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Citations for abcam :
2451 - 2500 of 2527 citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407 ...
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bioRxiv - Cell Biology 2022Quote: ... The relative amount of each of these muscle proteins in each lane was normalized to the amount of Histone H3 detected using anti-Histone H3 (abcam, cat. ab1791) at 1:40,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 1 μg of a specific α2 AMPK antibody (ab3760, Abcam, Cambridge, MA) coupled with protein G-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Electroporated cells were seeded in StemMACS iPS-Brew XF with Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM, Abcam, Cambridge, UK) and 1x CloneR (Stemcell Technologies ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... They were then washed with PBS and incubated with 1° antibodies (α-NDUFV2, 15301-1-AP, Protein tech, α-tubulin, Abcam, ab179513) in 5% milk 0.1% Tween 20 ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429; Abcam, Cambridge, UK) at 1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Systems Biology 2024Quote: ... was conducted to normalize protein concentrations between all samples and hexokinase activity was measured according to the manufacturer’s protocol (Abcam, Cambridge, MA; ab136957), using 10 μg of protein per condition ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595, 1:600; anti-GM130, Abcam, ab52649 ...
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bioRxiv - Genomics 2024Quote: ... we extracted the total protein from the mutant cells and conducted western immunoblotting using a recombinant anti-HA tag antibody (Abcam, Waltham, MA).
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The sample were incubated for 48 hrs at 4°C for secondary antibodies Alexa Fluor 647 (Donkey anti-Rabbit, Abcam ab 150075, 1:500 dilution) and Alexa Fluor 555 (Anti-mouse IgG (H+L) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Physiology 2020Quote: ... Blots were probed using an antibody against one subunit of each of the mitochondrial oxidative phosphorylation complexes (Abcam #ab110412; 1:1000; overnight at 4°C) and followed by fluorescent secondary (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ChIP-seq was carried out using 30 μg of chromatin isolated from cryopreserved PBMC samples obtained from two CLL patients and 4 μl of anti-EGR2 antibody (Abcam, cat# ab43020, Lot# GR101477-1). The immunoprecipitated DNA was processed into a standard Illumina ChIP-seq library and sequenced on an Illumina HiSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies used were for MHC (Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA; 4 μg/mL) and Phalloidin-488 (Abcam #ab176753, Cambridge, UK; 1:1,000). Following primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000, anti-GAPDH; Abcam ab9485 1:10000, Cambridge, UK) 3x 5 minute washes with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% w/v skimmed milk in TBST for one hour and then incubated at 4□ with primary antibodies (p-PI3K, Abcam, Catalog#ab278545; p-AKT(S473), CST ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...
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bioRxiv - Cell Biology 2019Quote: ... with 2-8 μg total protein loaded per lane and the following dilutions of mouse mAbs: anti-K14 (1:500; clone LL002, Abcam Inc., Cambridge, UK), anti-K42 (1:500) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
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bioRxiv - Plant Biology 2021Quote: ... For each ChIP/re-ChIP assay, 20 μg DNA/protein complexes were immunoprecipitated with anti-H3K27me3 (Milipore, Cat No 07-449) and anti-H3K18ac (Abcam, Cat No ab1191) antibodies using the Re-ChIP-IT kit (Active Motif ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were then processed through an ELISA based kit for detecting p65 (NF-κB p65 Transcription Factor Assay Kit, abcam, Cambridge, MA, #ab133112). Absorbance at 450 nm was read with SpectraMax iD3 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam, Cambridge, Great Britain) as described previously [64] ...
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bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
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bioRxiv - Bioengineering 2019Quote: ... Tangential cortical sections of 30 μm-thickness were sliced using a cryostat and directly mounted on gelatin-subbed glass slides for immunohistological staining of GFAP (glial fibrillary acidic protein, Abcam, Cambridge, MA, USA) for astrocytes ...