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Citations for abcam :
2351 - 2400 of 10000+ citations for 7 Fluoro 6 amino 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... The membranes were then washed 3 × 5 min each with TBST and incubated with anti-rabbit IgG – HRP secondary antibody (abcam 6721) at 1:3000 in 5% BSA – TBST for 2 h at room temp ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Tendon stromal cells isolated from healthy volunteers and patients with tendon tears (n=3 each) were incubated with the following primary antibodies: anti-ALX (Abcam, ab26316), anti-ALOX15 (Abcam ab119774) ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242; P2X7R, 1:50, Abcam/ab109054; S100β, 1:200, Abcam/ab52642; NeuN, 1:200, Millipore/MAB377) overnight at +4°C ...
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bioRxiv - Genomics 2023Quote: ... primary antibodies (CTCF: D31H2 CST 1:600, MRE11: ab214 Abcam 1:600, PCNA: ab29 Abcam 1:500, RAD21: ab992 Abcam 1:500, STN1: sc-376450 1:200) were added in a 1% BSA in PBS solution overnight at 4°C or RT for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... ALP (1:1000, A5111), SHH (1:1000, A5115) (Selleckchem), collagen □ (1:1000, ab255809), OCN (1:1000, ab133612), Runx2 (1:1000, ab54868) (abcam, UK), Ptch1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... EEA1 (1:200, CST 3288T), Lamin A/C (1:200, CST 4777T), Clathrin (1:200, CST, Synaptophysin (1:200, Abcam ab32127), Synaptotagmin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were procured from the following sources: β-catenin (1:1000, ab16051), CD31 (1:1000, ab222783), podoplanin (1:500, ab10288) and Beclin1 (1:2000, ab62557) from Abcam. Has2 (sc-514737 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000; EpiGentek A4032) (LAMP1 1:1000; Abcam ab32356) diluted in 5% BSA 0.5% Tween20 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Claudin-1 (Cldn-1) (1:200 prepared in 2.5% goat serum, Cat. No. ab15098; Abcam) for overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... samples were stained using primary antibodies: anti-Nkx2-1 (TTF-1) (1:500, ab76013, Abcam) and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:50; Abcam, Cat #: ab5076), mouse anti-α-syn phosphorylation at serine position 129 (p129 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-insulin (ab7842, 1:400), anti-glucagon (ab10988, 1:400), anti-somatostatin (ab30788, 1:100), and anti-Ki67 (ab15580, 1:1000) (Abcam, Cambridge, UK). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS, 1:50, Abcam; goat anti-DCX ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled for amyloid precursor protein (APP) (Chemicon, 1:1000), MAC3 (Pharmingen, 1:400), glial fibrillary acidic protein (GFAP) (Novo Castra, 1:200) or IBA1 (abcam, 1:1000). Images were acquired at 40x magnification using a bright-field light microscope (Zeiss AxioImager Z1 ...
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bioRxiv - Immunology 2022Quote: ... the sections were incubated overnight at 4°C with polyclonal primary antibody for ionized calcium-binding adapter molecule 1 (IBA-1; 1:400; Wako Chemicals, Richmond, VA, USA) or antibody for proliferation marker (Ki-67; 1:400; Abcam, Cambridge, UK). The sections were washing with PBS and incubated with the appropriate secondary antibody solution for 2h at room temperature (IgG conjugated Alexa Fluor 594 and/or 647 ...
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bioRxiv - Physiology 2022Quote: ... Primary antibodies (Slc2A1: Abcam ab15309 1:500, Slc2A3: Abcam ab15311 1:200, Slc38A1: Abcam ab104684 1:200, Slc38a2: Abcam ab90677 1:200) were then applied for 1-2 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... which were then incubated with the primary antibodies ERK5(#3372,CST,1:1000),Phospho-ERK5(Thr218/Tyr220)(#3371,CST,1:1000),c-Fo s(#4384,CST,1:1000),MEK5(ab45146,Abcam,1:5000),phospho-MEK5(S311/T315)(# PA5-37701,Thermo Fisher,1:1000) ...
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bioRxiv - Pathology 2021Quote: ... Membranes were stained for aSyn (pS129-aSyn, EP1536Y, 1:1000, Syn-1, 1:5000 and C-terminal directed MJRF1 [aa119-123, Cat# ab138501, Abcam], 1:5000) in Tris buffered saline with Tween-20 supplemented with 0.05% Sodium Azide overnight at 4°C and labelled with secondary antibodies (goat anti-mouse HRP or goat anti-rabbit-HRP ...
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bioRxiv - Cell Biology 2021Quote: ... 1:800), anti-OCN (ab93876, 1:500), anti-ALP (ab229126, 1:1000), and anti-RANKL (ab45039, 1:300) antibodies (Abcam, Cambridge, Britain), as described previously.(Luo et al ...
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bioRxiv - Genetics 2022Quote: ... anti-MKI67 (1:100, ab92742), anti-calponin (1:100, ab197639), anti-TOMM20 (1:200, ab56783) and anti-ACTA2 (1:500, ab781) (Abcam, Boston, MA), anti-COL4A5 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Standard immunocytochemistry techniques were used to stain cells with the following antibodies (anti-FLAG M2 clone 1:200; anti-TOMM40 1:200 Proteintech cat# 18409-1-AP; anti-LRPPRC 1:200 Abcam cat# Ab97505). Secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were permeabilized and blocked (same staining kit) prior to incubation with anti GFP antibody (abcam, ab13970, 4 °C).
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898 ...
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bioRxiv - Molecular Biology 2019Quote: ... BRCA2 depletion was checked on 4-12% Tris acetate gels according to the manufacturer’s instructions and membranes were probed with anti-BRCA2 (#ab9143, Abcam). Immunoreactivity was visualized using an enhanced chemiluminescence detection kit (ECL ...
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bioRxiv - Microbiology 2019Quote: ... Samples were drained and incubated for 16–18 h at 4°C with a primary antibody (Abcam Cat# ab1791, RRID:AB_302613, Abcam Cat# ab5103, RRID:AB_304752, or Abcam Cat# ab21595, RRID:AB_446409), diluted 1:200 in 2% goat serum/1% BSA in PBS) ...
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bioRxiv - Biochemistry 2019Quote: ... The remainder of the samples were incubated overnight at 4°C with anti-CENP-A antibody (Abcam cat #ab13939) in an end-over-end rotator before being imaged by AFM ...
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bioRxiv - Immunology 2019Quote: ... then incubated in secondary antibodies in 0.1% Triton X-100/PBS for 2 hr at 4°C (goat anti-rat Alexa 488, Abcam, ab150157 at 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with non-fat dry milk and incubated overnight with primary antibodies at 4°C (Abcam polyclonal rabbit IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194 ...