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Citations for abcam :
2451 - 2500 of 2642 citations for 4 METHANESULPHONYLBUTAN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The medium in each well was replaced with HBSS with 10 μM DCF-DA (2’,7’-dichlorofluorescein diacetate; D6883, Abcam) or propidium iodide staining solution (P4864 ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Developmental Biology 2023Quote: ... BSA (blocking agent) was applied on slides for 35 minutes followed by overnight incubation with primary antibodies [MMP-2 (Abcam) at a dilution of 1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), BUBR1 (rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in PBS and incubated for 2 hr at room temperature with secondary antibodies 594-donkey-anti-rabbit (1:50; Abcam) and 488-donkey-anti-chicken (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003; H3, Abcam ab1791; Normal Rabbit IgG, Abcam ab37415) were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
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bioRxiv - Cell Biology 2023Quote: ... ab199956), Bcl-2 antibody (1:1000, rabbit anti, ab194583), and gp91phox (1:2500, rabbit anti, ab129068) were purchased from Abcam company ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for chromatin immunoprecipitation: rabbit anti-NK Homeobox 2-1 (NKX2-1, 1 µg per reaction, ab133737, Abcam) and rabbit anti-CEBPA (C/EBPα ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies and respective concentrations used in this study are the following: anti-Ki67 (2 µg/mL, ab15580, abcam), anti-p21Cip1/Waf1 (0.48 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: The second subset of islets was evaluated for oxidative stress by 2’,7’-Dichlorodihydrofluorescein diacetate (DCFDA) (Abcam, cat. no. ab113851) staining of reactive oxygen species (ROS) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the sections were incubated with primary antibodies SOX 2 Mouse (Sta. Cruz) with a dilution of 1:200 and Ki-67 rabbit (Abcam) with a dilution of 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The sample were incubated for 48 hrs at 4°C for secondary antibodies Alexa Fluor 647 (Donkey anti-Rabbit, Abcam ab 150075, 1:500 dilution) and Alexa Fluor 555 (Anti-mouse IgG (H+L) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were applied in blocking buffer at 4°C over-night (alpha-tubulin, Abcam ab233661, 1:1000 dilution and Pcdh19, Abcam ab191198, in 1:200 dilution). After 3 wash steps in PBS-T (0.1% triton) ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ChIP-seq was carried out using 30 μg of chromatin isolated from cryopreserved PBMC samples obtained from two CLL patients and 4 μl of anti-EGR2 antibody (Abcam, cat# ab43020, Lot# GR101477-1). The immunoprecipitated DNA was processed into a standard Illumina ChIP-seq library and sequenced on an Illumina HiSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies used were for MHC (Developmental studies hybridoma bank #MF-20, Iowa City, IA, USA; 4 μg/mL) and Phalloidin-488 (Abcam #ab176753, Cambridge, UK; 1:1,000). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000, anti-GAPDH; Abcam ab9485 1:10000, Cambridge, UK) 3x 5 minute washes with PBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were again washed thrice in 0.1 M PB and then incubated with the secondary antibody for 2 h (goat anti-chicken-IgY conjugated to Alexa Fluor 594, Abcam, ab150172). Following the incubation with the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature (Chicken anti-GFP, Aves cat#GFP-1020, RRID:AB_10000240; Rabbit anti-NUP153, Abcam cat#ab84872, RRID:AB_1859766), followed by washing 3x 5 min in 1x PBS ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
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bioRxiv - Cell Biology 2021Quote: Total intracellular ROS contents of cultured HCs were measured using the fluorescent probe 2’,7’-dichlorofluorescin diacetate (DCFDA) (ab113851, Abcam, UK) (Lv et al. ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
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bioRxiv - Cell Biology 2022Quote: ... Epitope detection was conducted with a primary monoclonal mouse antibody against NONO at 1:500 dilution in PBST and a polyclonal rabbit antibody against RNA Pol II phosphorated at Serine 2 (Abcam, ab5095) at 1:500 dilution ...