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Citations for abcam :
251 - 300 of 305 citations for Boc 6 fluoro DL tryptophan since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... for Im7 variants or 50 μL of 1:5,000-diluted HRP-conjugated anti-6×His tag antibody (Abcam, catalog # ab1187) for scFv-HER2 variants ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated for 6 hours on a rotating wheel at 4°C with primary antibodies MTHFD2 (Abcam; #ab151447; 5 μg) or negative control IgG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The equivalent of 3-6 x107 cells were precipitated overnight with 1 ug IgG/106 cells of rabbit antibody against H3K27ac (ab4729, Abcam). Before antibody precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Neuroscience 2024Quote: ... coli), monoclonal Xarr1-6 anti-SAG (Peterson et al., 2003) and monoclonal G8 anti-GRK1 (Zhao et al., 1998)(Abcam). Images were obtained using a Zeiss 510 meta confocal microscope equipped with a 40X N.A ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... an sIPSC baseline was recorded for 6 minutes and compared to sIPSCs after 20 minutes wash in of 1 μM TTX (Abcam), 0.2 μM K252a (Tocris) ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Immunology 2022Quote: ... Each well of a 6-well plate was incubated for 30min at room temperature with 200μL of Cell Extraction Buffer PTR (ab195215, ABCam, UK) containing phosphatase inhibitors and the protease inhibitor aprotinin ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBST and stained for 1 hour with primary antibodies (ZEB1: Bethyl, IHC-00419; E-Cadherin: BD Biosciences, 610181; Collagen 6: Abcam, ab182744 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Physiology 2024Quote: We utilized two commercially available enzyme-linked immunosorbent assay (ELISA) kits for the quantitative analysis of IL-6 (Abcam, ab100713) and IL-8 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-mouse IgG H&L (6 nm Gold) (CAT NO. ab39614) and anti-TSG101 antibody (CAT NO. ab30871) were purchased from Abcam. Alexa Fluor 568 goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed using the confor-mation-specific α-synuclein antibody MJF-14 (MJFR-14-6-4-2; Abcam, #ab214033) or the total α-synu-clein antibody syn211 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: Dot blots were used to confirm immunoreactivity of generated PFFs with a commonly used conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam). 1.5 µl of serially diluted monomer or (un)sonicated PFF samples were loaded onto nitrocellulose membrane (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was quantified by dot blot (described above) using a conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam), and diluted PFFs as a standard series ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hrs at room temperature before incubation with primary antibody (1:500, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in blocking solution overnight at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... tissue sections were incubated in boiling citrate buffer with 0.05% Tween 20 at 95°C (pH 6) for 20 minutes or treated with Pepsin C (Cat. No. ab64201; Abcam) for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Physiology 2021Quote: ... If not otherwise mentioned heat induced antigen retrieval with citrate buffer (pH 6) was performed prior to incubation with the following rabbit mono or poly-clonal antibodies: UCP1 (Abcam ab10983) 1:800 ...
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bioRxiv - Cell Biology 2022Quote: Epidermal punch biopsies (6 mm diameter) in triplicates were floated on medium containing either a mouse α-RTN1A ab (mon162, abcam) or the respective isotype control ab (IgG1 ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Immunology 2022Quote: ... slides were performed on the automaton Leica Bond RX and unmasked at pH 6 before incubated 30 minutes with an anti-CD8 antibody (Abcam, ab98941) or CD4 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: Chromatin was prepared in duplicate from MOLM-13 cells (6 sonication cycles, 30”ON/30”OFF) and immunoprecipitated using anti-H3K4me3 (Ab8580, Abcam, UK) or anti-H3K36me3 (C15310058 ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586; anti-N2D, Millipore, MAB5578; anti-strep, Abcam, ab252885; anti-6×his, Abcam, ab15149) and subsequently with secondary antibodies of HRP-conjugated (mouse IgG ...
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bioRxiv - Microbiology 2022Quote: ... Validation of host targets for specific cytokines (IL-1β, IL-6, KC, and TNF-α) were performed by sandwich ELISA (AbCam) as indicated in our prior study (46).
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... 72 h post-transfection cells in 6 well format were harvested in 1% SDS to assess knockdown efficiency via immunoblot (Abcam, #ab134113).
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bioRxiv - Biochemistry 2024Quote: ... the wells were washed 6 times with wash buffer and then incubated with 100 µL per well of mouse biotinylated anti-DDDDK (Abcam ab173832) at 1:3000 in dilution buffer overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The IP was performed using Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (Abcam, ab108123; 3μg/2 X 10^6 cells) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The mouse anti-DP Yun (#6, 1:5 (Du et al., 1996) and the mouse beta actin (1:1000, Abcam, Cat#ab8224) antibody was used as loading control in western blot assays.
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bioRxiv - Cancer Biology 2020Quote: ... with fluorochrome for 1 hour in the dark and the samples were then mounted with Fluoroshield mounting medium with DAPI (4’,6-diamidino-2-phenylindole, Abcam, Cambridge, MA). Slides were imaged using the Keyence BZX-700 Fluorescent Microscope (UCSD Microscopy Core).
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bioRxiv - Cell Biology 2021Quote: ... lysates of transduced cortical neurons cultured in 6-well plates were were incubated overnight with 2 µl of rabbit α-AP-3µ1 antibody (Abcam, Reference ab201227) on a rotating wheel at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Cells were collected and incubated with antibodies against Anti-Mouse Integrin alpha 6 antibody (GoH3) conjugated to PE (1:600) (Abcam, Cat#: ab95703), a 1:200 CD104 antibody conjugated to FITC (Biolegend ...
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bioRxiv - Pathology 2020Quote: ... We used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich and proliferating cell nuclear antigen (PCNA, clone PC10, Abcam) for detecting mitotic spindles and proliferating cells ...
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bioRxiv - Immunology 2020Quote: ... 96-well ELISA plates were coated overnight with 1 µg/well of polyclonal rabbit anti-ESAT-6 Ab (#ab45073, Abcam Inc, USA) followed by blocking with 2% BSA for 1 h at 37° C or overnight at 4° C ...
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bioRxiv - Cell Biology 2021Quote: ... 4μm liver sections were retrieved for 25 minutes in Citrate buffer (pH 6) and were incubated with antibodies against GFP (Abcam, ab13970, 1:500), RFP (Rockland ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by heat-based antigen retrieval in citrate buffer (pH 6) and primary antibody incubation (anti-ATP6V1B1, Abcam ab192612, 1:500) diluted in PBS/1%BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used and dilution ratios are: mouse monoclonal anti-Acetylated α-Tubulin (1:500, ab24610, clone 6-11B-1, abcam, Cambridge, MA), mouse monoclonal anti-Acetylated α-Tubulin (1:500 ...
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bioRxiv - Pathology 2020Quote: ... antigen retrieval at pH 6 with citrate buffer and the primary antibody rabbit anti-mouse CD11b (1:200) (ab133357, Abcam, Cambridge, MA, USA) and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100 ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in goat serum for 30 min and incubated overnight at 4°C with primary antibodies against DOT1L (Abcam, ab64077, 6 µg/ml), HIF-1α (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxy-6-pentadecyl-benzamide (CTPB) (CAS #: 586976-24-1) (>99% purity) was purchased from Abcam (Cambridge, United Kingdom). For both chemicals ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining ~800 µL was divided into 2 tubes to incubate with either 2 µL anti-H3K9me2 (Abcam 1120, Lot No. 1009758-6) or 1 µg anti-H3K9me3 (Diagenode ...
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bioRxiv - Biochemistry 2024Quote: ... was used as capture antibody and 5nM of Alexa-Fluor 561 labeled pSyn specific antibody and 5nM of Alexa-Fluor 637 labeled Anti-αSyn aggregate antibody [MJFR-14-6-4-2] (Abcam, Cat. No. ab214033) served as detection probes ...