Labshake search
Citations for abcam :
2801 - 2850 of 2957 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The XCL-1 measurement in mouse blood plasma samples was performed by using Mouse XCL- 1 SimpleStep ELISA kit (Abcam). The assay was performed as per manufacturer guidelines ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability of SHANK3-silenced PANC-1 cells treated with ERK and MEK inhibitors were measured using Cell Counting Kit-8 (WST-8 / CCK-8; Cat. no. ab228554, Abcam). Cells were silenced for 24 h as described above and then seeded on a 96-well plate with full medium containing DMSO (control) ...
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bioRxiv - Genomics 2022Quote: We determined the cytomegalovirus (CMV) serostatus of AFB (n = 80) and EUB (n = 80) donors with a human anti-IgG CMV ELISA kit (Abcam) on plasma samples ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation of HeLa cells transfected with the ZC3H11A-Flag expressing plasmids (5μg plasmid/100mm tissue culture plate) was done using Cell Fractionation Kit (Abcam, ab109719) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Quantitative cAMP measurements were carried out on isolated cardiac mitochondria (0.5 mg per sample) using cAMP Direct Immunoassay Kit (Fluorometric) (Abcam, ab138880). Mitochondria samples were treated with 0 or 10 or 15 mM [HCO3−] ...
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... spent media was collected for each condition and lactate secretion was assayed in duplicate or triplicate using either Lactate Colorimetric/Fluorometric kit (abcam) or Lactate-Glo (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... ELISA was performed on all serum samples after day 20 to measure the serum TTR level using Mouse Prealbumin ELISA kit (Abcam).
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bioRxiv - Cancer Biology 2024Quote: ChIP experiments were performed in duplicate for WT and MT cells (clones MT1 and MT2) using the High Sensitivity ChIP Kit (Abcam). 5x105 cells were used as input material for each immunoprecipitation ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: The amount of α-synuclein in the culture medium was measured using a mouse α-synuclein ELISA kit (Abcam ab282865) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and native histones that contain the modification and were purified from HEK293T cells using a Histone Extraction Kit (Abcam ab113476). Protein concentration was determined using the Pierce BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...
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bioRxiv - Cancer Biology 2023Quote: ... PSA levels were quantified both in the conditioned media and in the mouse sera using the PSA ELISA kit (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated with the polymer rabbit detection system (DCS) and phH3 staining was detected after incubating the slides with DAB substrate kit (Abcam) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: C3 or C5 levels in feces or serum of mice were measured with a Mouse C3 ELISA kit (Abcam, ab157711) or a Mouse C5 ELISA kit (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative detection of IgM/IgG in exosomes was performed according to the manufacturer’s instructions (IgM/IgG cow ELISA kit, Abcam, UK). The absorbance was determined on a plate reader using the contents of each well at 450 nm ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-1D4 Ab and anti-OLLAS Ab were conjugated to PE using an Ab conjugation kit from Abcam (cat. 102918) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was then centrifuged at 1500 x g for 10 minutes and plasma was collected and analyzed with a TAT ELISA kit (Abcam) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: Cell apoptosis resulting from the treatment with carbonic anhydrase inhibitor – S4 was evaluated using the Annexin V-FITC Apoptosis Staining/Detection Kit (ab14085) according to the manufacturer’s protocol (Abcam Inc.). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Separation of U2OS cellular compartments was performed according to the manufacturer’s instructions with a Standard Cell Fractionation kit (abcam # ab109719). Two different markers were used to assess the purity of each fraction ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and nuclear PPARα in V1 were measured using ELISA kits according to manufacturer’s instructions (total AMPK: ab181422, Abcam, UK; phosphorylated AMPK: KHO0651, ThermoFisher, UK; PPARα: ab133107, Abcam, UK). For tissue collection ...
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bioRxiv - Neuroscience 2024Quote: ... Plasma GLP-1 concentrations were analyzed by an experimenter blinded to treatment conditions in the DRC Radioimmunoassay and Biomarkers Core at Penn using the Human GLP-1(7-36) ELISA Kit (ab184857; Abcam) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... purified Lp(a) preparations were treated with a variety of fluorescent labeling strategies – FITC Conjugation Kit (Abcam, Cambridge UK, #ab188285), Dil (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate ...
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bioRxiv - Pathology 2024Quote: ... microsome were isolated from the livers of control (Mboat7fl/fl) or hepatocyte-specific Mboat7 knockout mice (Mboat7HSKO) using microsome isolation kit from Abcam and protein was quantified using the bicinchoninic assay (Pierce) ...
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bioRxiv - Molecular Biology 2024Quote: Competition-EMSA was done as described previously (Jin, et al. 1999) with JEG-3 cell nuclear extract isolated using the EpiSeeker nuclear extraction kit (Abcam). Nuclear extract (3 μg) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the supernatant was diluted 1:25000 or 1:50000 and quantified using an Albumin pig ELISA kit (Abcam, Cambridge, UK) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... followed by incubation with a secondary antibody (Mouse and Rabbit Specific HRP/DAB Detection IHC kit, ab64264, Abcam Limited., USA) and hematoxylin ((HWI-125 ...
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bioRxiv - Biophysics 2024Quote: ... bovine serum albumin (BSA) was removed (when applicable) from the stock antibody solution with an antibody conjugation kit according to manufacturer instructions (Abcam). After BSA removal ...
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bioRxiv - Cell Biology 2024Quote: ... The fixed cells were stored at 4°C until all time points were harvested before proceeding with DNA staining and FACS analysis with the propidium iodide flow cytometry kit for cell cycle analysis (ab139418 from Abcam), following the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2024Quote: Quantitative estimation of cholesterol in the plasma and tissue lysates was undertaken with the aid of a commercially available Cholesterol Quantification Kit (abcam) as per the provided protocol ...
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bioRxiv - Cancer Biology 2024Quote: The apoptosis assay was performed using FACS using Annexin V–CF Blue/7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit (ab214663, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... An ELISA kit was used to quantify the total amount of β-catenin according to the manufacturer’s instructions (ab275100, Abcam). Samples were diluted to a concentration of 300 µg total protein/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were baked at 65°C for 1 hr and de-paraffinized by xylene then rehydrated and stained with a trichrome stain kit (Abcam). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: Levels of anti-inflammatory cytokine Transforming growth factor-β1 (TGF-β1) in supernatants were measured by an ELISA kit (ab119558, abcam, UK), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...