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Citations for abcam :
2551 - 2600 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... slides were incubated with 1° antibody for CD31 (ab28364, Abcam, 1:25 dilution) or VE-Cadherin (ab205336 ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated for 1 h at room temperature, CD4 (ZM0418, clone UMAB64, Zsbio, 1:200) and CD133 (ab19898, polyclonal antibody, abcam, 1:400) were incubated for overnight at 4□.
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Antibodies in fresh 5% NFDM were added (Mouse monoclonal GAPDH 1:10,000; Goat polyclonal ACE2 [R&D Tech AF933] 1:200; Rabbit monoclonal BRD2 [abcam 197865] 1:5,000) and incubated at 4 °C for at least 16 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1:500 dilution) and anti-tissue inhibitor matrix metalloproteinase 1 (TIMP1, at 1:1000 dilution) antibodies were all from Abcam (Branford, CT). After incubation with primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr before incubation with primary antibodies (mouse anti-tyrosine hydroxylase 1:1000, AB152; rabbit anti-mCherry 1:1000, ab167453; chicken anti-GFP 1:1000, ab13970; Abcam, Cambridge, UK) in blocking solution at room temperature on a shaker for 18 hr ...
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bioRxiv - Physiology 2021Quote: ... Moreover the neuronal spheres were immunostained for neuronal stem cell markers: Nestin (1: 500; DSHB, Iowa, USA; 77, Foxg-1 (1:100; Abcam, Cambridge, UK), Emx1 (1 ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2021Quote: ... sc84663 [santa-cruz], 1/1000 dilution), anti-Pol2tot (rabbit, A300-653A [Béthyl] 1/400 dilution, anti-Pol2Ser2P (rabbit, ab5095 [abcam] 1/100 dilution) and anti-Pol2Ser5P (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... were subjected to western blotting using the indicated primary (mouse anti-GABAA receptor alpha 1 #ab3299, Abcam, 1:200; rabbit antibeta actin #ab8227, Abcam, 1:1000) and secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used included those against α-tubulin (T5168, Sigma, ascites fluid, 1 in 5000 and ab4074, Abcam, 1 μg ml-1) polyglutamylated tubulin B3 (T9822 ...
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bioRxiv - Genetics 2022Quote: ... 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233, 1:100; Anti-GAPDH rabbit, Abcam ab9485, 1:1000), 3x 5-min washes with PBS-T ...
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bioRxiv - Cell Biology 2024Quote: The primary antibodies are as follows: anti-NOX2/gp91phox (ab129068, dilution 1:400), anti-ACTB (ab8227, 1:2000), anti-OCN (ab93876, 1:500) from Abcam (Cambridge, UK); anti-SOD2 (ADI-SOD-111 ...
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bioRxiv - Immunology 2024Quote: ... anti-beta Actin HRP [AC-15] (#ab49900, diluted at 1:10000), anti-IL-1 beta (#ab2105, diluted at 1:1000) antibodies from Abcam (Cambridge, UK), anti-AMPKα2 (#AF2850 ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed for the HLA class I heavy chain (HCA2, 1:1000, Nordic Bio, MUB2036P; HC10, 1:3200, Nordic Bio, MUB2037P) and β-2m (EPR21752-214, 1:4000, Abcam, ab218230) on all generated TMAs as described previously42 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Ectopic huLiver tissue was processed and stained using the methods described above with polyclonal rabbit anti-MSP1 (1:100) and monoclonal mouse anti-EBA-175 (1:100) followed with goat anti-rabbit IgG (1:100) (Abcam, Cambridge, UK), and goat α-mouse IgG1 (1:100 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used were (H3K27me3 Cell Signaling C26B11 1:500, EZH2 Cell Signaling 5246S 1:200, B2M Millipore MABF1968 1:2000, HLA-DR,DQ,DP AbCAM ab7856 1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham, MA, USA; anti-laminin, ab11575, 1:200, Abcam, Cambridge, UK) diluted in 1% BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.05% Tween 20 in PBS for 1 hr followed by incubation in primary antibodies ((MYC 1:100, Abcam Cat# 32072, γH2AX 1:100 Abcam Cat# 303656) for overnight at 4°C in a humid chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked in 5% normal serum in 1× PBS Triton 0.3% for 1 hr at room temperature and then incubated with the primary antibody (anti-S100b diluted 1:1000, Abcam, ab5264 or anti-RPE65 diluted 1:250, Thermo Fisher Scientific, MA1-16578; anti-CD34 diluted 1:200, abcam, ab8158) diluted in blocking solution for 1.5 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in PBS for 5 min before being blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Neuroscience 2022Quote: ... spinal transverse sections 5 mm caudal and rostral from the lesion center were prepared and stained for BDA or PKCγ (Abcam, ab109539). For mice with uPx ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked with 5% BSA in PBS for 30 min at room temperature and incubated with anti-Rad51(abcam ab133534) primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μM ThT in the presence or absence of purified recombinant NRN1 (5 μg/mL or 263 nM; Abcam, ab69755) protein ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were washed three times with PBS following fixation and blocked with blocking buffer: 5% normal goat serum (NGS, Abcam, AB7681) in PBS with 0.1% Triton-X (PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [18] ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of total fragmented chromatin was saved as an input control and the remaining chromatin was split into two tubes (representing 5×107 cells each) for antibody pulldown and incubated overnight at 4°C with 15 ug of NRF1 (Abcam #ab55744), or MYC (Abcam #ab56 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations: α-smooth muscle actin (αSMA, mouse, Cat. No. ab7817, Abcam) at 1:300 dilution ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with blocking buffer (5% goat/mouse serum in 0.3% triton-x100 in 1xPBS) and stained with rabbit anti-human CD3ζ (abcam cat# Ab226263). Coverslips were then gently washed 3 time with 1x PBS for 10 minutes each and stained with Donkey anti- Mouse IgG Antibody ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then treated with 5% BSA in PBS for 90 minutes at 25°C followed by treatment with anti-Listeria antibody (Abcam: ab35132) at 25°C for 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... 800 µl of chromatin was mixed with 3200 µl of chromatin dilution buffer and 5 µg of rabbit polyclonal anti-V5 (Abcam, ab15828) or normal rabbit IgG (Merk ...