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Citations for abcam :
2301 - 2350 of 2400 citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed at least three times with PBST and incubated with native Streptavidin protein DyLight 488 (1:750 dilution; Abcam, ab134349) in 0.5% fish skin gelatin with PBST for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the included Pierce™ Protein A/G Plus agarose beads were incubated with rabbit anti-myc polyclonal antibody (ab9106, Abcam, USA) and cross-linked using 2.5 mM DSS crosslinker ...
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bioRxiv - Biochemistry 2023Quote: ... A small aliquot of the collected fractions was loaded on to an SDS–PAGE gel and stained with InstantBlue Coomassie protein stain (abcam #ab119211) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a fresh Eppendorf tube and the protein concentration was assessed using Bradford reagent (Abcam, cat#ab119216) and stored at -80°C when not used immediately ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... were coupled with 10μg of anti-protein of interest (POI; anti-Foxg1 ChIP-grade, rabbit polyclonal, Abcam #ab18259; anti-GFP, rabbit polyclonal, Abcam #ab290), or 10μg of rabbit IgG (Millipore #12370 ...
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Genomics 2023Quote: ... The heterozygous expression of the Dam-Lamin B1 fusion protein was further confirmed by Western blot using an anti-HA antibody at 1 in 5000 dilution (Abcam, ab9110) and an anti-LaminB1 antibody at 1 in 5000 dilution (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Microbiology 2023Quote: ... Cells were blocked with Dako protein block then stained for cell surface Env with 2G12 anti-gp120 directly conjugated to APC (Abcam, ab201807) and diluted 1:100 in Dako antibody diluent (10ug/mL final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Pathology 2023Quote: ... CEBP/α (diluted 1:400, Huabio, EM1710-20, CA) and fatty acid-binding protein (FABP4, diluted 1:200, abcam, ab92501, UK) overnight at 4□ ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragmented chromatin fragments were pre-cleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti-H3K36me2(Abcam, ab9049) or anti-p65 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... previously blotted membranes from Figure S2C,E were re-probed with 1:5000 mouse monoclonal anti-TATA binding protein antibody (Abcam #ab51841). The membrane in Figure S2H was probed for 1 h with 1:1000 rabbit anti-LARP7 antibody (Fortis/Bethyl #A303-723A ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled for amyloid precursor protein (APP) (Chemicon, 1:1000), MAC3 (Pharmingen, 1:400), glial fibrillary acidic protein (GFAP) (Novo Castra, 1:200) or IBA1 (abcam, 1:1000). Images were acquired at 40x magnification using a bright-field light microscope (Zeiss AxioImager Z1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Molecular Biology 2021Quote: ... autoradiography recorded overnight using a phospho-imaging system and protein bands detected by Western blot using anti-TRIM25 (ab167154, Abcam, 1:2000).
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Pathology 2019Quote: ... Soluble proteins (20 μg) were used for Western blotting with primary antibodies against AKT1/2/3 (rabbit monoclonal antibody; EPR16798 Abcam; 1:10,000), mTOR (rabbit monoclonal antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Neuroscience 2020Quote: ... cell lysates were collected in parallel with the supernatants from the same wells and then tested for levels of a housekeeping protein beta-actin (Ab8227, Abcam, RRID: AB_2305186) by western blot ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407 ...
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bioRxiv - Cell Biology 2022Quote: ... The relative amount of each of these muscle proteins in each lane was normalized to the amount of Histone H3 detected using anti-Histone H3 (abcam, cat. ab1791) at 1:40,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... or GT1-7MC4R cells (50 μg of protein) were subjected to immunoprecipitation overnight with 1 μg of a specific α2 AMPK antibody (ab3760, Abcam, Cambridge, MA) coupled with protein G-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...