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Citations for abcam :
251 - 300 of 1486 citations for Ethyl 6 2 morpholinomethyl phenyl 6 oxohexanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded in 6-well plates for 24h prior to 30 minutes incubation with 10µM EdU (Abcam, #ab146186). Cells were then harvested ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... The IgG amount in that reference sample was interpolated as an average across exposures from a concentration curve calculated in GraphPad Prism 6 from a blot of known concentrations (IgG from Abcam ab133470 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were washed 3x 30min in PBT and stained for at least 6 hours in secondary antibodies 1:500 in PBT at room temperature (Goat anti-Rabbit Alexa 488 Abcam cat#150077 ...
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bioRxiv - Synthetic Biology 2021Quote: ... for Im7 variants or 50 μL of 1:5,000-diluted HRP-conjugated anti-6×His tag antibody (Abcam, catalog # ab1187) for scFv-HER2 variants ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated for 6 hours on a rotating wheel at 4°C with primary antibodies MTHFD2 (Abcam; #ab151447; 5 μg) or negative control IgG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The equivalent of 3-6 x107 cells were precipitated overnight with 1 ug IgG/106 cells of rabbit antibody against H3K27ac (ab4729, Abcam). Before antibody precipitation ...
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bioRxiv - Neuroscience 2024Quote: ... coli), monoclonal Xarr1-6 anti-SAG (Peterson et al., 2003) and monoclonal G8 anti-GRK1 (Zhao et al., 1998)(Abcam). Images were obtained using a Zeiss 510 meta confocal microscope equipped with a 40X N.A ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... an sIPSC baseline was recorded for 6 minutes and compared to sIPSCs after 20 minutes wash in of 1 μM TTX (Abcam), 0.2 μM K252a (Tocris) ...
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bioRxiv - Immunology 2022Quote: ... Each well of a 6-well plate was incubated for 30min at room temperature with 200μL of Cell Extraction Buffer PTR (ab195215, ABCam, UK) containing phosphatase inhibitors and the protease inhibitor aprotinin ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBST and stained for 1 hour with primary antibodies (ZEB1: Bethyl, IHC-00419; E-Cadherin: BD Biosciences, 610181; Collagen 6: Abcam, ab182744 ...
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bioRxiv - Physiology 2024Quote: We utilized two commercially available enzyme-linked immunosorbent assay (ELISA) kits for the quantitative analysis of IL-6 (Abcam, ab100713) and IL-8 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-mouse IgG H&L (6 nm Gold) (CAT NO. ab39614) and anti-TSG101 antibody (CAT NO. ab30871) were purchased from Abcam. Alexa Fluor 568 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... tissue sections were incubated in boiling citrate buffer with 0.05% Tween 20 at 95°C (pH 6) for 20 minutes or treated with Pepsin C (Cat. No. ab64201; Abcam) for 10 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... If not otherwise mentioned heat induced antigen retrieval with citrate buffer (pH 6) was performed prior to incubation with the following rabbit mono or poly-clonal antibodies: UCP1 (Abcam ab10983) 1:800 ...
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bioRxiv - Cell Biology 2022Quote: Epidermal punch biopsies (6 mm diameter) in triplicates were floated on medium containing either a mouse α-RTN1A ab (mon162, abcam) or the respective isotype control ab (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... slides were performed on the automaton Leica Bond RX and unmasked at pH 6 before incubated 30 minutes with an anti-CD8 antibody (Abcam, ab98941) or CD4 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: Chromatin was prepared in duplicate from MOLM-13 cells (6 sonication cycles, 30”ON/30”OFF) and immunoprecipitated using anti-H3K4me3 (Ab8580, Abcam, UK) or anti-H3K36me3 (C15310058 ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Microbiology 2022Quote: ... Validation of host targets for specific cytokines (IL-1β, IL-6, KC, and TNF-α) were performed by sandwich ELISA (AbCam) as indicated in our prior study (46).
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... 72 h post-transfection cells in 6 well format were harvested in 1% SDS to assess knockdown efficiency via immunoblot (Abcam, #ab134113).
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bioRxiv - Biochemistry 2024Quote: ... the wells were washed 6 times with wash buffer and then incubated with 100 µL per well of mouse biotinylated anti-DDDDK (Abcam ab173832) at 1:3000 in dilution buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Mitochondrial membrane potential was assessed using the TMRE (tetramethylrhodamine, ethyl ester) assay kit (Abcam, ab113852) in nonquench mode ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial membrane potential was measured using tetramethylrhodamine ethyl ester (TMRE; Abcam, 100 nM, 20 minutes), and ROS production was evaluated with MitoTracker™ Red CM-H2Xros ...
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bioRxiv - Developmental Biology 2021Quote: ... The mouse anti-DP Yun (#6, 1:5 (Du et al., 1996) and the mouse beta actin (1:1000, Abcam, Cat#ab8224) antibody was used as loading control in western blot assays.
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bioRxiv - Genomics 2022Quote: ... Cells were collected and incubated with antibodies against Anti-Mouse Integrin alpha 6 antibody (GoH3) conjugated to PE (1:600) (Abcam, Cat#: ab95703), a 1:200 CD104 antibody conjugated to FITC (Biolegend ...
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bioRxiv - Pathology 2020Quote: ... We used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich and proliferating cell nuclear antigen (PCNA, clone PC10, Abcam) for detecting mitotic spindles and proliferating cells ...
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bioRxiv - Immunology 2020Quote: ... 96-well ELISA plates were coated overnight with 1 µg/well of polyclonal rabbit anti-ESAT-6 Ab (#ab45073, Abcam Inc, USA) followed by blocking with 2% BSA for 1 h at 37° C or overnight at 4° C ...
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bioRxiv - Cell Biology 2021Quote: ... 4μm liver sections were retrieved for 25 minutes in Citrate buffer (pH 6) and were incubated with antibodies against GFP (Abcam, ab13970, 1:500), RFP (Rockland ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by heat-based antigen retrieval in citrate buffer (pH 6) and primary antibody incubation (anti-ATP6V1B1, Abcam ab192612, 1:500) diluted in PBS/1%BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in goat serum for 30 min and incubated overnight at 4°C with primary antibodies against DOT1L (Abcam, ab64077, 6 µg/ml), HIF-1α (Abcam ...
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bioRxiv - Genetics 2020Quote: Mitochondrial membrane potential was measured by flow cytometry in HEK293 cells using TMRE (Tetramethylrhodamine, Ethyl ester, Abcam, ab113852). FCCP was used as a positive control to validate each independent experiment ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections at the level of the PnC of GlyT2-eGFP mice injected with pAAV DJ-CamKIIα-mCherry in the CeA (N=6 mice) were incubated with a chicken anti-mCherry (1:1000, Abcam, catalog# ab205402, lot# GR225123-3) and a rabbit anti-PSD95 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 µM Pitstop-2 (Abcam), hypotonic medium (80% of medium was replaced with water ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2; ab196883) and monoclonal antibodies against phospho-ERK1/2 (p-ERK1/2; ab201015) were purchased from Abcam. Protein expression was normalized to GAPDH (sc-2577 ...
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bioRxiv - Immunology 2021Quote: ... 2-N-nitrobenz-2-oxa-1,3diazol-4-amino]-2 deoxy-D-glucose (2NBDG) (Abcam). CD4+ cells were cultured for 24h in anti-CD3 coated plates (0.5μg/ml ...