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Citations for abcam :
201 - 250 of 285 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were then washed thrice with TBS-T and probed with secondary goat anti-mouse and anti-rabbit tagged with HRP (Abcam). The secondary antibodies were diluted in blocking solution and it was a 1/2000 dilution factor ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS-T (0.4% Triton X-100 in PBS) and incubated with antibody against GLUT-1 (1:200, Abcam, ab40084), CA3 (1:150 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies Vimentin diluted at 1:250 (MA5-11883, ThermoFischer Scientific) and Troponin T diluted at 1:500 (ab45932, Abcam) were added the next day and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... cultured osteoblasts or osteoclasts were treated with 25 ng/ml recombinant full-length human MMP-3 (Abcam, ab96555) in place of conditioned medium or in combination with conditioned medium.
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bioRxiv - Cell Biology 2021Quote: ... Homocysteine levels in supernatants containing 25 µg of protein were determined using the Homocysteine Assay Fluorometric Kit (Abcam) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... phospho-RIP3 (phospho S232) (EPR9516(N)-25) (ab195117, 1:1000), Raptor (EP539Y) (ab40768, 1:500) obtained from Abcam; and phospho-P62 (Ser351 ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-IRa (1:50 for Fig. 2B; 1:25 for Fig. 2C; ab1500 Abcam, Cambridge, MA). Dilutions were in in antibody dilution buffer (1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Sections were then washed in TBS and stained in 10% FBS in TBS-T (0.05% Tween-20) blocking solution with primary antibodies: anti-GFP 1:2000 (Abcam Cat# ab13970), anti-RFP 1:1000 (Rockland p/n ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Molecular Biology 2019Quote: ... solution and incubated for 1.5 h RT or overnight at 4° C with the primary antibodies in milk PBS-T (RNAP1: anti-RPA194, sc-48385, Santa Cruz; ACTβ: A5316, Sigma or ab8227, Abcam; NMI ...
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bioRxiv - Developmental Biology 2019Quote: ... Enzo Lifesciences #BML-PW8810), rat anti-Troponin-T (1:200, Barbarham Institute #BT-GB1455) and rat anti-Kettin (1:200, Abcam #ab50585). Alexa Fluor 488- ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed three times with PBS-T and then incubated with secondary HRP-conjugated anti-rabbit IgG (Abcam, Cambridge, UK) or anti-mouse IgG (Invitrogen™ ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice with PBS-T and incubated at 37°C with AlexaFluor goat anti-rabbit 488 (ab150077; Abcam Inc, Cambridge, MA) at 1:500 dilution for at 45 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Blots were then blocked with 5% skim milk in PBS-T for 1 hour and incubated with either rabbit anti-lamin B1 (1:1000, Abcam, ab16048), rabbit anti-dynamin 1 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed five times with PBS-T and 100 μL of TMB ELISA Substrate Solution (Abcam, Cambridge, MA, USA) was added to all wells of the plate and incubated for 5 minutes before the reaction was stopped ...
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bioRxiv - Cell Biology 2022Quote: ... permeabilized in PBS with 0.3% Triton X-100 (PBS-T) and stained with anti-F-actin iFluor 555 phalloidin antibody (Abcam, Cambridge, UK) to identify the cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked for 1 hr at RT in 3% BSA PBS 0.1% Tween-20 (PBS-T) and then incubated overnight at 4°C with primary antibody (rabbit anti-RGS9, 1:500 in blocking solution; Abcam, UK). After washing three times in PBS-T ...
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bioRxiv - Immunology 2022Quote: ... washed twice in TBS-T and incubated in the presence of a goat HRP conjugated anti-rabbit secondary IgG antibody (1:10000; Abcam 6721). Membranes were revealed using the Amersham ECL prime reagent (GE healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Embryos were washed (3x 30 min PBS-T) and then secondary antibody (goat anti-mouse Alexa Fluor® 555 - abcam ab150114) at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605 ...
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bioRxiv - Bioengineering 2020Quote: ... we also confirmed cardiac differentiation on dECM-fibrin samples and tissue culture plates using an antibody specific to cardiac troponin T (Abcam, ab8295). This data is provided in supplementary figure S1.
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated for 48 hours at 4 °C with primary antibodies diluted in 5% NGS in PBS-T as follows: rabbit anti-cFos (1:1000, Abcam, #190289) and chicken anti-GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Genomics 2023Quote: ... in PBS-T for 1 hour and incubated overnight in 5% goat serum in PBS-T with primary antibody at 4°C (SON (1:300, abcam ab121759) or SRRM2 (1:300 ...
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bioRxiv - Genetics 2023Quote: ... Slides were then washed with PBS-T once more before blocking for 1 hour at room temperature with 10% goat serum (abcam #ab7481) or 1% Bovine Serum Albumin (Sigma #05482 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubations were performed overnight at 4°C in PBS-T with 2% NGS plus one or more of the following antibodies: polyclonal rabbit anti-GFP (1:800, Abcam #ab290), monoclonal mouse anti-Abd-B (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS-T and 100 uL anti-hamster IgG horseradish peroxidase antibody (HRP, abcam, #ab6892) diluted 1:10,000 in PBS-T containing 1% milk powder was added to all wells ...
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bioRxiv - Physiology 2024Quote: ... antibody kindly gifted by Jan Nedergaard and Barbara Cannon) and anti-αTubulin to control for equal loading (1:1000 solution in 5% milk in TBS-T, Abcam 2144). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... anti-V5 tag (Thermo Fisher Scientific, R960-25; IB 1:5000, IP 1μg, and Abcam, Ab53418; IF 1:800), anti-GFP (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue slides were incubated with an anti-PD-1 monoclonal antibody (1:25 dilution, clone J116, Abcam, Cambridge, UK) or with an anti-PD-L1 monoclonal antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2021Quote: Direct binding between Rab5B and FHIP1B was assessed by incubating 15 μM 6xHis-SNAP-Rab5B with 25-molar excess of GTPɣS (Abcam) or GPD (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended in Buffer A with 0.1% BSA and then loaded with either anti-GFP polyclonal antibody (25 µg per IP, abcam) or anti-HA-tag monoclonal antibody (2.0 µg per IP ...
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Perivascular Excitation Tunnelling: a Novel and Preventable Cause of Cardiac Reperfusion ArrhythmiasbioRxiv - Physiology 2023Quote: ... Hearts were loaded with voltage-sensitive dye (25 µL bolus of 8.6 mM di-4-ANBDQPQ, Cytocybernetics, Buffalo, NY, USA) and electromechanically uncoupled ((±)blebbistatin ([Abcam, Amsterdam ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated for 1 h at 25 °C with IkB (Abcam, ab76429, 1:300 in 1% BSA/1X PBS) or TOMM20 (GTX ...
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bioRxiv - Microbiology 2021Quote: ... Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493). Blots were washed three times with TBST and once with TBS ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed with 1X TBS-T prior to incubating with HRP-conjugated goat anti-rabbit secondary IgG (ab6721, Abcam, Cambridge UK) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam, ab45932]). After washing cells once with PBS ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... in Tris-buffered saline-tween 20 (TBS-T) and were incubated with appropriate primary antibodies fibronectin (1:500; ab2413; Abcam, Cambridge, UK), collagen IV (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitations were performed with 1000 µg of extract and a 25 µl slurry of pan-mouse Dynabeads bound to anti-Myc (Abcam) or anti-V5 (Thermo-Fisher ...
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bioRxiv - Microbiology 2020Quote: Cells were infected with either WT or S mutants of SARS-CoV-2 at an MOI of 0.1 in the presence of 50 μM camostat mesylate (FUJIFILM Wako Pure chemical) and/or 25 μM E-64d (Abcam) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
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bioRxiv - Biochemistry 2021Quote: ... coli were purchased and added to Kinase Buffer I (25 mM MOPS pH7.2, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT; Abcam ab189135). Either water (mock ...
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bioRxiv - Cell Biology 2020Quote: Exosomes (25 μg prot) from A-375 cells were incubated or not with 5 µl anti-CD63 antibodies (Abcam, ab8219) for 2 h at room temperature ...