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Citations for abcam :
2351 - 2400 of 3865 citations for Human Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Assay kits were obtained from Abcam, and the standardised protocol offered with the kit was followed ...
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bioRxiv - Cell Biology 2023Quote: Cellular ROS Assay kit (Abcam, ab186027) was used to check the oxidative stress according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A commercially available kit (Abcam ab138886) was then used to fluorometrically quantify hydrogen peroxide levels and was prepared according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: An autophagy assay kit (Abcam; ab139484) was used to measure autophagic vacuoles in live cells using a dye that selectively labels autophagic vacuoles ...
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bioRxiv - Bioengineering 2023Quote: ... The Masson’s trichrome staining kit (Abcam) was used following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... DAB Substrate Kit (Cat# ab94665, Abcam) was used for amplification and visualization of signal ...
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bioRxiv - Plant Biology 2024Quote: ... The EpiQuik Plant ChIP Kit (Abcam), anti-Flag (F3165-2MG ...
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bioRxiv - Cancer Biology 2024Quote: ... Quick proliferation assay kits (Abcam 65475) were also used for assessing proliferation.
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bioRxiv - Neuroscience 2024Quote: ... Fluorometric kits for adenosine (Abcam ab211094), inosine (Abcam ab126286) ...
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bioRxiv - Bioengineering 2024Quote: ... using the Comet Assay Kit (Abcam), and following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Hydroxyproline Colorimetric Assay Kit (Abcam, ab222941) was used to measure collagen in lung lobes following kit instructions and optical density was measured using a Varioskan LUX plate reader (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and 50 μL Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HSC70/HSP70 (clone N27F3-4, ENZO dilution 1:1.000, sample reduced with β-mercapthoethanol) and rabbit anti-human lactoferrin (polyclonal, Abcam, dilution 1:5.000), rabbit anti-human TLR3 (D10F10 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... The GP-13C6 complex was identified by adding a polyclonal human anti-IgG antibody conjugated to FITC (Abcam, Cambridge, USA; Cat. No. ab97224). Cell cultures were observed under fluorescence illumination with an optical microscope (Axio Imager M2 microscope (Zeiss ...
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bioRxiv - Physiology 2022Quote: ... and then stained for αSMA overnight using rabbit anti-human αSMA antibody at a 1:500 dilution at 4°C (Abcam, San Francisco, CA). The chamber slides were incubated with a goat anti-rabbit secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 μg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) for 30 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
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bioRxiv - Genetics 2019Quote: ... washed with 0.1% PBS-Tween for three 15min incubations and then incubated with rabbit-anti-human CR1 (also known as CD35; Abcam #ab126737, 1:100) for 48hrs in 0.1% PBS-Tween on an orbital shaker at +4°C ...
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bioRxiv - Pathology 2024Quote: ... Primary antibodies and labels used for wholemount staining in humans included mouse monoclonal anti-CD31 (1:50) and rabbit polyclonal anti-osteopontin (SPP1, Abcam, ab8448, 1:100). Rabbit polyclonal anti-osteopontin SPP1 ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... After washing with PBS a 2.5% BSA solution was applied for 1 hour and then incubated with primary anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903) and left over-night at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Human CSF samples were not lysed and free and total RIPK1 levels were separately detected with (Cell Signaling Technology, 3493 and abcam, ab125072). The assay was developed with 150 mL of 2x MSD read buffer (R92TC-3 ...
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bioRxiv - Biophysics 2024Quote: ... The primary antibodies were subsequently recognized by a goat anti-human IgG secondary antibody conjugated with DyLight® 650 fluorescent label (Abcam, Cambridge, UK). Cell nuclei were stained with DAPI (Beyotime ...
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bioRxiv - Neuroscience 2020Quote: ... This was done using a commercially available luminescence-based kit (ADP/ATP ratio kit, Abcam, cat.no. ab65313) following the user manual suggested by the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... ROS levels were measured by a cell-based fluorogenic assay kit (ROS/Superoxide detection assay kit; Abcam) as per the manufacturer’s protocol as previously described 95.
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bioRxiv - Microbiology 2020Quote: The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat. Ab28364, Abcam, Cambridge, UK) diluted 1:50 in the same blocking buffer ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Zoology 2020Quote: ... 0-3 days old) and older buds (stage 3, 1 month old) by two different means: anti Phospho-histone H3 (PHH3) immunolocalization (Abcam ab5176) antibody (1:200 ...
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bioRxiv - Microbiology 2019Quote: Immunostaining for p62 and ubiquitin was performed with blocking and permeabilization buffer (1% saponin, 3% bovine serum albumin) and anti-p62 rabbit monoclonal antibodies (Abcam #AB109012) at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2 ...
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bioRxiv - Physiology 2019Quote: ... samples were incubated overnight at 4°C with the following antibodies diluted in 3% BSA blocking solution and 1% horse serum: Anti-cTnT (Abcam, ab33589), anti-cTni (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The the membranes were incubated overnight at 4°C with rabbit anti-VEGFR-3 antibodies (Abcam, Cambridge, MA, USA; 1:50). Horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit antibodies (Sigma-Aldrich Co. ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... The membranes were then washed 3 × 5 min each with TBST and incubated with anti-rabbit IgG – HRP secondary antibody (abcam 6721) at 1:3000 in 5% BSA – TBST for 2 h at room temp ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Tendon stromal cells isolated from healthy volunteers and patients with tendon tears (n=3 each) were incubated with the following primary antibodies: anti-ALX (Abcam, ab26316), anti-ALOX15 (Abcam ab119774) ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Cell Biology 2020Quote: ... the ovarian samples were incubated overnight at 4°C with primary antibodies against caspase-3 (1:1000 dilution, Abcam, Cambridge, MA) and NLRP3 (1:1000 dilution ...