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Citations for abcam :
2951 - 3000 of 10000+ citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Primary antibodies were as follows (species, isotype, manufacturer and dilution are given): lamin A (mouse monoclonal IgG3, Abcam, 1:200); nesprin-1 (rabbit monoclonal IgG ...
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bioRxiv - Genomics 2019Quote: ... Coverslips were washed three times for 5 minutes each with PBT and incubated with diluted secondary antibody (anti-mouse labelled with Alexa Fluor 488, 1:800, ab150113, Abcam; anti-rabbit labelled with Alexa Fluor 647 ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature and then incubated overnight at 4°C with primary antibody: mouse anti-GAPDH (1:5000, Abcam) or rabbit anti-parvalbumin (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies where used for western-blotting at 1/1000 dilution: anti-Rpb1-CTD clone 8wG16 (mouse, monoclonal, Abcam), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Bioengineering 2020Quote: ... Blots were blocked with 3% BSA in TBST (Tris Buffered Saline with Tween 20) for 1 h and then incubated with mouse anti-tau (Abcam) and rabbit anti-beta actin (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then blocked for 1 hour with a 5% milk-TBS solution and incubated overnight at room temperature with a 1:1,00 dilution of mouse monoclonal antibody against TPP1 (Abcam ab54685) in 5% milk-TBS ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated for 30 min with Alexa488-conjugated goat anti mouse and Alexa546 conjugated donkey anti rabbit secondary antibodies (1/400e, AbCam). After washing ...
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bioRxiv - Cell Biology 2019Quote: ... (1:250 for IF) and Alexa Fluor 647-conjugated goat against mouse IgM (1:500 for FACS) (# ab150123) were from Abcam. Mouse mAbs against UBE2J1 (# sc-377002 ...
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bioRxiv - Genetics 2019Quote: ... The cells were fixed in 4% paraformaldehyde and permeabilized with saponin and lysosomes were labeled using 10 μg/mL dilution of FITC-labeled antibody against mouse Lamp-1 (ab24871, abcam), a lysosomal structural protein ...
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bioRxiv - Immunology 2020Quote: ... serum was diluted 1:5 in diluent buffer and run in duplicate using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), CENP-E (mouse ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SMO (Santa Cruz sc-166685, mouse IgG2a in IF 1:200; Abcam ab236465 for Figs. 5B, D and E), anti-PTCH1 (Abcam ab53715 ...
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bioRxiv - Pathology 2021Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-mouse MMP-13 (1/6000; ab39012; Abcam) and MMP-1 (1/500 ...
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bioRxiv - Cell Biology 2020Quote: ... blocked with Odyssey Blocking Buffer (Li-Cor 927-40000) and incubated with CENP-A Mouse antibody (1:500; Ab13939; Abcam) in 5% BSA TBS with 0.1% Tween 20 and detected with IRDye 800CW and 680LT secondary antibodies using the Odyssey Infrared Imaging System (Li-Cor).
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bioRxiv - Cell Biology 2020Quote: ... TZM-bl cells were similarly transfected followed by staining with 1:50 dilution of anti-mouse monoclonal CD4-APC conjugated antibody (Abcam) or isotype control (Abcam ...
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bioRxiv - Genetics 2021Quote: ... Then localization of c-Fos was monitored using Alexa Fluor 594 (Goat Anti-Mouse, 1:500, Abcam, ab150116, Cambridge, USA) and Alexa Fluor 488 (Goat Anti-Rabbit ...
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bioRxiv - Microbiology 2021Quote: ... Then the cells were incubated with goat anti-mouse IgG Alexa Fluor 488 secondary antibody or goat anti-rabbit IgG TRITC secondary antibody (1:1000 dilution; Abcam), respectively ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Microbiology 2022Quote: ... then were washed and was incubated with 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). The non–permeabilized samples stained for accessible E-cadherin were permeabilized with PBS-0.2% Triton 100-X for 10 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with fluorophore-conjugated secondary antibodies at 1:1,000 dilutions (Alexa Fluor 568 goat anti-mouse, Abcam, ab175473; Alexa Fluor 488 goat anti-rabbit, Abcam, ab150077) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... anti ICAM1 (4915, Cell Signalling Technologies), anti-YAP1 (4912, Cell Signalling Techologies) or 1:5000 dilution of mouse anti-GAPDH (ab8245, Abcam) antibody ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed 3x with PBST and incubated 1 hr at 22°C in 100 µL anti-mouse IgG AlexaFluor 594 secondary antibody (ab150116, Abcam) diluted 1:200 in PBST + 1% bovine serum albumin + 10% goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then incubated with secondary antibodies (diluted 1:2500 in nonfat dry milk): goat anti-rabbit IgG HRP or goat anti-mouse IgG HRP (all from Abcam). Bands were visualised using Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... the cells were then incubated with the following primary antibodies in 0.1% BSA solution (1X PBS) overnight in the dark at 4 °C: mouse monoclonal (1:1000) Respiratory Syncytial Virus (F-protein) (Abcam), rabbit monoclonal (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245 ...
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bioRxiv - Immunology 2024Quote: ... Immunocytokines were diluted to relevant concentrations in PBSTA and detected via an HRP-conjugated anti-mouse IgG secondary diluted 1:3000 (Abcam). 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... we also included a subsequent incubation with the secondary rabbit anti-mouse IgG antibody (used at a 1:100 dilution; Abcam, cat ...
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bioRxiv - Plant Biology 2022Quote: ... The membrane was washed twice with PBST for 10 min each before the addition of the secondary anti-mouse Ig-HRP antibody (1: 15,000, Abcam, ab6708) or anti-rabbit Ig-HRP antibody (1:15,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in 1:250 dilution of anti-ERK3 antibody (mouse Cat# MAB3196, R&D or rabbit Cat# ab53277, Abcam) in blocking solution for 1 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were stained with sheep anti-human CD31 (R&D) at a 1:40 dilution and mouse anti-human alpha smooth muscle actin (Abcam) at a 1:200 dilution for 2 h at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... at 1:10,000 followed by incubation with either secondary goat pAb to mouse IgG HRP (ab97040, Abcam, Lot # GR3219575-4) at 1:10,000 or secondary mouse antibody to rabbit IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... spanning the entire M1 area in each mouse were stained with antibodies against NeuN (1:1000, catalog number: ab177487, Abcam) followed by Alexa Fluor 568 goat anti-rabbit (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Then blocked tissues were then incubated with specific primary antibodies against anti-CD31 antibody [P2B1] ab24590 (1:500, mouse monoclonal, ab72234, Abcam) and anti-TRIM47 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in blocking buffer (2x 10 min) and incubated with secondary antibodies: Alexa Fluor 488 goat anti-mouse (1:1000, Abcam), Alexa Fluor 594 goat anti-rabbit (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... Lysates were sonicated to generate DNA fragments with sizes of 0.5 - 1 kb and immunoprecipitated with mouse monoclonal anti-H3K9me2 (abcam, ab1220), rabbit polyclonal anti-H3K9me3 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... combined with the secondary antibody of anti-Mouse polyclonal goat antibody conjugated with Alexa Fluor 647 (1:1000, ab150115, Abcam) to label PV+ neurons in the IC ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used in this work: goat anti-mouse IgG coupled with FITC (1:1000, Abcam, ab6785).
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bioRxiv - Evolutionary Biology 2023Quote: ... the brains were washed for 3 x 10 minutes in PBST and incubated with 1:200 goat anti-mouse IgG (ab150115, abcam) in 5 % NGS for 48 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... washed 3 times with PBS and incubated with anti-mouse secondary antibodies conjugated with Alexa647 (ab150107, Abcam, 1:1000 dilution) or Alexa488 (A21202 ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107 ...
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bioRxiv - Microbiology 2023Quote: ... Stained cells were than washed with PBS twice and incubated with 1:500 AF488 fluorochrome-conjugated goat anti-mouse IgG H&L (Abcam) in the staining buffer for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with Goat HRP-coupled secondary anti-mouse IgG (Abcam, cat no: ab205719, 1:10K dilution) or anti-rabbit IgG (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an Alexa Fluor®647 conjugated donkey anti-mouse polyclonal antibody at a 1:1000 concentration (ab150111; Abcam). Antibodies were validated in-house as described below ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an Alexa Fluor®647 conjugated donkey anti-mouse polyclonal antibody at a 1:1000 concentration (ab150111; Abcam). dsRNA was visualized using a mouse monoclonal anti-dsRNA antibody at a 1:500 concentration (MABE1134-25UL ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an Alexa Fluor®647 conjugated donkey anti-mouse polyclonal antibody at a 1:1000 concentration (ab150111; Abcam). hACE2 was visualized using a mouse monoclonal anti-ACE2 antibody at a 1:500 concentration (sc-390851 ...