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Citations for abcam :
3201 - 3250 of 3329 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were gently washed in phosphate buffered saline (PBS) prior to blocking with the blocking reagent from the mouse-on-mouse polymer IHC Kit (Abcam ab269452). The tissues were then incubated overnight with the primary antibody at 4°C ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Immunohistochemistry for polymerised α1-antitrypsin was performed using 2C1 monoclonal primary antibody and a Mouse-on-Mouse Polymer kit (Abcam AB127055) for detection ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear protein sample extract from washed cell pellets was carried out according to the manufactures protocol using cell fraction kit – standard (Abcam, ab109719). Nuclear pellets were resuspended in 100mM ammonium bicarbonate and lysed by sonication at 26 joules ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with CM for 24 or 48h after which cells were stained to detect DNA fragmentation in apoptosis using the BrdU-Red DNA (TUNEL) kit (Abcam, ab66110) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: A luminescent ATP detection assay kit was used to determine the total levels of cellular ATP as per manufacturer’s instructions (abcam, Cat #ab113849). Briefly ...
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bioRxiv - Biochemistry 2021Quote: Histological analyses of the NAFLD tissues were performed using the H&E Stain Kit (Hematoxylin and Eosin) (ab245880; Abcam, Cambridge, UK) and the Oil Red O Stain Kit (Lipid Stain ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Olympus BX60F microscope with an SC50 camera.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by trypsinisation and Annexin V staining was carried out using an Annexin V-iFluor 647 Apoptosis Detection Kit (Abcam, ab219919), following manufac-turer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Sorted satellite cells were treated with UV radiation at different dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal α-GPEET and α-EP IgG were conjugated with Allophycocyanin (APC) fluorescence molecule by APC Conjugation Kit – Lightning Link (Abcam). Cells were stained with α-GPEET or α-EP or both α-GPEET and α-EP to detect the loss of GPEET and EP as the indicator of the presence of non-PCF cells in the induced culture ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...
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bioRxiv - Genetics 2022Quote: ... The mixture was centrifuged at 10000g for 5 min and 5μl of the supernatant was used to measure fumarate levels per instruction from the commercial fumarate kit (Abcam, Cambridge, UK).
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bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in Acetic Acid solution (Picrosirius Red Stain Kit, Abcam, ab150681) and imaging ...
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bioRxiv - Systems Biology 2024Quote: ... The detection of EdU incorporation was performed according to the protocol provided by the EdU proliferation kit (Abcam, ab219801, iFluor 488). In brief ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential (ΔΨm) was measured with the JC-1 mitochondrial membrane potential assay kit (ab113580, Abcam, Cambridge, United Kingdom) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was vacuum dried and the dried lipids were dissolved in 200 μL of the assay buffer supplied by the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, ab65359). 5 μL of each sample was used for each reaction.
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... ROS indicator dye was prepared according to the manufacturer’s protocol using DCFDA/H2DCFDA - Cellular ROS Assay Kit (Abcam, ab113851, Cambridge, UK), and was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... Cresyl Violet & Luxol Blue staining of brain sections was performed using a commercially available kit according to the protocol provided (Abcam, ab150675). Brain images were taken on a Zeiss Axio Scan Z1 slide scanner.
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bioRxiv - Immunology 2024Quote: ... was performed by flow cytometry and triglyceride release assay was determined in supernatant after low-speed centrifugation (300 g, 10 min) using Triglyceride Assay kit (Abcam ab65336). GMPs were collected in a supernatant of granulocytes (3-5 x 106/ml ...
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bioRxiv - Genetics 2023Quote: ... slides were stained for goblet cells and mucins using an Alcian Blue dye and Nuclear Red Fast counterstain kit (Abcam; ab150662). Quantification of the percent area of Alcian blue staining ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted 1:400 in Milli-Q water and lactate concentration was measured using a Lactate assay kit (Abcam, ab65330) according to the manufacturer’s protocol (using 1.5 µL of Enzyme Mix per well) ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC labeling for c-Myc was performed using the DISCOVERY HQ+ Amp kit with primary antibody against c-MYC (1:600, Abcam, ab32072). IHC for EdU and MYC on cells grown on chamber slides was performed by following the protocol described before (48) ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation was accomplished using plate based-beads in the EpiQuik Kit along with HNF4α antibodies (Novus NB100-1783 and Abcam ab181604) to validate enrichment at QPRT ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Cell Biology 2023Quote: Senescence-associated β-galactosidase (SA-β-gal) was used to detect MSCs senescence using SA-β-gal staining kit (Abcam, AB65351) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were fixed and stained to detect β-galactosidase activity using the Senescence Detection Kit from Abcam (ab65351, Abcam, www.abcam.com).
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... These different recombinants were used to measure phospholipase A2 (PLA2) activity following procedures described in the kit manual (Abcam, Cambridge, UK), in the presence of ethanol or P4 (10-5M) ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...