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Citations for abcam :
2301 - 2350 of 2562 citations for 4 2 Trimethylsilyl Ethyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2020Quote: ... anti-LAMA 1&2 (ab7463, 1:1000), anti-CD59 (ab133707, 1:1000), and anti-GAPDH (ab9485, 1:2500) were purchased from Abcam.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Cell Biology 2022Quote: ... The pre-cleared supernatants were divided into two parts and incubated with 40 μL Dynabeads pre-bound with antibodies for 3.5 μg NONO or RNA polymerase II phosphorated at Serine 2 (Abcam, ab5095) and normal mouse IgG overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Developmental Biology 2022Quote: ... Blastocysts were then washed three times in PBS with 2% BSA and then incubated with a secondary anti‐rabbit antibody (1:200, ab150077, Abcam; 1:200, ab150075, Abcam) or anti-mouse antibody (1:200 ...
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bioRxiv - Neuroscience 2022Quote: Slides were similarly processed for BDNF with the exception that slides were blocked with 10% sheep serum in 5% Triton-X PBS under coverwells in a humid chamber for 2 hours and slides were incubated with primary antibodies for BDNF (1:500; ab226843, Abcam) and NeuN (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed three times with PBS and antibody binding was probed using secondary-conjugated Goat antibodies targeting the species of ALG-2 and ALIX antibodies (no. ab175652, ab150113, abcam). Secondary antibodies were diluted 1:500 with 2% BSA in PBS and incubated with cells in the dark for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody incubation was performed for 2 hours at room temperature with slow rocking using Goat Anti-Mouse IgG H&L (ab6789, Abcam). The blots were developed using Clarity Western ECL Substrate (1705061 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... the following antibodies and amounts of antibody were used for the 50,000-cell ChIP: 2 µg of FOXA1 (Abcam, ab170933), 2 µg of H3K4me1 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used in this study: anti-SARS-CoV-2 spike glycoprotein rabbit polyclonal antibody (dilution 1:1000; #ab272420, Abcam); anti-SARS-CoV-2 nucleocapsid protein rabbit polyclonal antibody (dilution 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated for 2 hrs with a rabbit polyclonal pan-synuclein antibody (1:1000; ab53726; Abcam, Cambridge, MA). After washing in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS was diluted in the supernatant to 0.1% with FA-lysis buffer and incubated with 2 μg anti-Rpb3 antibody (Abcam 1Y26) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 2% BSA in PBS for 1 hr and subsequently incubated with anti-androgen receptor antibody (1:400, Abcam) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-SQSTM1/p62 (IP 1-2 μg/mg of lysate; ab101266) and rabbit anti-GFP (WB 1:2000, ab290) were from Abcam; mouse anti-SQSTM1/p62 (WB 1:1000 IF 1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were washed 3 times with PB and incubated for 2 hours at room temperature with secondary antibodies (goat anti chicken Alexa Flour 594, abcam, ab150172 ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
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bioRxiv - Pathology 2021Quote: ... for 20 min before being incubated overnight with the primary anti-ACE-2 antibody (a polyclonal antibody raised in rabbits and diluted 1:2000, ab15348 Abcam) at 4°C in Tris-Buffered Saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 1x TBS and incubated for 2 hours at room temperature with appropriate secondary antibodies (goat anti-chicken FITC, 1:200, Abcam or goat anti-rat AF488 ...
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bioRxiv - Physiology 2020Quote: ... in 300 μl of TBS (with 2% goat serum and 0.2% Triton X-100) containing primary anti-desmin antibody (1:50; ab15200, Abcam, UK). After overnight incubation ...
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bioRxiv - Neuroscience 2020Quote: ... the conditioned media was given a short spin at 1000 rcf for 2 minutes to remove the cell debris and subjected to ELISA (ab108813, Abcam) to estimate the amount of APOE secreted by iPSCs.
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), CENP-E (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784, 1:500; PDHAl [9H9AF5], Abcam #ab110330 ...
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bioRxiv - Developmental Biology 2021Quote: ... mice were bred to Rosa26-mTmG (91) and analyzed at 2 months by immunostaining with chicken anti-GFP antibodies (Abcam) and Alexa Fluor 647 hydrazide ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in 2% BSA in PBS to block against non-specific binding and incubated in 2% BSA containing rabbit anti-LC3B primary antibody (Abcam). Coverslips were then washed 3 × 5 minutes in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with 200 μL DMEM and then incubated with a mixture of 2 μL Apopxin Green (Abcam) and 0.4 μL Propidium Iodide (15 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were subsequently subjected to 2 h of incubation with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody (Abcam). Immunoblots were visualized by enhanced chemiluminescence (ECL kit ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 2% BSA in PBS and then incubated with anti- H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 2% BSA in PBS for 30 min and cells were incubated with phalloidin-iFluor 594 reagent (1:1000; ab176757; Abcam) for 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were blocked with goat serum for 2 hours at room temperature and incubated with RAD51(1:500 no. ab133534 Abcam) or yH2AX (1:200 no ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Neuroscience 2023Quote: ... were washed with PBS + 0.1% Tween-20 (PBS-T) then incubated with 2 µL of PDI antibody (Abcam, Catalog #ab2792) in 200 µL PBS-T for 10 min (end-over-end rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107 ...
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bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in 1xPBS with 0.05% Triton and incubated for 2 hours at room temperature with a secondary antibody (Abcam, ab150077). Brain slices were washed and mounted in Fluoromount (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 2 hours incubation with respective Horseradish peroxidase-coupled secondary antibodies (anti-mouse IgG ab97023 and anti-rabbit IgG ab97051, Abcam). Luminescence was then detected using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...