa Fluor®488 goat anti-mouse IgG2a secondary antibody (A-21131 ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Bioengineering 2022Quote: ... for detection (brown signals) of collagen type I (1:100 anti-human collagen I alpha-1 1200-1450 fragment (ab233080, Abcam) compatible to pig elastin [45] ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-human MT1-MMP rabbit monoclonal antibody EP1264Y against the catalytic domain (Abcam, catalog no. ab51074, 1:1000 dilution) were used followed by anti-rabbit secondary antibodies (Li-COR ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were probed with the following primary antibodies: rabbit polyclonal anti-calreticulin (1:1000) (ab19261, abcam; to detect human CRT); chicken polyclonal anti-calreticulin (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...