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Citations for abcam :
2851 - 2900 of 4975 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry was performed for neuroblast detection and analysis of hippocampal neurogenesis using the following primary antibodies diluted in PBS containing 5% goat serum and 0.1% Triton-X-100: anti-doublecortin (DCX, rabbit: 1:5000, Abcam, #ab18723), anti-bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used with a total of 5 µg of each primary antibody used per immunoprecipitation: Rabbit anti-LSD1 (ab17721, Abcam), Rabbit ani-MYC (13987 ...
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bioRxiv - Cell Biology 2022Quote: ... Anti-Cullin 5(ab184177), anti-Elongin B antibody (ab154854, 1:2000) and anti-SOCS2 antibody (ab109245, 1:1000) were purchased from Abcam. Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech ...
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Evaluation of the DBA/2J mouse as a potential background strain for genetic models of cardiomyopathybioRxiv - Physiology 2022Quote: ... blocked in 5% BSA-PBS at room temperature and incubated with the anti-syntrophin primary antibody (1:2000; #11425; Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517 ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
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bioRxiv - Physiology 2023Quote: ... This was followed by placing in 5% normal goat serum for 1h and labelling for mCherry with rabbit anti-mCherry (1:10,000; Abcam; RRID:AB_2571870) and goat anti-rabbit 568 (1;400 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
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bioRxiv - Developmental Biology 2023Quote: ... For anti-PI4KIIIalpha staining, the blocking buffer (for blocking, primary, and secondary steps) was supplemented with 5% Normal Goat Serum (Abcam).
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bioRxiv - Microbiology 2022Quote: ... blocked for 1 h using 5% donkey serum and then incubated with a rabbit monoclonal H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) at 1:500 dilution overnight in a humidified chamber at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the membrane was incubated with the rabbit anti-IMPA1 antibody (1:10000 diluted in 5% BSA in 0.1% TBST) (Abcam ab184165) or goat anti-CHRM1 antibody (1 µg/ ml ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were rehydrated with PBS for 5 minutes before blocking and permeabilization for 1 hour with 10% normal goat serum (Abcam) containing 0.3% PBS-TritonX-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked using TBS-T with 5% BSA for 1 hour at room temperature and incubated using p-SMAD3 (ab52903, Abcam), type I collagen (1310-30 ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times with PBS for 5 min each and incubated with secondary antibody Alexa Fluor® 647 (Abcam, ab150067) for an hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...
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bioRxiv - Immunology 2023Quote: Paraffin sections (5 μm) from lung tissues were processed using standard protocols for immunohistochemistry (IHC) staining according to manufacturer’s instructions (Abcam: ab236468). Tissues were permeabilized by incubation in 0.4% Triton-X in TBS for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... was used to separately conjugate an anti-tau antibody (Tau-5, AB_2721194) and anti-α-synuclein antibody (MJFR1, Abcam, ab138501) to dibenzocyclooctyne (DIBO ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h prior to incubation in primary antibody in TBST with 5% skim-milk (rabbit anti-GFP 1:10,000; Abcam - ab290) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
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bioRxiv - Cell Biology 2023Quote: ... sections were blocked with 10% goat serum and then stained with Keratin 5 or Keratin 8 together with GFP antibody (Abcam) in 10% goat serum for overnight at 4□ ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% donkey serum in 1x PBS for 1 hour and incubated with UFM1 (Abcam 1:100) and MC1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2024Quote: ... was detected in A549 cells fixed with 1% paraformaldehyde for 5 min using an anti-MT1-MMP rabbit monoclonal antibody (1:100 dilution, EP1264Y, Abcam). F-actin was stained with Alexa Fluor 350- or 568-conjugated phalloidin (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h at room temperature in 20 mL TBS-T with 3% milk powder and 1:5,000 diluted anti-GFP antiserum (Abcam) while shaking with 180 rpm ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50; rabbit anti-Tuj1, Abcam, ab18207 at 1:1000). Tissues were washed for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged ribosomes were collected by indirect IP by adding 40 ug of anti-HA antibody (Abcam ab9110, RRID:AB_307019) to CB lysate pools and 5 ug to NP lysate pools ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked with 3% FBS in PBS for 20 min and then incubated 30 min with 0.3 ug of anti-GLUT1 (clone EPR3915) (Alexa Fluor 647 conjugated, Abcam_ab195020) on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with respective assay diluents from each kit to 2.5 μg/ml in 50 μL for the GFAP ELISA kit (Abcam ab233621), 50 μg/ml in 50 μL for the S100B ELISA kit (Abcam ab234573) ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were treated with 7-Chlorokynurenic acid (7-CKA) (final concentration 50 μM; 1 μL stock solution in DMSO in 1 mL media; ab120024; Abcam) and D-AP5 (final concentration 100 µM ...
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bioRxiv - Cancer Biology 2021Quote: ... Multi-color immunohistochemistry was conducted on a cohort of early-stage non-small cell lung cancer patients as described elsewhere (https://jitc.bmj.com/content/9/2/e001469) with polyclonal rabbit anti-Neutrophile Elastase antibody (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... Immunohistochemistry was executed on human colonic tissue using antibodies against Epac-2 and Rap-1 (1:100; Abcam).
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bioRxiv - Cell Biology 2020Quote: ... Western blot analysis was performed using 100 μg of total protein using anti-ACE2 (Abcam/ab239924/EPR4435(2)) ...
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bioRxiv - Genetics 2021Quote: ... Lysates were precleared and then immunoprecipitated with 2 μl anti-histone H3 (monomethyl K9) antibody (Abcam no. ab9045), 2 μl anti-histone H3 (dimethyl K9 ...
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bioRxiv - Cancer Biology 2020Quote: ... pancreatic or lung CAFs were treated with an inhibitor to beta-site Amyloid precursor protein cleaving enzyme 1 and 2 (BACE) (0.7μM; Abcam) for the duration of CAF CMed generation to inhibit Amyloid β production by the cells.
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846 ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...