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Citations for abcam :
2301 - 2350 of 2834 citations for 8 4 Chlorophenoxy 2 methylideneoctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked in 10% milk in 1xTBSTw overnight at 4°C and incubated with rabbit anti-GAPDH primary antibody (1:1000, abcam, catalog ab9495) and processed as described above.
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bioRxiv - Cell Biology 2019Quote: ... membranes were incubated over night at 4°C with mouse anti-phosho-PP1 antibody (Wu et al., 2009) (pospho-T320 Abcam, 1:1000) After washing ...
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bioRxiv - Neuroscience 2019Quote: ... and 2% goat serum and free-floating slices were then incubated with the following primary antibodies at 4°C overnight: rabbit anti-DCX (Abcam, ab 18723), rabbit anti-Ki67 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... then were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature and stained using the Senescence-Associated β-Galactosidase Staining Kit (Abcam ab65351), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Genetics 2021Quote: ... The blocked membranes were further washed three times with TBST buffer and incubated at 4°C overnight with primary antibodies CDKN2A/p16INK4a (ab108349, Abcam, 1:1000), β-actin (Santa Cruz β-actin (C4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4 °C with primary antibodies prepared in blocking buffer against phospho-MLKL (Ser345) (Abcam, Cat. No. ab196436), MLKL (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized and then incubated with blocking solution (5% normal goat serum in 0.1% Triton X-100 in PBS) for 1 hr followed by primary antibody incubation overnight at 4°C using goat Anti-nNOS (ab1376, Abcam, 1:300) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then incubated overnight in humidified chambers at 4°C in the following primary antibodies diluted in blocking buffer: anti-MAP2 (1:700; Ab5392, Abcam, Cambridge, UK), SMI312 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and then incubated with the following primary antibodies (diluted in 5% non-fat milk in 0.1% PBS-Tween 20) for 18 hrs at 4°C: Mouse monoclonal Total OXPHOS rodent WB cocktail (Abcam ab110413, 1:1,000); mouse monoclonal Total OXPHOS blue-native WB cocktail (Abcam ab110412 ...
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bioRxiv - Physiology 2020Quote: ... Cells were washed 3 × in TBS and incubated overnight (4°C) in 300 μl of anti-desmin (Abcam Cat# ab15200, RRID: AB_301744) primary antibody made up in TBS ...
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bioRxiv - Systems Biology 2021Quote: ... prior to overnight treatment at 4 °C with rabbit anti-murine primary antibodies raised against GFAP (1:1000, ab7260, Abcam Ltd, UK) or Iba1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were then washed with PBS and incubated for 24 h at 4 °C with anti-BDNF (Abcam, USA, 1:400), anti-doublecortin (DCX ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in 2.5% BSA/PBS and cells were incubated overnight at 4°C in a humidified chamber with primary antibodies anti-GFP (Abcam, 1:5000), anti-GluA1 (homemade ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Neuroscience 2020Quote: ... Then the tissue sections were incubated overnight at 4 degrees Celsius with the primary goat polyclonal to cfos (1:500, 24 h, abcam; RRID: SCR_012931) primary antibody ...
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bioRxiv - Microbiology 2019Quote: ... The cells were incubated overnight with primary antibodies at 4 °C (Anti-hCG beta/β-CG/hCGB3, Abcam #ab53087 and Anti-Cytokeratin 7/CK7/KRT7, Abcam #ab9021), washed with PBS and subsequently incubated with secondary fluorescent antibodies for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... slides were blocked with PBS + 1% BSA + 2% goat serum + 0.2% Triton X-100 for 1h at RT and incubated overnight at 4◦C with anti-DCLK1 antibody (Abcam #ab31704; 1:250) in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated at 4°C in c-Fos primary antibody (1:4000 Anti-c-Fos rabbit pAb; 190289; Abcam, Toronto, ON) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sections were incubated for 72 h at 4 °C with gentle agitation with rabbit polyclonal anti GLS2 (ab113509; 1:1000, Abcam, Cambridge, UK) and sheep polyclonal anti pS6 (ab65748 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked for 30 min with 3% BSA dissolved in Tris-buffered saline with 0.1% Tween (BSA-TBST) and incubated overnight at 4 °C with primary antibody (ab43177 chicken-developed anti-ATP synthase β, Abcam plc) diluted 1:5000 in BSA-TBST ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
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bioRxiv - Immunology 2020Quote: ... The sonicated nuclear fractions were divided for input control and for overnight incubation at 4℃ with 10 μg of either anti-C/EBPβ (Abcam cat. 32358) or the negative control IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500, Covance; TH, 1:1000, Chemicon; Serotonin, 1:500, Abcam). Negative primary controls consisted of cells treated with blocking buffer without the addition of primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... The retinas were incubated in PBS with 1% Triton X-100 and 0.5% Tween 20 for 1 h at room temperature and in 4% BSA for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies: RBPMS (1:500, Abcam, Cambridge, UK), ChAT (1:100 ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Genetics 2022Quote: ... After 6 h the cells were washed in 1XPBS and fixed in 4% PFA followed by staining with rabbit polyclonal beta COP antibody (ab2899, Abcam, Cambridge, UK) after permeabilising with 0.5% Triton-X 100 ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Immunology 2022Quote: ... for 30 minutes at 4°C and then incubated with the titrated primary anti-MPO-PE and primary citrullinated histone H3 (citH3) antibody (Abcam, Waltham, MA) for 30 minutes at 4° C ...
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bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were incubated overnight at 4°C in 1:100 dilution of rabbit polyclonal anti-nitro tyrosine antibody (ab42789; Abcam, Cambridge, MA). Slides were rinsed in PBS and developed using polyclonal goat anti-rabbit Immunoglobulins conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed 48 h post transfection with 4% paraformaldehyde (PFA) and co-stained with rabbit anti-HA (1:500) and Mouse anti-CD63 (1:200; Abcam, Cambridge, MA) primary antibodies and with Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
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bioRxiv - Cell Biology 2019Quote: ... the tissue was permeabilized with 2 washes of PBS0.5T (PBS + 0,5% v/v Triton X-100) for 1h and the samples were incubated overnight at 4°C with primary antibody against Keratin 14 (Abcam, 1:500) diluted in PBD0.2T (PBS + 1%BSA + 1% v/v DMSO + 0.2% v/v Triton X-100) ...
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated on an orbital shaker for 24h at room temperature + 24h at 4 °C in polyclonal rabbit anti-mCherry (1:30 000, Abcam, cat. ab67453) or polyclonal chicken anti-EYFP (1:200 000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...