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Citations for abcam :
2151 - 2200 of 3387 citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... all samples were analyzed with a sensitive ELISA-kit (#ab157715, Abcam) according to the manufacturer’s instructions and as previously described72 ...
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bioRxiv - Microbiology 2024Quote: Total ammonium was quantified colorimetrically using Ammonium Assay Kit (Abcam, 83360) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Aconitase activity was measured using the Aconitase assay kit (ab83459, Abcam). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Anti-Respiratory Syncytial Virus (RSV) IgG Human ELISA Kit (Abcam, ab108765), SARS-CoV-2 Spike Protein Serological IgG ELISA Kit (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: Intracellular citrate concentration was measured using the Citrate Assay Kit (Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were again washed thrice in 0.1 M PB and then incubated with the secondary antibody for 2 h (goat anti-chicken-IgY conjugated to Alexa Fluor 594, Abcam, ab150172). Following the incubation with the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature (Chicken anti-GFP, Aves cat#GFP-1020, RRID:AB_10000240; Rabbit anti-NUP153, Abcam cat#ab84872, RRID:AB_1859766), followed by washing 3x 5 min in 1x PBS ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Cell Biology 2021Quote: Total intracellular ROS contents of cultured HCs were measured using the fluorescent probe 2’,7’-dichlorofluorescin diacetate (DCFDA) (ab113851, Abcam, UK) (Lv et al. ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
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bioRxiv - Cell Biology 2022Quote: ... Epitope detection was conducted with a primary monoclonal mouse antibody against NONO at 1:500 dilution in PBST and a polyclonal rabbit antibody against RNA Pol II phosphorated at Serine 2 (Abcam, ab5095) at 1:500 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed in distilled water and stained for 15 min in 2% w/v Alizarin Red (ab146374, Abcam, Cambridge, UK) in distilled water at a pH of ~4.2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.7% Triton X-100 in 1X PBS) for 2 hours at room temperature and incubated in blocking solution with anti-GFP antibody (1:500, Abcam, ab6658) overnight at 4 ºC ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 1 h with primary antibodies (dilution in 10% goat serum in PBS) to p-Pol 2(S5) (1:1000 dilution; Abcam, #ab5408) and H3K27ac (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Cell Biology 2021Quote: ... and reacted with an antibody to synaptic vesicle 2 (SV2 1:50; Developmental Studies Hybridoma Bank Ab2315387) and neurofilament 200 (NF200 1:500, Abcam Ab72996) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS; Abcam, #ab7475) prepared in 1% BSA in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with blocking solution (TBS containing 5% normal goat serum and 0.2% Triton X-100) for 2 hr and incubated overnight at 4°C with chicken anti-GFP monoclonal antibody (1:500; abcam; ab13970). After washing three times with TBST (TBS containing 0.2% Triton X-100) ...
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were blocked with 5% nonfat milk in TBST for 2 hours at room temperature and incubated with primary antibodies specific for Osx (1:1000, Abcam, UK), Runx2 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Laminin (1:500, DSHB 3H11), mouse anti-Msx1/2 (1:100, DSHB 4G1) and rabbit anti-RFP (1:500, Abcam ab62341).
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bioRxiv - Bioengineering 2021Quote: ... diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Standard Western Blot analysis using α-B3GLCT (IHC-plus antihuman B3GALTL antibody, 1:500) and α-rabbit-HRP (2°, 1:5,000, Abcam)] was performed 9 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Genetics 2021Quote: ... Cells were incubated with primary antibody (1:400 RNAPII [pSer2] Novus Biologicals NB100-1805, 1:400 PCNA [5E6/2] abcam ab70472) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Baltimore PKD Center), anti-PC-2 monoclonal antibody (3374 CT-1 414, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein tech 50430-2-AP), anti-RFP (rabbit, 1:3 000, St. Johns Laboratory STJ97083) and anti-H2B (mouse, 1:3 000, Abcam, ab52484). Membranes were washed 3 times for 10 min each in PBST ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...