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Citations for abcam :
251 - 300 of 3495 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were stained overnight at 4°C with the following primary antibodies: anti-ORC1 (1:1000 TBST/5% milk; Abcam, ab85830). Immunoblots were then incubated in secondary goat anti-rabbit (1:1000 TBST/5% milk ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated for 48 hours at 4 °C with primary antibodies diluted in 5% NGS in PBS-T as follows: rabbit anti-cFos (1:1000, Abcam, #190289) and chicken anti-GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations: α-smooth muscle actin (αSMA, mouse, Cat. No. ab7817, Abcam) at 1:300 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in TBST containing 5% BSA: DYRK1A (1:1000; Abcam, ab65220), FAM53C (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... blocked spheroids were firstly incubated for 2 days at 4 °C with α-Ki67 (Abcam, stock concentration 0.031 mg/mL) or α-cleaved caspase3 (Cell Signalling Technology ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pCS2-lg-HAtag-WT and pCS2-lg-HAtag-MUT (2 μg) (Supplementary Fig. 4) were then transfected into the HEK293T cell line (ab255449, Abcam) separately ...
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bioRxiv - Genetics 2020Quote: ... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with TBST containing 5% skim milk or 5% BSA for 2 hours and incubated overnight at 4°C with the following antibodies: anti-MIF (1:200, ab65869, Abcam), anti-iNOS (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... All slides then were incubated at 4°C overnight with a single IF stain of ACE-2 (1:100, ab15348, Abcam) or ACE-2 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sheared chromatin was diluted to contain 0.16% SDS for antibody binding and incubated overnight at 4 °C with 2 ug anti-H3K27Ac (Abcam, ab4729), anti-H3K4ME3 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed using the confor-mation-specific α-synuclein antibody MJF-14 (MJFR-14-6-4-2; Abcam, #ab214033) or the total α-synu-clein antibody syn211 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rotated for 2 hours at 4 °C in 200 μL D-Wash with 1:200 LMNB2 (Abcam, ab8983) followed by a wash step with D-Wash ...
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bioRxiv - Neuroscience 2024Quote: Dot blots were used to confirm immunoreactivity of generated PFFs with a commonly used conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam). 1.5 µl of serially diluted monomer or (un)sonicated PFF samples were loaded onto nitrocellulose membrane (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was quantified by dot blot (described above) using a conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam), and diluted PFFs as a standard series ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hrs at room temperature before incubation with primary antibody (1:500, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in blocking solution overnight at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mg of protein lysate was incubated overnight at 4°C with 2 µg of MHC-I mouse antibody (ab70328, Abcam) or mouse IgG1 isotype control antibody (5415 ...
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bioRxiv - Physiology 2020Quote: ... rinsed in PBS 2×10min and then incubated in blocking solution containing 5% normal goat serum (NGS, Ab7481, Abcam), 0.5% Triton (T8787 ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was measured after 3 days and 5 days of culture using an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (Abcam, ab211091) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Cell proliferation was measured using a 5-bromo-2-deoxyuridine (BrdU) labeling and a proliferation ELISA Kit (Abcam, ab126556) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 4-Hydroxynonenal (4-HNE, 1:200; Abcam, # ab48506), Alexa Fluor 555 donkey anti-rabbit (1:300 ...
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bioRxiv - Physiology 2024Quote: ... The fasting free fatty acid level was assessed using a Free Fatty Acid Assay Kit (ab65341, Abcam, United Kingdom) following the manufacturer’s instructions and measured using a SpectraMax ABS microplate reader.
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bioRxiv - Neuroscience 2020Quote: ... The fixed cells were blocked with 5% normal goat serum for 4 hours followed by incubation with anti-Iba-1 antibody (1:300, Abcam, Cambridge, UK) for 12 hours at 4°C to label microglial cells ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 2 hours at room temperature and incubated overnight at 4°C using the following concentrations: anti-H3K9me3 (1:300, Abcam ab8898), anti-H3K27me3 (1:300 ...
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bioRxiv - Biochemistry 2024Quote: ... and then incubated overnight at 4°C with primary antibodies: Anti-beta Amyloid 1-40 [EPR23712-2] (AbCam, Cat. No. ab289991) for Aβ40 and Anti-Amyloid Beta [21F12] (Absolute Antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...