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Citations for abcam :
2151 - 2200 of 2792 citations for 6 Fluoro 2 methylamino 4 1H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Incubation with primary antibodies diluted in PBS with 5 % NGS and 0.3 % Triton X-100 was carried out at 4°C overnight using rabbit anti-DCX (ab18723, Abcam, 1:1000), mouse anti-parvalbumin (p3088 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated overnight with primary antibodies diluted in 1% BSA-TBST at 4°C using the following concentrations: anti-PAD4 (1:1000; Abcam; ab214810), anti-PAD2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then processed for immunostaining by overnight incubation at 4°C in the primary antibodies of anti-Human phospho alpha-synuclein (Ser129) (1:2000, Cat# ab51253, Abcam, RRID:AB_869973), anti-Iba-1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoblotting was done overnight at 4°C with the following antibodies at the appropriate dilutions: TRPC6 antibody (Abcam ab228771, 1:1000). The blots were washed the next day and incubated with Goat anti-Rabbit-HRP secondary antibody (Cat # 31460 ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C with rabbit anti-aromatase (1:2000, Abcam cat # ab18995), chicken anti-TH (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed twice with PBS before incubation overnight at 4°C with chicken anti-TH (1:2000, Abcam cat# ab76442) in 1% NGS + 0.05% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was blocked for nonspecific binding with 5% milk in 1x TBST (Tris-buffered saline, 0.1% Tween 20) and incubated with rabbit polyclonal anti-4-HNE (ab46545, Abcam, 1:1500) and HIF-1α (141795 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then incubated at 4°C overnight in blocking buffer with the following antibodies and concentrations: chicken anti-GFP (1:1000; Abcam ab13970), rabbit anti-per (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies (anti-GFAP 1:1000, Invitrogen #PA1-10004; anti-ALDH1L1 1:1000, Abcam ab87117) diluted in PBS with 1 % bovine serum albumin (Winset Inc ...
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bioRxiv - Cell Biology 2024Quote: ... some of the following primary antibodies were incubated 24 hours at 4 °C: rabbit anti-SOX2 (1:100 dilution, Abcam, ab137285), rabbit anti-doublecortin (DCX ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 24 hours at 4 ° C with the following primary antibodies: rabbit anti-syntenin 1 (1:2000 dilution, Abcam, ab133267) and mouse anti-tubulin α (1:2000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated overnight at 4°C with primary antibodies diluted in 5% BSA: rabbit α-SYCP1 (Abcam, ab15090; 1:200) and mouse α- γH2AX (Millipore Sigma ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of total fragmented chromatin was saved as an input control and the remaining chromatin was split into two tubes (representing 5×107 cells each) for antibody pulldown and incubated overnight at 4°C with 15 ug of NRF1 (Abcam #ab55744), or MYC (Abcam #ab56 ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% lowfat milk-PBS-tween solution and then incubated overnight at 4 °C with primary antibodies: anti-PURA 1:1500 (rabbit—ab125200 abcam®), anti-GLUT1 1:2000 (mouse— [GLUT1/2476] (A249979 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Biochemistry 2023Quote: Rat sera were incubated at 4°C for 16 h in the presence of anti-adiponectin antibodies from Abcam (ab 56416) and Cell Signaling (2789s) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in PBS with 3% donkey serum and the following primary antibodies for 72 h at 4°C: goat anti-Iba1 (1:500; Abcam, ab5076), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C in a blocking-permeabilizing solution containing rabbit anti-SOX10 (1:100, Abcam, ab227680). The next day ...
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bioRxiv - Genomics 2023Quote: ... in PBS-T for 1 hour and incubated overnight in 5% goat serum in PBS-T with primary antibody at 4°C (SON (1:300, abcam ab121759) or SRRM2 (1:300 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated overnight at 4 °C with rabbit anti-Bru1 (1:500) (38) or rabbit anti-GFP (1:1000, Abcam ab290). All antibodies are listed in the Resources Table S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were removed and nuclear extracts were incubated overnight at 4°C with either anti-lamin A/C antibody (Abcam, #ab108595) or non-immune IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST, D10A8) or SOX2 (Abcam, ab97959), or isotype control antibodies (rabbit IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections then were incubated at 4°C overnight with the following primary antibodies at these concentrations: aSyn (Abcam ab209420, 1:1000), phosphorylated aSyn (Wako 015-25191 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and resuspended in PBS and subsequently incubated with 4 μg/ml of PE-conjugated GABARAP/GABARAPL1/GABARAPL2 antibody (Abcam, ab223948) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Triton X-100) and incubated overnight at 4°C with primary antibody: anti-αSMA (1:100, Abcam cat. no. ab7817), anti-Periostin (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were added at 1:200 dilution in PFS and incubated overnight at 4°C with gentle shaking: Collagen IV (Abcam ab19808), pAKT pS473 (CST ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): anti-CD146 (Abcam #ab75769); anti-#-SMA (Abcam #ab5694 ...
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bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with primary antibodies phospho-STAT3-Tyr705 (Cell Signalling, 9145S), STAT3 (Cell Signalling, 4904S) and Twist1 (Abcam, ab50887) raised in rabbit ...
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bioRxiv - Pathology 2023Quote: ... The rat monoclonal anti-mouse dipeptidyl peptidase-4 (Dpp4 also known as CD26) and the rat monoclonal anti-mouse F4/80 antibodies were purchased from Abcam (ab42899) and AbD serotec (CL:A3-1) ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were stained overnight at 4°C with the following primary antibodies: anti-ORC1 (1:1000 TBST/5% milk; Abcam, ab85830). Immunoblots were then incubated in secondary goat anti-rabbit (1:1000 TBST/5% milk ...
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bioRxiv - Microbiology 2024Quote: ... which was then washed in PBS with 0.1% Tween-20 (PBS-T) and incubated overnight at 4°C shaking in PBS-T with rabbit anti-GAC antibodies (Abcam ab21034) at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Neuroscience 2023Quote: ... and membranes were blocked with 5% skimmed milk in TBST buffer for 1 h and incubated overnight at 4 °C in TBST with the following primary antibodies: Anti-rabbit TOMM20 (Abcam #ab186735) 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of GBO medium containing 1 mM ACh with or without a combination of the M3 muscarinic receptor antagonist 4-DAMP (100 μM) and pan-nicotinic receptor antagonist mecamylamine (100 μM, Abcam, ab120459) was added to each GBO at the ALI surface 30 minutes prior to imaging with the same conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C in PBS with primary antibodies including: chicken-anti-GFP (1:10000, Abcam, ab 13970) or rabbit-anti-dsRed (1:1000 ...