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Citations for abcam :
2401 - 2450 of 2785 citations for 3' Bromo 3 3 4 5 trifluorophenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The blocked membranes were further washed three times with TBST buffer and incubated at 4°C overnight with primary antibodies CDKN2A/p16INK4a (ab108349, Abcam, 1:1000), β-actin (Santa Cruz β-actin (C4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4 °C with primary antibodies prepared in blocking buffer against phospho-MLKL (Ser345) (Abcam, Cat. No. ab196436), MLKL (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized and then incubated with blocking solution (5% normal goat serum in 0.1% Triton X-100 in PBS) for 1 hr followed by primary antibody incubation overnight at 4°C using goat Anti-nNOS (ab1376, Abcam, 1:300) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then incubated overnight in humidified chambers at 4°C in the following primary antibodies diluted in blocking buffer: anti-MAP2 (1:700; Ab5392, Abcam, Cambridge, UK), SMI312 (1:250 ...
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bioRxiv - Cancer Biology 2020Quote: ... with fluorochrome for 1 hour in the dark and the samples were then mounted with Fluoroshield mounting medium with DAPI (4’,6-diamidino-2-phenylindole, Abcam, Cambridge, MA). Slides were imaged using the Keyence BZX-700 Fluorescent Microscope (UCSD Microscopy Core).
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and then incubated with the following primary antibodies (diluted in 5% non-fat milk in 0.1% PBS-Tween 20) for 18 hrs at 4°C: Mouse monoclonal Total OXPHOS rodent WB cocktail (Abcam ab110413, 1:1,000); mouse monoclonal Total OXPHOS blue-native WB cocktail (Abcam ab110412 ...
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bioRxiv - Systems Biology 2021Quote: ... prior to overnight treatment at 4 °C with rabbit anti-murine primary antibodies raised against GFAP (1:1000, ab7260, Abcam Ltd, UK) or Iba1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were then washed with PBS and incubated for 24 h at 4 °C with anti-BDNF (Abcam, USA, 1:400), anti-doublecortin (DCX ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in 2.5% BSA/PBS and cells were incubated overnight at 4°C in a humidified chamber with primary antibodies anti-GFP (Abcam, 1:5000), anti-GluA1 (homemade ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Then the tissue sections were incubated overnight at 4 degrees Celsius with the primary goat polyclonal to cfos (1:500, 24 h, abcam; RRID: SCR_012931) primary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... slides were blocked with PBS + 1% BSA + 2% goat serum + 0.2% Triton X-100 for 1h at RT and incubated overnight at 4◦C with anti-DCLK1 antibody (Abcam #ab31704; 1:250) in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated at 4°C in c-Fos primary antibody (1:4000 Anti-c-Fos rabbit pAb; 190289; Abcam, Toronto, ON) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sections were incubated for 72 h at 4 °C with gentle agitation with rabbit polyclonal anti GLS2 (ab113509; 1:1000, Abcam, Cambridge, UK) and sheep polyclonal anti pS6 (ab65748 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked for 30 min with 3% BSA dissolved in Tris-buffered saline with 0.1% Tween (BSA-TBST) and incubated overnight at 4 °C with primary antibody (ab43177 chicken-developed anti-ATP synthase β, Abcam plc) diluted 1:5000 in BSA-TBST ...
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bioRxiv - Immunology 2020Quote: ... The sonicated nuclear fractions were divided for input control and for overnight incubation at 4℃ with 10 μg of either anti-C/EBPβ (Abcam cat. 32358) or the negative control IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500, Covance; TH, 1:1000, Chemicon; Serotonin, 1:500, Abcam). Negative primary controls consisted of cells treated with blocking buffer without the addition of primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... The retinas were incubated in PBS with 1% Triton X-100 and 0.5% Tween 20 for 1 h at room temperature and in 4% BSA for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies: RBPMS (1:500, Abcam, Cambridge, UK), ChAT (1:100 ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Genetics 2022Quote: ... After 6 h the cells were washed in 1XPBS and fixed in 4% PFA followed by staining with rabbit polyclonal beta COP antibody (ab2899, Abcam, Cambridge, UK) after permeabilising with 0.5% Triton-X 100 ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Immunology 2022Quote: ... for 30 minutes at 4°C and then incubated with the titrated primary anti-MPO-PE and primary citrullinated histone H3 (citH3) antibody (Abcam, Waltham, MA) for 30 minutes at 4° C ...
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bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were incubated overnight at 4°C in 1:100 dilution of rabbit polyclonal anti-nitro tyrosine antibody (ab42789; Abcam, Cambridge, MA). Slides were rinsed in PBS and developed using polyclonal goat anti-rabbit Immunoglobulins conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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bioRxiv - Neuroscience 2023Quote: ... The embedding blocks were cut on a cryostat into 20 μm thick sections and blocked for 30 min with 4% BSA and then incubated for 1 hour with the following primary antibodies: polyclonal rabbit anti-RFP (1:200, Abcam, Cambridge, UK).
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bioRxiv - Pathology 2023Quote: ... membranes were incubated overnight at 4 °C with agitation with primary antibodies against acetylcholinesterase (68 kDa, 1:500, ab97299; Abcam, Cambridge, UK), butyrylcholinesterase (75 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... LiCor) for 1 hour at room temperature and probed overnight at 4℃ with a monoclonal rabbit anti-HTT antibody (Abcam: EPR5526; Ab_109115) diluted 1:1,000 in blocking buffer plus 0.2% tween ...
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bioRxiv - Physiology 2022Quote: ... primary antibodies were added in 50 μL blocking buffer for 12 hours at 4° C: ATP synthase antibody (Abcam, ab128743 1:200), sAC antibody (R21 IHC ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: rabbit anti-MAP2 (ab254264 Abcam, 1:2000), mouse anti-βTUBULIN III (TUJ1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated overnight at 4°C with anti-TOMM20 (1:200 dilution) and anti-cytochrome C (1:200 dilution) primary antibodies (Abcam, Waltham, MA). Thereafter ...
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bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... and incubated overnight at 4°C with the anti-F4/80 (#ab6640, 1:50) and/or anti-iNOS (#ab3523, 1:20) antibodies from Abcam (Cambridge, UK), and/or anti-AMPK alpha (Phospho Thr172 ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in a humid chamber overnight at 4°C in primary antibody: anti-GFAP (Abcam [ab4674], 1:200 dilution), anti-Iba1 (Abcam [ab178846] ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4) antibody (Abcam, ab8580; 1:50); anti-Histone H3(acetyl K27 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C overnight with primary antibodies including: NeuN (1:500, mouse, Millipore, Cat#:MAB377; 1:500, rabbit, Abcam, Cat#: ab104225), MBP (1:300 ...